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The map label for this gene is dnaB [H]

Identifier: 59710908

GI number: 59710908

Start: 309465

End: 310862

Strand: Direct

Name: dnaB [H]

Synonym: VF_0301

Alternate gene names: 59710908

Gene position: 309465-310862 (Clockwise)

Preceding gene: 59710905

Following gene: 59710909

Centisome position: 10.68

GC content: 42.42

Gene sequence:

>1398_bases
ATGGCTGATAATCGTAAGCAAAATCGCAAACCAGACGCACAAGTTGATGCGTTAAAAGTACCACCGCATTCGTTAGAAGC
TGAACAATCGGTTTTAGGTGGCTTGCTACTTGATAATGAACGCTGGGATACGGTTGCCGAAAAAGTAGTGGCAAGTGATT
TTTATAGTCGCCCACATAGACTGATTTTTGAATCACTTAAAGCCATCCTAGAAGACAGTTTGCCTTTGGATTTGATCACC
TTATCAGAGCATCTTGAACGTCATGAAAATTTAGATTCGGTAGGTGGCTTTGCTTATTTAGCTGATTTAGCTAAAAACAC
GCCAAGTGCTGCAAACATTAATGCTTATGCTGATATTGTACGTGAGCGTGCAATGGTACGTAACCTGATTGGTGTTGCGA
ATGAGATTGCAGATGCAGGTTATGATCCTCAGGGGCGAACTTCTGAAGATTTACTTGATATGGCGGAGAGTAAAGTTTTT
GCCATTGCAGAAGAGCGAACTACCGAAAATGAAGGCCCTCAAAATGTCGATTCAATTCTAGAGAAAACGTTAGAACGTAT
CGAGATTTTATACCAAACTCCGCAAGATGGGGTAACGGGGGTTTCAACTGGTTTTACTGACCTCAATAAGAAAACTGCAG
GTTTACAAGGGTCAGATTTGATTATCGTTGCTGCTCGTCCATCGATGGGTAAAACTACTTTTGCGATGAACTTATGTGAA
AACGCAGCAATGGACCAAGATAAACCCGTCCTTATCTTTTCTTTAGAGATGCCCGCAGAACAGCTAATGATGCGTATGTT
GGCTTCTCTTTCTCGTGTAGACCAAACTAAAATTCGTACCGGCCAGCTTGATGACGAAGATTGGGCTCGTATTTCTTCTA
CTATGGGCATTTTGACTCAGAAGAAAAACATGTATATCGATGATAGCTCAGGCTTAACACCGACAGAATTACGTTCACGT
GCTCGTCGTGTTGCGCGTGAATCGGGTGGTCTAAGTATGATCATGGTCGATTACCTTCAATTAATGCGTGTACCTGGCTT
GCAAGATAACCGTACGTTAGAGATCTCTGAAATATCACGATCGTTAAAAGCGTTAGCGAAAGAGTTGAACGTTCCTGTTG
TGGCGCTGTCGCAACTTAACCGTTCCCTAGAACAACGTGCAGATAAACGTCCGATTAACTCGGATTTGCGTGAATCGGGT
GCGATCGAGCAGGATGCCGATTTAATCATGTTTATTTACCGTGATGAGGTATATAACCCTGAGAGTAATCGTAAAGGTAT
CGCTGAAATTATTATTGGTAAGCAACGTAACGGTCCTATCGGTTCTGTTAGCTTGACTTTCCAAGGGCAATTTTCTCGTT
TTGATAATTACGCAGGCCCTGCGTTTGATGATGAATAA

Upstream 100 bases:

>100_bases
ATTTTGCGAAAGGAGTGATGGAGATGGAAGGATAGTTATTTCATCCCTTTGCCAAATGGTTATAATGTAGGGCTAAATTT
AGGATGGTGAAAGAGTAGTA

Downstream 100 bases:

>100_bases
TAAGGCTGAATCATGAGTTATATGAAAGCGGCCACAGCGCAGATTGATATGTCTGCACTTGCCCACAATTTGCAAAAGAT
TAAACAACAAGCTCCGAGTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MADNRKQNRKPDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVASDFYSRPHRLIFESLKAILEDSLPLDLIT
LSEHLERHENLDSVGGFAYLADLAKNTPSAANINAYADIVRERAMVRNLIGVANEIADAGYDPQGRTSEDLLDMAESKVF
AIAEERTTENEGPQNVDSILEKTLERIEILYQTPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
NAAMDQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILTQKKNMYIDDSSGLTPTELRSR
ARRVARESGGLSMIMVDYLQLMRVPGLQDNRTLEISEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESG
AIEQDADLIMFIYRDEVYNPESNRKGIAEIIIGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE

Sequences:

>Translated_465_residues
MADNRKQNRKPDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVASDFYSRPHRLIFESLKAILEDSLPLDLIT
LSEHLERHENLDSVGGFAYLADLAKNTPSAANINAYADIVRERAMVRNLIGVANEIADAGYDPQGRTSEDLLDMAESKVF
AIAEERTTENEGPQNVDSILEKTLERIEILYQTPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
NAAMDQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILTQKKNMYIDDSSGLTPTELRSR
ARRVARESGGLSMIMVDYLQLMRVPGLQDNRTLEISEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESG
AIEQDADLIMFIYRDEVYNPESNRKGIAEIIIGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE
>Mature_464_residues
ADNRKQNRKPDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVASDFYSRPHRLIFESLKAILEDSLPLDLITL
SEHLERHENLDSVGGFAYLADLAKNTPSAANINAYADIVRERAMVRNLIGVANEIADAGYDPQGRTSEDLLDMAESKVFA
IAEERTTENEGPQNVDSILEKTLERIEILYQTPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCEN
AAMDQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILTQKKNMYIDDSSGLTPTELRSRA
RRVARESGGLSMIMVDYLQLMRVPGLQDNRTLEISEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESGA
IEQDADLIMFIYRDEVYNPESNRKGIAEIIIGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=77.9193205944798, Blast_Score=751, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51669; Mature: 51538

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNRKQNRKPDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVASDFYSRPHR
CCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCHHH
LIFESLKAILEDSLPLDLITLSEHLERHENLDSVGGFAYLADLAKNTPSAANINAYADIV
HHHHHHHHHHHCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RERAMVRNLIGVANEIADAGYDPQGRTSEDLLDMAESKVFAIAEERTTENEGPQNVDSIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEHHHCCCCCCCCHHHHHH
EKTLERIEILYQTPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
HHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
NAAMDQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILTQ
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEE
KKNMYIDDSSGLTPTELRSRARRVARESGGLSMIMVDYLQLMRVPGLQDNRTLEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHH
SLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESGAIEQDADLIMFIYRDEVYNP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCC
ESNRKGIAEIIIGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE
CCCCCCHHHHEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADNRKQNRKPDAQVDALKVPPHSLEAEQSVLGGLLLDNERWDTVAEKVVASDFYSRPHR
CCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCHHH
LIFESLKAILEDSLPLDLITLSEHLERHENLDSVGGFAYLADLAKNTPSAANINAYADIV
HHHHHHHHHHHCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RERAMVRNLIGVANEIADAGYDPQGRTSEDLLDMAESKVFAIAEERTTENEGPQNVDSIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEHHHCCCCCCCCHHHHHH
EKTLERIEILYQTPQDGVTGVSTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCE
HHHHHHHHHHHCCCCCCCCCCCCCCHHCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
NAAMDQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILTQ
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEE
KKNMYIDDSSGLTPTELRSRARRVARESGGLSMIMVDYLQLMRVPGLQDNRTLEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHH
SLKALAKELNVPVVALSQLNRSLEQRADKRPINSDLRESGAIEQDADLIMFIYRDEVYNP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCC
ESNRKGIAEIIIGKQRNGPIGSVSLTFQGQFSRFDNYAGPAFDDE
CCCCCCHHHHEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]