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The map label for this gene is ispH [H]

Identifier: 59711077

GI number: 59711077

Start: 502305

End: 503249

Strand: Direct

Name: ispH [H]

Synonym: VF_0470

Alternate gene names: 59711077

Gene position: 502305-503249 (Clockwise)

Preceding gene: 59711076

Following gene: 59711078

Centisome position: 17.34

GC content: 37.46

Gene sequence:

>945_bases
ATGAAAATAGTATTAGCTAATCCTCGTGGTTTTTGTGCCGGTGTTGATCGTGCCATTAGCATTGTTGAGCGTGCTTTAGA
GTTATATGAAGCACCTATTTATGTTCGTCATGAGGTAGTACATAATCGATTTGTTGTTGAAGGATTAAAGCAACGTGGTG
CTATTTTTGTTGAAGAATTGCACGAAGTGCCTGACGATAATATTGTTATTTTCTCTGCTCATGGTGTTTCACAAGCCGTA
CGTAAAGAAGCGAAAGAACGAGCATTAACTGTGTTTGATGCAACTTGCCCACTAGTGACAAAAGTACATATGGAAGTGGC
TCGTGCAAGTAAAAAGAATATTGAAGTTGTATTAATAGGTCATGCGGGACACCCAGAAGTTGAAGGTACAATGGGGCAGT
ATGCGAGTGATTCAGCTGGTATGTATTTAGTTGAAACTCCTGATGACGTCATTAAATTAAATGTAAAAGATCCATCGAAT
CTTCATTATGTAAGTCAAACAACATTATCTGTTGATGAAACCGCTGATGTGATTGATGAATTACGTCGAGTATTTCCTGA
AATTCAAGGACCACGTAAAGATGATATTTGTTATGCAACACAAAATCGTCAAGATGCAGTGCGTGATATGGCAAGCCAAG
TGGATGTGATGATCGTTGTTGGTTCTAAAAACTCTTCAAATTCAAATCGTTTACGCGAATTATCTGAAAAATTAGGGACA
ACGAGTTATTTAATTGATTGTCCTGAAGATCTTAAAGAAGAATGGTTAACAGAGCAGACTAAAGTTGGTGTAACTGCTGG
AGCTTCAGCCCCTGAAGAATTGGTTAACCAAATTATAGAACAAGTAAAAGCATTTGGCGGAACGGCTGTTGAAGAATTAA
CTGGTCGTGAAGAAAATATGTTTTTTGAAGTGCCAAAAGAATTGCAAATCAAAACGGTATCTTAA

Upstream 100 bases:

>100_bases
AGTGACAGTTGATTTTAATCACCCATTAGCGGGTCAAGATGTTATCTTTGATGTAGAGATTGTAGCGGTTGGTTAATCCA
ACACAATAAAGAGAAAGAAA

Downstream 100 bases:

>100_bases
TACTTCACATAGTAGTGAATAAAAATTTAAGATAGAGTGCAAGGATGCGTATTTAAAGAAGCGGAGATCAAAATGGTAAG
AGTAGCAATTGCTGGCGCAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKIVLANPRGFCAGVDRAISIVERALELYEAPIYVRHEVVHNRFVVEGLKQRGAIFVEELHEVPDDNIVIFSAHGVSQAV
RKEAKERALTVFDATCPLVTKVHMEVARASKKNIEVVLIGHAGHPEVEGTMGQYASDSAGMYLVETPDDVIKLNVKDPSN
LHYVSQTTLSVDETADVIDELRRVFPEIQGPRKDDICYATQNRQDAVRDMASQVDVMIVVGSKNSSNSNRLRELSEKLGT
TSYLIDCPEDLKEEWLTEQTKVGVTAGASAPEELVNQIIEQVKAFGGTAVEELTGREENMFFEVPKELQIKTVS

Sequences:

>Translated_314_residues
MKIVLANPRGFCAGVDRAISIVERALELYEAPIYVRHEVVHNRFVVEGLKQRGAIFVEELHEVPDDNIVIFSAHGVSQAV
RKEAKERALTVFDATCPLVTKVHMEVARASKKNIEVVLIGHAGHPEVEGTMGQYASDSAGMYLVETPDDVIKLNVKDPSN
LHYVSQTTLSVDETADVIDELRRVFPEIQGPRKDDICYATQNRQDAVRDMASQVDVMIVVGSKNSSNSNRLRELSEKLGT
TSYLIDCPEDLKEEWLTEQTKVGVTAGASAPEELVNQIIEQVKAFGGTAVEELTGREENMFFEVPKELQIKTVS
>Mature_314_residues
MKIVLANPRGFCAGVDRAISIVERALELYEAPIYVRHEVVHNRFVVEGLKQRGAIFVEELHEVPDDNIVIFSAHGVSQAV
RKEAKERALTVFDATCPLVTKVHMEVARASKKNIEVVLIGHAGHPEVEGTMGQYASDSAGMYLVETPDDVIKLNVKDPSN
LHYVSQTTLSVDETADVIDELRRVFPEIQGPRKDDICYATQNRQDAVRDMASQVDVMIVVGSKNSSNSNRLRELSEKLGT
TSYLIDCPEDLKEEWLTEQTKVGVTAGASAPEELVNQIIEQVKAFGGTAVEELTGREENMFFEVPKELQIKTVS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=67.741935483871, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34821; Mature: 34821

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLANPRGFCAGVDRAISIVERALELYEAPIYVRHEVVHNRFVVEGLKQRGAIFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
HEVPDDNIVIFSAHGVSQAVRKEAKERALTVFDATCPLVTKVHMEVARASKKNIEVVLIG
HHCCCCCEEEEECCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEE
HAGHPEVEGTMGQYASDSAGMYLVETPDDVIKLNVKDPSNLHYVSQTTLSVDETADVIDE
CCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEECEECHHHHHHHHHH
LRRVFPEIQGPRKDDICYATQNRQDAVRDMASQVDVMIVVGSKNSSNSNRLRELSEKLGT
HHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCC
TSYLIDCPEDLKEEWLTEQTKVGVTAGASAPEELVNQIIEQVKAFGGTAVEELTGREENM
CEEEEECCHHHHHHHCCHHHHCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCE
FFEVPKELQIKTVS
EEECCCCCEEEECC
>Mature Secondary Structure
MKIVLANPRGFCAGVDRAISIVERALELYEAPIYVRHEVVHNRFVVEGLKQRGAIFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
HEVPDDNIVIFSAHGVSQAVRKEAKERALTVFDATCPLVTKVHMEVARASKKNIEVVLIG
HHCCCCCEEEEECCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEE
HAGHPEVEGTMGQYASDSAGMYLVETPDDVIKLNVKDPSNLHYVSQTTLSVDETADVIDE
CCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEECEECHHHHHHHHHH
LRRVFPEIQGPRKDDICYATQNRQDAVRDMASQVDVMIVVGSKNSSNSNRLRELSEKLGT
HHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCC
TSYLIDCPEDLKEEWLTEQTKVGVTAGASAPEELVNQIIEQVKAFGGTAVEELTGREENM
CEEEEECCHHHHHHHCCHHHHCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCE
FFEVPKELQIKTVS
EEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA