Click here to switch to the map view.

The map label for this gene is folA [H]

Identifier: 59710891

GI number: 59710891

Start: 288794

End: 289261

Strand: Direct

Name: folA [H]

Synonym: VF_0284

Alternate gene names: 59710891

Gene position: 288794-289261 (Clockwise)

Preceding gene: 59710890

Following gene: 59710897

Centisome position: 9.97

GC content: 39.53

Gene sequence:

>468_bases
ATGATAGCCGCAATGGCAAAGAATCGTATTATTGGTATTGATAATCAGATGCCATGGCATTTACCCGCTGATTTCGCTTG
GTTTAAAAAATGCACTATGGGTAAACCTATTGTCATGGGGCGTAAGACTTATGAATCTATTGGTCGTCCATTACCGGGCC
GATTGAATATCGTATTAAGCCGTGATGCTAATTTACAAATAGACGGAGTGACTTGTGTTACTTCGATTGAAGAAGCGAAA
CAAGCTGCAGGTGATATTGAAGAACTTATGATTATTGGTGGTGGCTCAATTTACCAAGCGTGTCTTTCTGATGCAGATAA
GTTATACCTGACTTTTATTGATGCTGACATCAATGGCGATACTCAATTCCCTGATTGGGGTGAGGGTTGGAATCAGACTC
ATTCTGAAACGTATTCGAAAGATGAAAAGAATCAGTATGATATGGAGTTTGTGGTTCTTGAGAGATAA

Upstream 100 bases:

>100_bases
GCCGCATTGGCAATTGGCCTTGCTATGCCCGGGTTACTGTTTTACCGTAGAAAGTCGATTGTTTAAGTATAGTGATTAAG
GAATAACTGTGAAAATTAGC

Downstream 100 bases:

>100_bases
GAGCAGGACGAACTTCGTTCTAAAGGGCGCTTCGCTTAAGGAGGCTGCGCCCTGAGGAATAAAAGCAAAGAGAAAAACGG
AATACAGATTACTATCCTTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MIAAMAKNRIIGIDNQMPWHLPADFAWFKKCTMGKPIVMGRKTYESIGRPLPGRLNIVLSRDANLQIDGVTCVTSIEEAK
QAAGDIEELMIIGGGSIYQACLSDADKLYLTFIDADINGDTQFPDWGEGWNQTHSETYSKDEKNQYDMEFVVLER

Sequences:

>Translated_155_residues
MIAAMAKNRIIGIDNQMPWHLPADFAWFKKCTMGKPIVMGRKTYESIGRPLPGRLNIVLSRDANLQIDGVTCVTSIEEAK
QAAGDIEELMIIGGGSIYQACLSDADKLYLTFIDADINGDTQFPDWGEGWNQTHSETYSKDEKNQYDMEFVVLER
>Mature_155_residues
MIAAMAKNRIIGIDNQMPWHLPADFAWFKKCTMGKPIVMGRKTYESIGRPLPGRLNIVLSRDANLQIDGVTCVTSIEEAK
QAAGDIEELMIIGGGSIYQACLSDADKLYLTFIDADINGDTQFPDWGEGWNQTHSETYSKDEKNQYDMEFVVLER

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=144, Percent_Identity=31.9444444444444, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1786233, Length=156, Percent_Identity=52.5641025641026, Blast_Score=172, Evalue=9e-45,
Organism=Drosophila melanogaster, GI116008026, Length=176, Percent_Identity=28.9772727272727, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24647458, Length=176, Percent_Identity=28.9772727272727, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17410; Mature: 17410

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAAMAKNRIIGIDNQMPWHLPADFAWFKKCTMGKPIVMGRKTYESIGRPLPGRLNIVLS
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCEEECCHHHHHHCCCCCCCEEEEEE
RDANLQIDGVTCVTSIEEAKQAAGDIEELMIIGGGSIYQACLSDADKLYLTFIDADINGD
CCCCEEECCEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEEEEEECCCCCC
TQFPDWGEGWNQTHSETYSKDEKNQYDMEFVVLER
CCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MIAAMAKNRIIGIDNQMPWHLPADFAWFKKCTMGKPIVMGRKTYESIGRPLPGRLNIVLS
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCEEECCHHHHHHCCCCCCCEEEEEE
RDANLQIDGVTCVTSIEEAKQAAGDIEELMIIGGGSIYQACLSDADKLYLTFIDADINGD
CCCCEEECCEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEEEEEECCCCCC
TQFPDWGEGWNQTHSETYSKDEKNQYDMEFVVLER
CCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]