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The map label for this gene is ksgA [H]

Identifier: 59710893

GI number: 59710893

Start: 290404

End: 291222

Strand: Reverse

Name: ksgA [H]

Synonym: VF_0286

Alternate gene names: 59710893

Gene position: 291222-290404 (Counterclockwise)

Preceding gene: 59710894

Following gene: 59710892

Centisome position: 10.05

GC content: 41.15

Gene sequence:

>819_bases
ATGAGTACAAGAAATGATGTCCATTTAGGCCATAAAGCAAGAAAACGCTTTGGTCAAAACTTCTTAAATGACCCTTATGT
GATCGATGGAATCGTATCTGCAATCAACCCTCTACCAGGCCAAAACCTTGTAGAAATTGGTCCAGGTCTTGGTGCTATCA
CTGAGCCGGTTGGACGTGAAGTAGATAAATTTACGGTTATTGAACTTGACCGTGACTTAGCTGAACGTTTACGTAACCAC
CCTGAATTGGGCAGTAAGCTAACAATTCATGAAGGCGATGCAATGCGCTTTGACTTCACTCAGCTAATCAAAGAAAACAA
TAAACTGCGTATTTTTGGTAACCTACCATACAATATTTCTACGCCGTTAATGTTCCACCTTTTTGAATTCCATAAAGACG
TACAAGACATGCACTTTATGCTACAAAAAGAAGTGGTTAATCGTCTAGCTGCTGGTCCTGGTACAAAAGCATATGGTCGT
TTAACGGTTATGGCTCAATACTTCTGTAAGGTTATGCCTGTACTAGAAGTGCCACCAACAGCATTTGTTCCGCCACCGAA
GGTTGATTCTGCGGTTGTTCGTCTTGTTCCTTACGAAACGCTGCCTTACCCAGCAACAAACTTAAAATGGCTAGACCGTG
TATGTCGTGAAGGTTTTAACCAACGTCGTAAGACAGTCCGTAACTGTTATAAAGCATTGCTCACTAAAGAGCAGTTGGAA
GAATTAGGTATTAATCCTTCTATGCGCCCTGAAAACCTAACATTAGAGCAGTTTGTTAATATGGCGAACTGGTTAGATGC
AAATCATAGTGCAGAGTAA

Upstream 100 bases:

>100_bases
TAGACCATGGTACTGCATTGGAATTAGCAGGTACTGGAAAAGCAGATGCAGGAAGCTTTATTACCGCACTTACCCATGCA
ATAGAACTGGTAGAAAATAA

Downstream 100 bases:

>100_bases
GCATTAACTGATTTCAATTGAGGTGACCTTATGGTCACCTTTTTTATATTTATCATATTTTGAGAGAATGGTATGGCAAC
TTACTTCGTTGGTGATATTC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSTRNDVHLGHKARKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNH
PELGSKLTIHEGDAMRFDFTQLIKENNKLRIFGNLPYNISTPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGR
LTVMAQYFCKVMPVLEVPPTAFVPPPKVDSAVVRLVPYETLPYPATNLKWLDRVCREGFNQRRKTVRNCYKALLTKEQLE
ELGINPSMRPENLTLEQFVNMANWLDANHSAE

Sequences:

>Translated_272_residues
MSTRNDVHLGHKARKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNH
PELGSKLTIHEGDAMRFDFTQLIKENNKLRIFGNLPYNISTPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGR
LTVMAQYFCKVMPVLEVPPTAFVPPPKVDSAVVRLVPYETLPYPATNLKWLDRVCREGFNQRRKTVRNCYKALLTKEQLE
ELGINPSMRPENLTLEQFVNMANWLDANHSAE
>Mature_271_residues
STRNDVHLGHKARKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNHP
ELGSKLTIHEGDAMRFDFTQLIKENNKLRIFGNLPYNISTPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRL
TVMAQYFCKVMPVLEVPPTAFVPPPKVDSAVVRLVPYETLPYPATNLKWLDRVCREGFNQRRKTVRNCYKALLTKEQLEE
LGINPSMRPENLTLEQFVNMANWLDANHSAE

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=32.258064516129, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI7657198, Length=229, Percent_Identity=31.4410480349345, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=65.2830188679245, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25141369, Length=294, Percent_Identity=27.891156462585, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=230, Percent_Identity=33.4782608695652, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=234, Percent_Identity=30.3418803418803, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=33.2129963898917, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21358017, Length=246, Percent_Identity=32.520325203252, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31023; Mature: 30892

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRNDVHLGHKARKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGRE
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHCCC
VDKFTVIELDRDLAERLRNHPELGSKLTIHEGDAMRFDFTQLIKENNKLRIFGNLPYNIS
CHHEEEEECCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
TPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYFCKVMPVLEVPPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AFVPPPKVDSAVVRLVPYETLPYPATNLKWLDRVCREGFNQRRKTVRNCYKALLTKEQLE
CCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGINPSMRPENLTLEQFVNMANWLDANHSAE
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STRNDVHLGHKARKRFGQNFLNDPYVIDGIVSAINPLPGQNLVEIGPGLGAITEPVGRE
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHCCC
VDKFTVIELDRDLAERLRNHPELGSKLTIHEGDAMRFDFTQLIKENNKLRIFGNLPYNIS
CHHEEEEECCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
TPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYFCKVMPVLEVPPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AFVPPPKVDSAVVRLVPYETLPYPATNLKWLDRVCREGFNQRRKTVRNCYKALLTKEQLE
CCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGINPSMRPENLTLEQFVNMANWLDANHSAE
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA