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The map label for this gene is rfaD

Identifier: 59710759

GI number: 59710759

Start: 170815

End: 171756

Strand: Reverse

Name: rfaD

Synonym: VF_0152

Alternate gene names: 59710759

Gene position: 171756-170815 (Counterclockwise)

Preceding gene: 172087642

Following gene: 59710758

Centisome position: 5.93

GC content: 40.55

Gene sequence:

>942_bases
ATGATCATTGTAACTGGCGGCGCTGGCATGATTGGCAGCAACATTGTTAAGTCTTTAAATAACAAAGGTTTTAACGACAT
TTTAGTGGTTGATAACCTAAAAGATGGTAAAAAATTCAAGAACCTAGTGGATCTTGATATCACTGATTACATGGATAAAG
AAGACTTCATTACGCAAATTATGGCGGGTGATGACTTTGGTCCTATCGAAGCTATTTTCCATGAAGGTGCTTGCTCTGCT
ACAACTGAGTGGGATGGCAAATACATTATGATGAACAACTATGAGTACTCTAAAGAGTTGCTTCATTTCTGTATTGAGCG
TGAAATTCCATTCCTTTACGCATCATCAGCAGCTACTTACGGTGAAACAGATACGTTTATCGAAGAGCGTGCATACGAAG
GTGCTTTAAACGTTTACGGTTATTCTAAACAACAGTTTGATAACTACGTTCGTCGCCTATGGGCTGATGCTGAAGAGCAC
AACGAAACGCTATCTCAAATTACGGGTTTCCGTTACTTCAATGTGTATGGTCCTCGTGAACAACATAAAGGTTCTATGGC
TTCAGTTGCTTTCCATTTAAATAACCAAATGAATGCGGGCGATAACCCTAAACTGTTTGAAGGTAGTGACGAGTTTAAAC
GTGATTTCGTTTACGTTGGTGATGTTGCTGCAGTTAACCTTTGGTTCTTAGAAAATGGCGTATCTGGTATTTATAACTGT
GGTACTGGCCGTGCTGAACCATTCCGCGCTGTTGCTGAAGCGGTAATTAAACACCACGGTAAAGGTGAAGTGGAAACTAT
CCCATTCCCTGAGCACCTAAAAGGCGCATACCAAGAATTTACTCAAGCTGATCTAACTAAGCTTCGTGCTGCTGGTTGTG
ATGTTGAGTTTAAGTCAGTAGCTGATGGTGTTGCTGAATATATGGCGATGATTAATAAGTAG

Upstream 100 bases:

>100_bases
CTAAGTGTTATCAACTCTCGCCCCTGCTTTTCAAGCGTAGCGCCACCTCTTCTTCTTTGCTACAATTCAATCAATTATAT
TATTTTTCGGTGGTATCAAA

Downstream 100 bases:

>100_bases
ATTTAAATCAGAAATAGAAGCAGTACAATGATAAACAATTATTGCCAAAAAATCTTATCCAGTATAAGTAAATATTGGGT
TCTAGCTATAGTTTATATAC

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIIVTGGAGMIGSNIVKSLNNKGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQIMAGDDFGPIEAIFHEGACSA
TTEWDGKYIMMNNYEYSKELLHFCIEREIPFLYASSAATYGETDTFIEERAYEGALNVYGYSKQQFDNYVRRLWADAEEH
NETLSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNAGDNPKLFEGSDEFKRDFVYVGDVAAVNLWFLENGVSGIYNC
GTGRAEPFRAVAEAVIKHHGKGEVETIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSVADGVAEYMAMINK

Sequences:

>Translated_313_residues
MIIVTGGAGMIGSNIVKSLNNKGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQIMAGDDFGPIEAIFHEGACSA
TTEWDGKYIMMNNYEYSKELLHFCIEREIPFLYASSAATYGETDTFIEERAYEGALNVYGYSKQQFDNYVRRLWADAEEH
NETLSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNAGDNPKLFEGSDEFKRDFVYVGDVAAVNLWFLENGVSGIYNC
GTGRAEPFRAVAEAVIKHHGKGEVETIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSVADGVAEYMAMINK
>Mature_313_residues
MIIVTGGAGMIGSNIVKSLNNKGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQIMAGDDFGPIEAIFHEGACSA
TTEWDGKYIMMNNYEYSKELLHFCIEREIPFLYASSAATYGETDTFIEERAYEGALNVYGYSKQQFDNYVRRLWADAEEH
NETLSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNAGDNPKLFEGSDEFKRDFVYVGDVAAVNLWFLENGVSGIYNC
GTGRAEPFRAVAEAVIKHHGKGEVETIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSVADGVAEYMAMINK

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=314, Percent_Identity=74.2038216560509, Blast_Score=479, Evalue=1e-136,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_VIBF1 (Q5E8J9)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203535.1
- HSSP:   P67910
- ProteinModelPortal:   Q5E8J9
- SMR:   Q5E8J9
- STRING:   Q5E8J9
- GeneID:   3277911
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0152
- NMPDR:   fig|312309.3.peg.151
- eggNOG:   COG0451
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- PhylomeDB:   Q5E8J9
- ProtClustDB:   PRK11150
- BioCyc:   VFIS312309:VF0152-MONOMER
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 35145; Mature: 35145

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: ACT_SITE 139-139 ACT_SITE 183-183 BINDING 38-38 BINDING 53-53 BINDING 92-92 BINDING 143-143 BINDING 174-174 BINDING 175-175 BINDING 183-183 BINDING 185-185 BINDING 192-192 BINDING 214-214 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGMIGSNIVKSLNNKGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQI
CEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCHHHHHHHH
MAGDDFGPIEAIFHEGACSATTEWDGKYIMMNNYEYSKELLHFCIEREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCC
GETDTFIEERAYEGALNVYGYSKQQFDNYVRRLWADAEEHNETLSQITGFRYFNVYGPRE
CCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCHH
QHKGSMASVAFHLNNQMNAGDNPKLFEGSDEFKRDFVYVGDVAAVNLWFLENGVSGIYNC
HHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCEEEECCHHEEEEEEECCCCCCEEEC
GTGRAEPFRAVAEAVIKHHGKGEVETIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSV
CCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ADGVAEYMAMINK
HHHHHHHHHHHCC
>Mature Secondary Structure
MIIVTGGAGMIGSNIVKSLNNKGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQI
CEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCHHHHHHHH
MAGDDFGPIEAIFHEGACSATTEWDGKYIMMNNYEYSKELLHFCIEREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCC
GETDTFIEERAYEGALNVYGYSKQQFDNYVRRLWADAEEHNETLSQITGFRYFNVYGPRE
CCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCHH
QHKGSMASVAFHLNNQMNAGDNPKLFEGSDEFKRDFVYVGDVAAVNLWFLENGVSGIYNC
HHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCEEEECCHHEEEEEEECCCCCCEEEC
GTGRAEPFRAVAEAVIKHHGKGEVETIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSV
CCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ADGVAEYMAMINK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA