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The map label for this gene is ispB [H]

Identifier: 59710884

GI number: 59710884

Start: 284128

End: 285099

Strand: Reverse

Name: ispB [H]

Synonym: VF_0277

Alternate gene names: 59710884

Gene position: 285099-284128 (Counterclockwise)

Preceding gene: 59710892

Following gene: 59710882

Centisome position: 9.84

GC content: 40.64

Gene sequence:

>972_bases
ATGGATTTTAAAGCTATTCAAGCCCTAACGGCTGATGACATGGCAATCGTCAATGAGACCATATTAGCCCAACTCAACTC
AGATGTTGCGTTAATCAACCAACTTGGTTTTTACATTGTAAGCGGCGGTGGAAAACGTCTACGCCCAGTTCTTGCTATTT
TATCTGCCAAAGCACTTGGATACGAAGGTAAAGACCATACAATGGCTGCAGCTTTCATTGAGTTTATTCATACCGCAACC
CTGTTGCATGATGACGTTGTTGATGAATCAGACATGCGCCGTGGTAAAGCAACAGCAAATGCAGAGTTCGGAAATGCAGC
AAGTGTACTTGTTGGTGATTTCATCTATACCCGCTCTTTCCAAATGATGACAAGCTTAGGATCCATAAAGATCCTTAGTT
TAATGAGTGAAGCCGTAAATGTGATCGCCGAAGGTGAAGTTCTTCAATTAATGAACTGTAACGATCCTAATACGACTGAA
GAAAGTTATATGCAAGTGATCTACTCTAAAACAGCTCGTTTATTTGAAGCCGCAACGCAGATCGGTGCGATCCTAAACGA
TGCCCCACAAGAAATTGAAACGGCATTACAGAATTACGGAAAGTATTTAGGTACCGCCTTCCAACTTATTGATGATGTCA
TGGATTACACCTCAGATGGTAAAGACATGGGTAAAAATGTTGGCGATGACCTTGCTGAAGGTAAACCAACTCTTCCTCTA
TTACATGCAATGATGCACGGAAATGAAGAACAAAATGCAATGATCCGTGAAGCAATCGAAAATGGTAATGGTTTAGATAA
ACTTGAGCCGATCCTTGCTTGTATGAAAGAAGTTGGCTCTTTAGAGTACACAAAACAAAAAGCAGATGAAGAAGCAGATA
AAGCGATTGCTGAATTAGCCATTCTTCCTGAATCAGATTATAAAGAAGCACTAAAAGCATTAGCGCACCTAGCCGTTAAA
CGCAGTAAATAA

Upstream 100 bases:

>100_bases
CAATTTTCGACTGTATTGCTTAGCCCATTTTAGTGTATTATTTAGTTAATCACTTATTATAATTTAGCCTGACTTAGGCC
CTAACCTTGCTGGAAGAATA

Downstream 100 bases:

>100_bases
TCCACACCTTTTCTTTCAAACATAATAAAGGGGCAGATAGTTAGACTATCTGCCCCTTTATTCTTTCAATTAATGTCTAA
GTTAACACTTATTGAACAAA

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALGYEGKDHTMAAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSFQMMTSLGSIKILSLMSEAVNVIAEGEVLQLMNCNDPNTTE
ESYMQVIYSKTARLFEAATQIGAILNDAPQEIETALQNYGKYLGTAFQLIDDVMDYTSDGKDMGKNVGDDLAEGKPTLPL
LHAMMHGNEEQNAMIREAIENGNGLDKLEPILACMKEVGSLEYTKQKADEEADKAIAELAILPESDYKEALKALAHLAVK
RSK

Sequences:

>Translated_323_residues
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALGYEGKDHTMAAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSFQMMTSLGSIKILSLMSEAVNVIAEGEVLQLMNCNDPNTTE
ESYMQVIYSKTARLFEAATQIGAILNDAPQEIETALQNYGKYLGTAFQLIDDVMDYTSDGKDMGKNVGDDLAEGKPTLPL
LHAMMHGNEEQNAMIREAIENGNGLDKLEPILACMKEVGSLEYTKQKADEEADKAIAELAILPESDYKEALKALAHLAVK
RSK
>Mature_323_residues
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALGYEGKDHTMAAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSFQMMTSLGSIKILSLMSEAVNVIAEGEVLQLMNCNDPNTTE
ESYMQVIYSKTARLFEAATQIGAILNDAPQEIETALQNYGKYLGTAFQLIDDVMDYTSDGKDMGKNVGDDLAEGKPTLPL
LHAMMHGNEEQNAMIREAIENGNGLDKLEPILACMKEVGSLEYTKQKADEEADKAIAELAILPESDYKEALKALAHLAVK
RSK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=31.055900621118, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI4758430, Length=265, Percent_Identity=25.2830188679245, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI83700220, Length=265, Percent_Identity=25.2830188679245, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=71.2074303405573, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1786623, Length=232, Percent_Identity=28.448275862069, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=331, Percent_Identity=31.1178247734139, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=32.4414715719064, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6325188, Length=288, Percent_Identity=23.2638888888889, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651612, Length=300, Percent_Identity=31.3333333333333, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI281365769, Length=278, Percent_Identity=22.3021582733813, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24660002, Length=278, Percent_Identity=22.3021582733813, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35149; Mature: 35149

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHC
YEGKDHTMAAAFIEFIHTATLLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QMMTSLGSIKILSLMSEAVNVIAEGEVLQLMNCNDPNTTEESYMQVIYSKTARLFEAATQ
HHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAILNDAPQEIETALQNYGKYLGTAFQLIDDVMDYTSDGKDMGKNVGDDLAEGKPTLPL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCHHH
LHAMMHGNEEQNAMIREAIENGNGLDKLEPILACMKEVGSLEYTKQKADEEADKAIAELA
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ILPESDYKEALKALAHLAVKRSK
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHC
YEGKDHTMAAAFIEFIHTATLLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QMMTSLGSIKILSLMSEAVNVIAEGEVLQLMNCNDPNTTEESYMQVIYSKTARLFEAATQ
HHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAILNDAPQEIETALQNYGKYLGTAFQLIDDVMDYTSDGKDMGKNVGDDLAEGKPTLPL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCHHH
LHAMMHGNEEQNAMIREAIENGNGLDKLEPILACMKEVGSLEYTKQKADEEADKAIAELA
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ILPESDYKEALKALAHLAVKRSK
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]