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The map label for this gene is radA [H]

Identifier: 59711135

GI number: 59711135

Start: 564644

End: 566026

Strand: Direct

Name: radA [H]

Synonym: VF_0528

Alternate gene names: 59711135

Gene position: 564644-566026 (Clockwise)

Preceding gene: 59711130

Following gene: 59711136

Centisome position: 19.49

GC content: 42.52

Gene sequence:

>1383_bases
ATGGCAAAAGCAAAACGCGCCTACGTTTGTAATGATTGTGGGGCAGACTTCCCTCGTTGGCAAGGACAGTGTAATGCTTG
TGGGGCATGGAACACGATTACAGAGGTTCGTTTGGCTGCTTCTCCACAAGTTGCTCGTAATGAACGTTTATCTGGTGGTT
ATGCGGGCTCCACTACAGATTCAGAAGTTCAAACTTTATCGAGTATTGATTTACAAGAAGTTCCTCGGTTCAGTAGTTCG
TTTAAAGAGTTTGACCGAGTTCTCGGTGGTGGCATTGTTCCTGGTGCAGCAATTTTGATTGGTGGTAATCCGGGTGCTGG
TAAATCTACTTTACTTTTGCAAACTATGTGTCGTTTGGCTGCAGAAATGCCAACATTGTATGTTACTGGTGAAGAATCGT
TACAACAGGTAGCAATGCGAGCTTCTCGTCTTGGGTTGCCAAAAGATAATCTGAAAATGTTATCTGAAACCAACATAGAT
AAGATTTGTCAGGTTGCTGAGAAAGAACAGCCAAAAATAATGGTTATTGACTCTATTCAAGTAATGCATGTTGCTGATGT
TCAATCATCTCCAGGCAGCGTCGCGCAGGTTCGTGAATCAGCAACTGCTTTAACTCGATATGCTAAGCAAAATAATGTGG
CTATTTTCTTAGTTGGTCATGTAACCAAAGATGGTACTTTAGCAGGCCCTAAAGTGTTGGAGCATATTATTGACTGTTCT
GTGTTACTTGACGGTAGTGCAGATAGTCGCTTTAGAACGCTTCGTAGTCATAAAAACCGTTTTGGAGCTGTTAATGAGCT
TGGGGTTTTTGCTATGACAGGCCAAGGCTTAAAAGAAGTAAATAACCCATCAGCAATTTTCTTATCTCGTGGAGAAGAGG
AAACATCTGGTAGTTCAGTGATGGTTGTGTGGGAAGGAACACGCCCGTTACTTGTAGAGATACAAGCCTTAGTGGATTAC
TCTCAGCTTTCAAATCCTCGTAGAGTCTCTGTTGGCTTAGATCAAAACCGTTTATCTATGCTACTCGCGGTTCTACATAA
GCATGGTGGTTTGCAAATGGCTGACCAAGATGTGTTTGTTAACGTGGTTGGCGGCGTTAAGGTAGCTGAAACCAGTGCGG
ATTTGGCACTTATTATGGCGTTACTATCTAGTTTTAAAGACCGTCCATTAGCTAAGGATGTAGTGGTCTTTGGTGAAGTT
GGTCTTGCTGGTGAGATTCGCCCAGTTCCAAGTGGGCAAGAGCGTTTAATGGAAGCATATAAACATGGCTTCAAAAAAGC
GATTGTACCTGCTGCAAACATGCCAAAAGGTGGCATTGAAGGAATGCAAATTCACGGCGTTAAGAAACTATCTGAAGCAA
TTGATGCTTTTGATGAACTTTAA

Upstream 100 bases:

>100_bases
AATCTAGTGGGTTTATCGGCAGAATTGCTCGTTTATTTAACAATGATACGTTAAATAGTTATAACCCATCACTTTTATTT
GATTTATATGTCGGTGATAT

Downstream 100 bases:

>100_bases
CTGAGGCTTTGAATTCGGATTTCTGCATTATTTTATTTGAAAATACAGAAATTCATCTATAATTTTCAGGGGCAGAGGCC
ACATTAGAACCATTTTGGTA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGGYAGSTTDSEVQTLSSIDLQEVPRFSSS
FKEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLAAEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETNID
KICQVAEKEQPKIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAIFLVGHVTKDGTLAGPKVLEHIIDCS
VLLDGSADSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDY
SQLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVNVVGGVKVAETSADLALIMALLSSFKDRPLAKDVVVFGEV
GLAGEIRPVPSGQERLMEAYKHGFKKAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL

Sequences:

>Translated_460_residues
MAKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGGYAGSTTDSEVQTLSSIDLQEVPRFSSS
FKEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLAAEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETNID
KICQVAEKEQPKIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAIFLVGHVTKDGTLAGPKVLEHIIDCS
VLLDGSADSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDY
SQLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVNVVGGVKVAETSADLALIMALLSSFKDRPLAKDVVVFGEV
GLAGEIRPVPSGQERLMEAYKHGFKKAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL
>Mature_459_residues
AKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGGYAGSTTDSEVQTLSSIDLQEVPRFSSSF
KEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLAAEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETNIDK
ICQVAEKEQPKIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAIFLVGHVTKDGTLAGPKVLEHIIDCSV
LLDGSADSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDYS
QLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVNVVGGVKVAETSADLALIMALLSSFKDRPLAKDVVVFGEVG
LAGEIRPVPSGQERLMEAYKHGFKKAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=461, Percent_Identity=80.6941431670282, Blast_Score=773, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49391; Mature: 49260

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGGYAGSTTD
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCHHHCCCCCCCCCCCCCH
SEVQTLSSIDLQEVPRFSSSFKEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLA
HHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETNIDKICQVAEKEQPKIMVIDSIQ
HHCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEE
VMHVADVQSSPGSVAQVRESATALTRYAKQNNVAIFLVGHVTKDGTLAGPKVLEHIIDCS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCCE
VLLDGSADSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSV
EEECCCCCHHHHHHHHHHHHCCCHHHCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEE
MVVWEGTRPLLVEIQALVDYSQLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFV
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCEEE
NVVGGVKVAETSADLALIMALLSSFKDRPLAKDVVVFGEVGLAGEIRPVPSGQERLMEAY
EEECCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKKAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL
HHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGGYAGSTTD
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCHHHCCCCCCCCCCCCCH
SEVQTLSSIDLQEVPRFSSSFKEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLA
HHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETNIDKICQVAEKEQPKIMVIDSIQ
HHCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEE
VMHVADVQSSPGSVAQVRESATALTRYAKQNNVAIFLVGHVTKDGTLAGPKVLEHIIDCS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCCE
VLLDGSADSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSV
EEECCCCCHHHHHHHHHHHHCCCHHHCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEE
MVVWEGTRPLLVEIQALVDYSQLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFV
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCEEE
NVVGGVKVAETSADLALIMALLSSFKDRPLAKDVVVFGEVGLAGEIRPVPSGQERLMEAY
EEECCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKKAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL
HHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]