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The map label for this gene is recA

Identifier: 59711142

GI number: 59711142

Start: 577375

End: 578421

Strand: Direct

Name: recA

Synonym: VF_0535

Alternate gene names: 59711142

Gene position: 577375-578421 (Clockwise)

Preceding gene: 172087669

Following gene: 59711143

Centisome position: 19.93

GC content: 40.69

Gene sequence:

>1047_bases
ATGGACGATAACAAGAAAAAAGCACTGGCCGCCGCATTAGGTCAAATTGAAAAACAATTTGGTAAAGGTTCAATCATGAA
GTTGGGCGATAACCGCACAATGGATGTTGAAACTGTGTCTACTGGTTCTTTATCTCTTGATATTGCTCTAGGTGCTGGTG
GTTTACCAATGGGTCGTATCGTTGAGATTTTTGGCCCTGAGAGTTCAGGTAAAACAACACTGACTCTAGAATTAATTGCA
GCTGCGCAACGTGAAGGTAAAACATGTGCCTTTATTGATGCTGAACATGCTCTAGATCCTGTATACGCTAAAAAACTTGG
CGTTAATATTGATGAATTATTAGTTTCTCAGCCAGATACGGGTGAGCAAGCTTTAGAAATCTGTGATGCGTTAGCACGTT
CTGGTGCTGTTGATGTAATGGTAATTGACTCCGTTGCAGCATTAACACCAAAAGCTGAAATTGAAGGTGAAATGGGCGAT
AGCCACATGGGTCTTCAAGCTCGTATGCTTTCTCAAGCAATGCGTAAATTAACGGGTAACCTAAAGCAGTCAAACTGTAT
GGCTATCTTCATTAACCAAATTCGTATGAAGATTGGTGTAATGTTTGGTAACCCTGAAACAACAACTGGTGGTAATGCAC
TTAAGTTCTATGCATCTGTTCGTCTTGACATTCGTCGTACTGGCGCAGTAAAAGATGGCGATGAAGTTGTTGGTAACGAA
ACTCGTATCAAAGTCGTTAAGAATAAAATTGCAGCACCATTTAAACAAGCTGAAACTCAAATCATGTACGGCCAAGGTTT
TAACCGTGAAGGTGAGTTAATTGACTTAGGTGTTAAACATAAGTTGGTTGATAAAGCGGGCGCATGGTACAGCTACAATG
GTGATAAGATTGGTCAAGGTAAAGCGAACGCATCGAAATTCATGCGTGAAAATACTGAAGTAGCAGCAGAACTAGATAAG
AAGCTACGTGAGATGCTATTGACTCCAGCAGAAGAAAAGCCAGAGACTGATGCTGCTCCTGAAATTGAAGAAAACGAAGA
GTTTTAA

Upstream 100 bases:

>100_bases
AAAATTAGAAAAAAAATAGCAAAGCTATAGACACTGTACAAATAGACAGTATACTACTGGTCATTAACTGATTTAGTGAT
CTAGAAATCTGGAGAATCAA

Downstream 100 bases:

>100_bases
GCTTTAGTTTCTCTATAATATTTTTTAAAAGCCAAACCTTAGTGTTTGGCTTTTTTATATCTAGCTATTTGTATTCTTAA
GACGTTTCTTCAAATCCCTC

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDDNKKKALAAALGQIEKQFGKGSIMKLGDNRTMDVETVSTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVMVIDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMAIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAVKDGDEVVGNE
TRIKVVKNKIAAPFKQAETQIMYGQGFNREGELIDLGVKHKLVDKAGAWYSYNGDKIGQGKANASKFMRENTEVAAELDK
KLREMLLTPAEEKPETDAAPEIEENEEF

Sequences:

>Translated_348_residues
MDDNKKKALAAALGQIEKQFGKGSIMKLGDNRTMDVETVSTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVMVIDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMAIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAVKDGDEVVGNE
TRIKVVKNKIAAPFKQAETQIMYGQGFNREGELIDLGVKHKLVDKAGAWYSYNGDKIGQGKANASKFMRENTEVAAELDK
KLREMLLTPAEEKPETDAAPEIEENEEF
>Mature_348_residues
MDDNKKKALAAALGQIEKQFGKGSIMKLGDNRTMDVETVSTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVMVIDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMAIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAVKDGDEVVGNE
TRIKVVKNKIAAPFKQAETQIMYGQGFNREGELIDLGVKHKLVDKAGAWYSYNGDKIGQGKANASKFMRENTEVAAELDK
KLREMLLTPAEEKPETDAAPEIEENEEF

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=351, Percent_Identity=78.9173789173789, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_VIBF1 (Q5E7G6)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203918.1
- ProteinModelPortal:   Q5E7G6
- SMR:   Q5E7G6
- STRING:   Q5E7G6
- GeneID:   3277128
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0535
- NMPDR:   fig|312309.3.peg.535
- eggNOG:   COG0468
- HOGENOM:   HBG339889
- OMA:   CEYLREN
- PhylomeDB:   Q5E7G6
- ProtClustDB:   PRK09354
- BioCyc:   VFIS312309:VF0535-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 37588; Mature: 37588

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDNKKKALAAALGQIEKQFGKGSIMKLGDNRTMDVETVSTGSLSLDIALGAGGLPMGRI
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCEEEEEEECCCCCCHHHE
VEIFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDT
EEEECCCCCCCEEEEEEEEHHHHCCCCEEEEEEHHHHCCHHHHHHHCCCHHHHHCCCCCC
GEQALEICDALARSGAVDVMVIDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGN
HHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
LKQSNCMAIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAVKDGDEVVGNE
CCCCCCHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHCCCC
TRIKVVKNKIAAPFKQAETQIMYGQGFNREGELIDLGVKHKLVDKAGAWYSYNGDKIGQG
CEEEEHHHHHCCCHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHCCCEEEECCCCCCCC
KANASKFMRENTEVAAELDKKLREMLLTPAEEKPETDAAPEIEENEEF
CHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDDNKKKALAAALGQIEKQFGKGSIMKLGDNRTMDVETVSTGSLSLDIALGAGGLPMGRI
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCEEEEEEECCCCCCHHHE
VEIFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDT
EEEECCCCCCCEEEEEEEEHHHHCCCCEEEEEEHHHHCCHHHHHHHCCCHHHHHCCCCCC
GEQALEICDALARSGAVDVMVIDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGN
HHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
LKQSNCMAIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAVKDGDEVVGNE
CCCCCCHHHHHHHHEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHCCCC
TRIKVVKNKIAAPFKQAETQIMYGQGFNREGELIDLGVKHKLVDKAGAWYSYNGDKIGQG
CEEEEHHHHHCCCHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHCCCEEEECCCCCCCC
KANASKFMRENTEVAAELDKKLREMLLTPAEEKPETDAAPEIEENEEF
CHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA