Click here to switch to the map view.

The map label for this gene is dfp

Identifier: 59710732

GI number: 59710732

Start: 143127

End: 144356

Strand: Reverse

Name: dfp

Synonym: VF_0125

Alternate gene names: 59710732

Gene position: 144356-143127 (Counterclockwise)

Preceding gene: 59710739

Following gene: 172087641

Centisome position: 4.98

GC content: 44.31

Gene sequence:

>1230_bases
ATGGCATCTCTTGCAGGGAAACGAATTCTTCTTGGTATTAGCGGCGGCATTGCAGCTTATAAATGTGCAGAACTGACTCG
TCGATTGATTGAACGCGGCGCTGAAGTTCGTGTAGTAATGACAAAAGCAGCAAAAGAGTTCATTACCCCATTAACAATGC
AGGCGGTTTCTGGCCACCCTGTTGCTGATAGTTTACTTGATCCTGCTGCTGAAGCATCAATGGGACACATTGAGTTAGCA
AAGTGGGCTGACATTGTTTTACTTGCTCCTGCTACGGCTGATCTGATTGCTCGAATGGCTGCTGGCATGGGTAACGATTT
ATTATCTACTTTAGTTCTTGCTACAGATTCTCCTGTCGCGGTCTCTCCTGCTATGAATCAGCAAATGTATAGCAATATCG
CGACGCAAGAAAACATAGCAACACTAGCTCGTCGTGGCATGCACATTTGGGGCCCAGCAGCGGGCCAACAAGCTTGTGGT
GATGTGGGCATGGGAAGAATGCTTGAGCCAATGGAGCTGGTTCATTTATGTGAAGACTTTTTCCAAGCGAATTCTTGTGA
ACAAGCAGAAGCTGATGAAAAACCACTTAAAGGTCAATCTCTACTTATTACTGCGGGCCCAACTCGTGAAGCCATCGATC
CTGTTCGTTATATCTCTAACCATAGCTCAGGGAAAATGGGATTTGCTATTGCAGAAGCCGCAGCTCAATTAGGTGCTAAC
GTCACTTTAGTCAGTGGTCCTGTTAACCTTGCAACACCAGAGAACGTGAAACGCATTGATATTGAAAGCGCAGAACAAAT
GCACGGAGCGGTAATGAAAAATGCACAATCTCATTCTATCTTTATCGCATGTGCTGCCGTTGCTGATTTCAAACTAAGCC
AAGTGGCGACTCAAAAATTAAAGAAAACCGCCGATGAAGATGGCATGACGCTTCATATGGTTAAGAACCCAGATATTGTC
GCTTCAGTTGCAGCATTAACGAATCAGCGTCCATTTACTGTTGGTTTTGCAGCTGAAACTCAAGATGTGGAAAAATACGC
TCGCGGTAAATTAGAACGCAAAAATTTAGATATGATCTGTGCAAACGATGTATCAGTTCAGGGACAAGGCTTTAATAGTG
ATAACAATGCGCTTCACCTATATTGGAAAGAAGGTGATAAAGCGCTACCGTTAACGACGAAGTCGGAGTTGGGTAAAGCG
ATTATGCTTGAGATCGTTGAGAAACTGTAG

Upstream 100 bases:

>100_bases
TTTACTTGATGGAAGAGATTGGAAGTGCATCACTCTGAAAAAAAATTATGATATGGTTAGCGCTACTTTATGGTGAACCA
CTAACAGGGTAATTAGTGTC

Downstream 100 bases:

>100_bases
AAGCAGGATTCAGATCGGTCGCAAGCTCCCTTACAGGGTTCAGAGGTGCTCGCTGATATTTCAACGCTCCCCTCTGAATT
CTGCAAGCGAAGCGATCTGA

Product: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MASLAGKRILLGISGGIAAYKCAELTRRLIERGAEVRVVMTKAAKEFITPLTMQAVSGHPVADSLLDPAAEASMGHIELA
KWADIVLLAPATADLIARMAAGMGNDLLSTLVLATDSPVAVSPAMNQQMYSNIATQENIATLARRGMHIWGPAAGQQACG
DVGMGRMLEPMELVHLCEDFFQANSCEQAEADEKPLKGQSLLITAGPTREAIDPVRYISNHSSGKMGFAIAEAAAQLGAN
VTLVSGPVNLATPENVKRIDIESAEQMHGAVMKNAQSHSIFIACAAVADFKLSQVATQKLKKTADEDGMTLHMVKNPDIV
ASVAALTNQRPFTVGFAAETQDVEKYARGKLERKNLDMICANDVSVQGQGFNSDNNALHLYWKEGDKALPLTTKSELGKA
IMLEIVEKL

Sequences:

>Translated_409_residues
MASLAGKRILLGISGGIAAYKCAELTRRLIERGAEVRVVMTKAAKEFITPLTMQAVSGHPVADSLLDPAAEASMGHIELA
KWADIVLLAPATADLIARMAAGMGNDLLSTLVLATDSPVAVSPAMNQQMYSNIATQENIATLARRGMHIWGPAAGQQACG
DVGMGRMLEPMELVHLCEDFFQANSCEQAEADEKPLKGQSLLITAGPTREAIDPVRYISNHSSGKMGFAIAEAAAQLGAN
VTLVSGPVNLATPENVKRIDIESAEQMHGAVMKNAQSHSIFIACAAVADFKLSQVATQKLKKTADEDGMTLHMVKNPDIV
ASVAALTNQRPFTVGFAAETQDVEKYARGKLERKNLDMICANDVSVQGQGFNSDNNALHLYWKEGDKALPLTTKSELGKA
IMLEIVEKL
>Mature_408_residues
ASLAGKRILLGISGGIAAYKCAELTRRLIERGAEVRVVMTKAAKEFITPLTMQAVSGHPVADSLLDPAAEASMGHIELAK
WADIVLLAPATADLIARMAAGMGNDLLSTLVLATDSPVAVSPAMNQQMYSNIATQENIATLARRGMHIWGPAAGQQACGD
VGMGRMLEPMELVHLCEDFFQANSCEQAEADEKPLKGQSLLITAGPTREAIDPVRYISNHSSGKMGFAIAEAAAQLGANV
TLVSGPVNLATPENVKRIDIESAEQMHGAVMKNAQSHSIFIACAAVADFKLSQVATQKLKKTADEDGMTLHMVKNPDIVA
SVAALTNQRPFTVGFAAETQDVEKYARGKLERKNLDMICANDVSVQGQGFNSDNNALHLYWKEGDKALPLTTKSELGKAI
MLEIVEKL

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family

Homologues:

Organism=Homo sapiens, GI71725351, Length=187, Percent_Identity=31.5508021390374, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI87082301, Length=407, Percent_Identity=64.6191646191646, Blast_Score=516, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17560194, Length=175, Percent_Identity=29.7142857142857, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322762, Length=189, Percent_Identity=31.2169312169312, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24657262, Length=186, Percent_Identity=29.0322580645161, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COABC_VIBF1 (Q5E8M6)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203508.1
- ProteinModelPortal:   Q5E8M6
- STRING:   Q5E8M6
- GeneID:   3277912
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0125
- NMPDR:   fig|312309.3.peg.124
- eggNOG:   COG0452
- HOGENOM:   HBG523890
- OMA:   VTSGPTH
- PhylomeDB:   Q5E8M6
- ProtClustDB:   PRK05579
- BioCyc:   VFIS312309:VF0125-MONOMER
- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382
- Gene3D:   G3DSA:3.40.50.10300
- Gene3D:   G3DSA:3.40.50.1950
- TIGRFAMs:   TIGR00521

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein; SSF102645 DNA/pantothenate-metab_flavo_C; SSF52507 Flavoprotein

EC number: =4.1.1.36; =6.3.2.5

Molecular weight: Translated: 43622; Mature: 43491

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: ACT_SITE 159-159 BINDING 126-126 BINDING 289-289 BINDING 299-299 BINDING 350-350 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLAGKRILLGISGGIAAYKCAELTRRLIERGAEVRVVMTKAAKEFITPLTMQAVSGHP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHCCCCC
VADSLLDPAAEASMGHIELAKWADIVLLAPATADLIARMAAGMGNDLLSTLVLATDSPVA
CHHHHHCCHHHCCCCCEEHHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
VSPAMNQQMYSNIATQENIATLARRGMHIWGPAAGQQACGDVGMGRMLEPMELVHLCEDF
ECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHH
FQANSCEQAEADEKPLKGQSLLITAGPTREAIDPVRYISNHSSGKMGFAIAEAAAQLGAN
HCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
VTLVSGPVNLATPENVKRIDIESAEQMHGAVMKNAQSHSIFIACAAVADFKLSQVATQKL
EEEEECCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHH
KKTADEDGMTLHMVKNPDIVASVAALTNQRPFTVGFAAETQDVEKYARGKLERKNLDMIC
HHCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCEEE
ANDVSVQGQGFNSDNNALHLYWKEGDKALPLTTKSELGKAIMLEIVEKL
ECCCEEECCCCCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ASLAGKRILLGISGGIAAYKCAELTRRLIERGAEVRVVMTKAAKEFITPLTMQAVSGHP
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHCCCCC
VADSLLDPAAEASMGHIELAKWADIVLLAPATADLIARMAAGMGNDLLSTLVLATDSPVA
CHHHHHCCHHHCCCCCEEHHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
VSPAMNQQMYSNIATQENIATLARRGMHIWGPAAGQQACGDVGMGRMLEPMELVHLCEDF
ECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHH
FQANSCEQAEADEKPLKGQSLLITAGPTREAIDPVRYISNHSSGKMGFAIAEAAAQLGAN
HCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
VTLVSGPVNLATPENVKRIDIESAEQMHGAVMKNAQSHSIFIACAAVADFKLSQVATQKL
EEEEECCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHH
KKTADEDGMTLHMVKNPDIVASVAALTNQRPFTVGFAAETQDVEKYARGKLERKNLDMIC
HHCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCEEE
ANDVSVQGQGFNSDNNALHLYWKEGDKALPLTTKSELGKAIMLEIVEKL
ECCCEEECCCCCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA