Definition Vibrio fischeri ES114 chromosome I, complete genome.
Accession NC_006840
Length 2,897,536

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The map label for this gene is pnp

Identifier: 59711097

GI number: 59711097

Start: 526147

End: 528276

Strand: Direct

Name: pnp

Synonym: VF_0490

Alternate gene names: 59711097

Gene position: 526147-528276 (Clockwise)

Preceding gene: 59711096

Following gene: 59711098

Centisome position: 18.16

GC content: 43.52

Gene sequence:

>2130_bases
ATGTTCGCGAATCCAGTCGTAAAATCATTTCAGTACGGTAACCATACCGTTACTCTAGAAACGGGTGTAATTGCTCGTCA
AGCTACAGCTGCTGTAATGGCAAGCATGGACGATACATCTGTATTCGTTTCTGTTGTTGCTAAGAAAGAAGCGGTTCCAG
GCCAAGATTTCTTCCCACTAACAGTTAACTACCAAGAGCGTACGTACGCTGCGGGCAAAATCCCTGGTGGTTTCTTTAAG
CGTGAAGGTCGTCCTTCTGAAGCTGAAACACTAACAGCTCGTCTAATTGACCGTCCAATCCGTCCTCTTTTCCCTGATGC
ATTCAAAAACGAAGTACAGGTAATTGCAACGGTTGTTTCTATCAACCCTGAAGTTAACCCAGACATGATCACTATGATCG
GTACGTCTGCGGCATTAGCTATCGCTGGTATTCCATTTAATGGTCCTATCGGTGCTGCACGTGTTGGTCACATCAACGGT
GAGCTTGTTCTTAACCCATCAAATACTGAACTTGAAAACTCTAAACTAGACCTAGTTGTGTCTGGTACAGAAGGTGCAGT
ATTAATGGTTGAATCAGAAGCTGATAACCTAACTGAAGAAGAAATGCTTTCTGCTGTTGTATTCGGTCACGAACAACAAC
AAGTAGTTATCAACGCTATCAACGAATTCGCTGCTGAAGTTGCAACTCCAGCATGGGATTGGGTTGCTCCAGAAGAGAAC
ACAGTTCTTAACGCGCGTATTGCAGAGTTAGCAGAAGCTAAATTAGTTGAAGCTTACCAAATTACTGAAAAAATGACTCG
TTACGATCGCATTCATGAAATCGCAGCTGAAGTTAACGAAGTTCTAGTATCTGAAAACGAAGACGTGAACCTAAAAGAAG
TTCACACTATCTTCCACGATCTAGAGAAAACAGTTGTACGTCGCAGCATCATCGCTGGTAACCCACGTATCGATGGTCGT
GAAAAAGACATGGTTCGTGCGCTAGACGTACGTACTGGTGTTCTTCCACGTACTCACGGTTCTTCTCTATTCACACGCGG
TGAAACTCAAGCTCTTGTTACTGCAACGCTTGGTACACAACGTGATGCTCAAATCATCGATAGCCTAATGGGTGAGAAGA
AAGATCACTTCCTTCTACACTACAATTTCCCTCCATACTGTGTAGGTGAAACTGGTTTTGTTGGTTCTCCTAAGCGTCGT
GAAATCGGTCACGGTAAACTGGCTAAACGTGGTATCCAAGCGGTTATGCCTTCTATTGAAGAATTCCCATACACAGTACG
TGTAGTATCGGAAATCACTGAATCTAACGGTTCATCTTCAATGGCTTCTGTATGTGGTACATCTCTAGCTCTTATGGATG
CTGGTGTTCCAATTAAAGCATCTGTTGCTGGTATCGCAATGGGTCTTGTGAAAGAAGGTGACGATTTCGTTGTTCTTTCT
GATATTCTAGGTGACGAAGACCACCTTGGTGATATGGACTTTAAAGTAGCGGGTACTAACGAAGGTATCACTGCACTTCA
AATGGATATCAAAATCGAAGGCATCACTAAAGAGATCATGCAGATCGCTCTAAACCAAGCTCAAGGTGCGCGTAAGCACA
TCCTTAAAGTTATGGATGAAGCTATCTCTGGTGCTCGTGAAGAGATCTCTGAATTTGCTCCACGTATCCACACAATGAAG
ATCAGCTCTGATAAGATCAAAGATGTTATCGGTAAAGGTGGTGCAGTTATCCGTGCTCTTTGTGAAGAGACTGGTACAAC
TATCGAAATCGAAGATGACGGTACGATCAAAATCGCTGCAACTGAAGGCGCTGCTGCTAAAGAAGCGATTCGTCGCATCG
AAGAGATCACTGCTGAAGTTGAAGTTGGTAAGATCTACACAGGTAAAGTTATGCGTATCGTAGATTTCGGTGCGTTCGTA
ACGGTTCTTGGTCCTAAAGAAGGTCTAGTTCATATTTCTCAAATCGCAGAAGAGCGCATCGAGAAAGTAGCTGATCACCT
ACAAGTTGGTCAAGAAGTGAAAACTAAAGTACTTGAAATCGACCGTCAAGGCCGTATCCGTCTAAGTATTAAAGAAGCAA
ATGCTGAACTAAACCCAGCACCTGCTGCTGAAGCAAAAGACGCAGAATAA

Upstream 100 bases:

>100_bases
TGTACTAGTCGCGATTAGTAGTATGTTGAATTATCAACTACAAATTTCTTATTTTTGATAGCACAACGTGCCATGTAAAA
ATAAGAATAAAGGAAAAAAA

Downstream 100 bases:

>100_bases
TCTTATTGATTACAGTCTTTTGATTTAAAGCATGTTATAAAGGGAGCAGAAATGCTCCCTTTTCTTTTAATATAGAGTCA
CTTTTATTAAAAGAATTAGG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MFANPVVKSFQYGNHTVTLETGVIARQATAAVMASMDDTSVFVSVVAKKEAVPGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEAETLTARLIDRPIRPLFPDAFKNEVQVIATVVSINPEVNPDMITMIGTSAALAIAGIPFNGPIGAARVGHING
ELVLNPSNTELENSKLDLVVSGTEGAVLMVESEADNLTEEEMLSAVVFGHEQQQVVINAINEFAAEVATPAWDWVAPEEN
TVLNARIAELAEAKLVEAYQITEKMTRYDRIHEIAAEVNEVLVSENEDVNLKEVHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGKLAKRGIQAVMPSIEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
DILGDEDHLGDMDFKVAGTNEGITALQMDIKIEGITKEIMQIALNQAQGARKHILKVMDEAISGAREEISEFAPRIHTMK
ISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYTGKVMRIVDFGAFV
TVLGPKEGLVHISQIAEERIEKVADHLQVGQEVKTKVLEIDRQGRIRLSIKEANAELNPAPAAEAKDAE

Sequences:

>Translated_709_residues
MFANPVVKSFQYGNHTVTLETGVIARQATAAVMASMDDTSVFVSVVAKKEAVPGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEAETLTARLIDRPIRPLFPDAFKNEVQVIATVVSINPEVNPDMITMIGTSAALAIAGIPFNGPIGAARVGHING
ELVLNPSNTELENSKLDLVVSGTEGAVLMVESEADNLTEEEMLSAVVFGHEQQQVVINAINEFAAEVATPAWDWVAPEEN
TVLNARIAELAEAKLVEAYQITEKMTRYDRIHEIAAEVNEVLVSENEDVNLKEVHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGKLAKRGIQAVMPSIEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
DILGDEDHLGDMDFKVAGTNEGITALQMDIKIEGITKEIMQIALNQAQGARKHILKVMDEAISGAREEISEFAPRIHTMK
ISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYTGKVMRIVDFGAFV
TVLGPKEGLVHISQIAEERIEKVADHLQVGQEVKTKVLEIDRQGRIRLSIKEANAELNPAPAAEAKDAE
>Mature_709_residues
MFANPVVKSFQYGNHTVTLETGVIARQATAAVMASMDDTSVFVSVVAKKEAVPGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEAETLTARLIDRPIRPLFPDAFKNEVQVIATVVSINPEVNPDMITMIGTSAALAIAGIPFNGPIGAARVGHING
ELVLNPSNTELENSKLDLVVSGTEGAVLMVESEADNLTEEEMLSAVVFGHEQQQVVINAINEFAAEVATPAWDWVAPEEN
TVLNARIAELAEAKLVEAYQITEKMTRYDRIHEIAAEVNEVLVSENEDVNLKEVHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQRDAQIIDSLMGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGKLAKRGIQAVMPSIEEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
DILGDEDHLGDMDFKVAGTNEGITALQMDIKIEGITKEIMQIALNQAQGARKHILKVMDEAISGAREEISEFAPRIHTMK
ISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGKIYTGKVMRIVDFGAFV
TVLGPKEGLVHISQIAEERIEKVADHLQVGQEVKTKVLEIDRQGRIRLSIKEANAELNPAPAAEAKDAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=37.6420454545455, Blast_Score=442, Evalue=1e-124,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=75.1803751803752, Blast_Score=1087, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=718, Percent_Identity=31.7548746518106, Blast_Score=331, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17535281, Length=86, Percent_Identity=51.1627906976744, Blast_Score=76, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320850, Length=101, Percent_Identity=34.6534653465347, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI281362905, Length=709, Percent_Identity=38.3638928067701, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651641, Length=709, Percent_Identity=38.3638928067701, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651643, Length=709, Percent_Identity=38.3638928067701, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=38.1679389312977, Blast_Score=427, Evalue=1e-120,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_VIBF1 (Q5E7L1)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203873.1
- HSSP:   P05055
- ProteinModelPortal:   Q5E7L1
- STRING:   Q5E7L1
- GeneID:   3278250
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0490
- NMPDR:   fig|312309.3.peg.490
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q5E7L1
- ProtClustDB:   PRK11824
- BioCyc:   VFIS312309:VF0490-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76836; Mature: 76836

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MFANPVVKSFQYGNHTVTLETGVIARQATAAVMASMDDTSVFVSVVAKKEAVPGQDFFPL
CCCCHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEAETLTARLIDRPIRPLFPDAFKNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHEEEEEEEE
INPEVNPDMITMIGTSAALAIAGIPFNGPIGAARVGHINGELVLNPSNTELENSKLDLVV
CCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEE
SGTEGAVLMVESEADNLTEEEMLSAVVFGHEQQQVVINAINEFAAEVATPAWDWVAPEEN
ECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TVLNARIAELAEAKLVEAYQITEKMTRYDRIHEIAAEVNEVLVSENEDVNLKEVHTIFHD
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LEKTVVRRSIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECCCC
RDAQIIDSLMGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGKLAKRGIQAVMPSIE
CHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHH
EFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
HCCHHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCHHHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTNEGITALQMDIKIEGITKEIMQIALNQAQGARKHILKVMDE
HHCCCCCCCCCCEEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHH
AISGAREEISEFAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAA
HHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TEGAAAKEAIRRIEEITAEVEVGKIYTGKVMRIVDFGAFVTVLGPKEGLVHISQIAEERI
CCCHHHHHHHHHHHHHHHHEEECEEECCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKVADHLQVGQEVKTKVLEIDRQGRIRLSIKEANAELNPAPAAEAKDAE
HHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFANPVVKSFQYGNHTVTLETGVIARQATAAVMASMDDTSVFVSVVAKKEAVPGQDFFPL
CCCCHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEAETLTARLIDRPIRPLFPDAFKNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHEEEEEEEE
INPEVNPDMITMIGTSAALAIAGIPFNGPIGAARVGHINGELVLNPSNTELENSKLDLVV
CCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEE
SGTEGAVLMVESEADNLTEEEMLSAVVFGHEQQQVVINAINEFAAEVATPAWDWVAPEEN
ECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TVLNARIAELAEAKLVEAYQITEKMTRYDRIHEIAAEVNEVLVSENEDVNLKEVHTIFHD
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LEKTVVRRSIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECCCC
RDAQIIDSLMGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGKLAKRGIQAVMPSIE
CHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHH
EFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
HCCHHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCHHHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTNEGITALQMDIKIEGITKEIMQIALNQAQGARKHILKVMDE
HHCCCCCCCCCCEEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHH
AISGAREEISEFAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAA
HHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TEGAAAKEAIRRIEEITAEVEVGKIYTGKVMRIVDFGAFVTVLGPKEGLVHISQIAEERI
CCCHHHHHHHHHHHHHHHHEEECEEECCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKVADHLQVGQEVKTKVLEIDRQGRIRLSIKEANAELNPAPAAEAKDAE
HHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA