Click here to switch to the map view.

The map label for this gene is virB4 [H]

Identifier: 58617562

GI number: 58617562

Start: 1343161

End: 1345533

Strand: Reverse

Name: virB4 [H]

Synonym: ERGA_CDS_08350

Alternate gene names: 58617562

Gene position: 1345533-1343161 (Counterclockwise)

Preceding gene: 58617563

Following gene: 58617561

Centisome position: 89.71

GC content: 29.62

Gene sequence:

>2373_bases
ATGTCTTTTATTAAAGAGATAATAGGTAATTCTGATGTAGATAATTATACTATGAAGGAACATGATAGTACCTATAATAA
GGGAAATTTTGTTCCTGCAGCTTGCCATTATGATGAAAATACTATATTAAATAAAAACGGGGAACTTTTACAAATAATCA
AAATTGAAGATTATATACTTAATACACAAGATACTGACTTAAGAACAATTGTACGCAATAGTATTGCGAGTAGTGTTACG
ACACCTGAAGTTTCTTTTTGGATATATACAATAAGGAAGCTACATAAGTTTGATCTTTCACGGGAGAGTATAGATGATGT
GTCTGATGTTATTCATGAGACACATATGAAATATGTAAGTCAGCAAGTTACGTATATTAATGAACTTTATATTGCTGTAG
TAACACATCATCTGCCTGAGAATATAAAAAGTCTTTTAGGAGCTTTGTCATTTCCGTATGTTAAAAATAAACATAAAGAA
TTCTTAAAAAACAAGGTGATTAGATTAAATAAAATTACTGGAAGTATTTTAGAAAATTTAAAAGGGTTTGAAGCAAGGAA
ATTAGGATTATTACCAAATAATGAAGGTAAAATAAGGTCAGAGCTGATAGAATTTTTTTTCTATCTGACTACAATGAGGT
ATAAGGAGTGTTTACTTGATGTTGCTGACATTGCTGGTGTGTGTGCATGTTACGATATTAGCATGGGGTTTAATACATTT
AAAATATCGGATCGTAATAGTCATAAGTTTGGTGCTATTTTAGCAATTAAGGATTATCATGAGTCTCCATTAGATTCAGT
AGATGGATGTTTACAGCAAAATTGTGAATTTATGATTGTAGAAATCATGAAATTTACGAAGGGTAAAAATGTAGTAAAGT
TCTTCCAAAAGCAAGCTCAACTTTTAGAGGTAAGTAATGATATTCATTTGAAGCAAGCAACTAATATAGATGAATTTGTA
TCAACTGATGTGAGCTCTTGCTTAAATTTCTGTGAACGCAAAATTAATTTTGTTATAGCATCTGATACATTACAGCAACT
TCAAATAAATATAAATAAATTCATCATTTCACTATCATCACTTGGAATCTTATGTGTAAGATCTGATTTGACAATGGAAG
ATGATTTTTGGGCGCATTTACCTGGAAATTTTTCTCATGTTTTAAATATTAGATATACCTTAACTAAATATGCTTGTGCA
TTTGCTTTGTTACATTATTTCCCTTCAGGAGCATTGCAAGGAAACAAATGGGGGCAAGCAATTACTATCTTTTTTTCAAA
TAAGGGAAGACCTTACTTTTTCAGTTTCCATGTAAAAGATAGAGGACATACTTTAATGGTTGGTAGTCCACAATCTTCTG
TTACTGTATTATTACATTTTTTATTATTAGAATCTAGAAAATTTGATACACGAATTATCATATTAGATTATAGGGGGAAG
TCTATTATTTTTACTAAAGCTATTAATGGGAAATATTATAGAGCAGATCATAGACCAGATCATCATGAAGTATCATTTAA
TTTTTTTCAACTTAAGGATAGTGATGTAAATCGTAAGATAATTAGTGGTATTTTACAAAGGATGTCATGTGTAAATGTAG
CAAATATGTCTGAAGAGATGAAAAATACTATTGATGAGATAGTAGAGTACATATTTAGTTTACCTATTGAGGATAGAAAT
GTAGGTAATATTACTGATCATGTTACTAGATTAGGTAAAAATGCAAGTAGGTGGGTAAATGATGGAGAATTTGCTCATCT
ATTAAAAGATGGTGTAAACATTGATTGGAATGCAAAGATTTTAGGTCTCAATGTTGGGGTGGTCTTATCTAATCCACAAT
GTGCTGCTGTTATAATATATTATTTTTTAAATATATTGGTGAATTATCTAGATGGATCTCCTACGATTTTAGTAGTAGAT
GAATCATGGATTTTAGATTACGTTTTTACCAGTGATCAAGAACTTGATGACTGGGTTAAAATGATGTCTCAATTAAATGT
TGTCATTATTTTTTCTGGTGAGAATATTCCAGCTGTTGTGTCAAGTAGTGTAATTTGTGGTTTTAATAAACATGTTGAAA
CACAAATTTTTATGCCAAATTCTGTATCAACTAATAAATTATACATGAAAGCTTTTAATCTTTCAAAAACAGAATGTAAT
ACTATGTTTCAGATGCCATCTCAAGAAGGGTATTTTTTTGTGAAACAATGTAATGTTTCAATAGTCTTATCATTTCAATT
ACCTAATATACCAGAAGCTAATGTGTTAACCGCTACTCAAGAAACGATACGATATATGTATGAATCTATTAATAGTTGTA
GTAATGATGTTAAGGAATGGTTACCTGCATTCTATAGCAAGTGTAGTAAGTAG

Upstream 100 bases:

>100_bases
TCTAGATAATGGTTAGGTGCGTATTGTTTGTATGTATATGTTGATCGATAATGAACACTTTTTTTTGATATTATTATAGG
TTTAAAAATGAGGTTTCTTC

Downstream 100 bases:

>100_bases
TGTTAATTCTAGATTTTTTTATATACTAATTGCAAATATTTAATATGTAATAGCAATAGCTTGGTTTTTAGACTATCAGT
TATATTAAGAGATTATATAT

Product: VirB4 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 790; Mature: 789

Protein sequence:

>790_residues
MSFIKEIIGNSDVDNYTMKEHDSTYNKGNFVPAACHYDENTILNKNGELLQIIKIEDYILNTQDTDLRTIVRNSIASSVT
TPEVSFWIYTIRKLHKFDLSRESIDDVSDVIHETHMKYVSQQVTYINELYIAVVTHHLPENIKSLLGALSFPYVKNKHKE
FLKNKVIRLNKITGSILENLKGFEARKLGLLPNNEGKIRSELIEFFFYLTTMRYKECLLDVADIAGVCACYDISMGFNTF
KISDRNSHKFGAILAIKDYHESPLDSVDGCLQQNCEFMIVEIMKFTKGKNVVKFFQKQAQLLEVSNDIHLKQATNIDEFV
STDVSSCLNFCERKINFVIASDTLQQLQININKFIISLSSLGILCVRSDLTMEDDFWAHLPGNFSHVLNIRYTLTKYACA
FALLHYFPSGALQGNKWGQAITIFFSNKGRPYFFSFHVKDRGHTLMVGSPQSSVTVLLHFLLLESRKFDTRIIILDYRGK
SIIFTKAINGKYYRADHRPDHHEVSFNFFQLKDSDVNRKIISGILQRMSCVNVANMSEEMKNTIDEIVEYIFSLPIEDRN
VGNITDHVTRLGKNASRWVNDGEFAHLLKDGVNIDWNAKILGLNVGVVLSNPQCAAVIIYYFLNILVNYLDGSPTILVVD
ESWILDYVFTSDQELDDWVKMMSQLNVVIIFSGENIPAVVSSSVICGFNKHVETQIFMPNSVSTNKLYMKAFNLSKTECN
TMFQMPSQEGYFFVKQCNVSIVLSFQLPNIPEANVLTATQETIRYMYESINSCSNDVKEWLPAFYSKCSK

Sequences:

>Translated_790_residues
MSFIKEIIGNSDVDNYTMKEHDSTYNKGNFVPAACHYDENTILNKNGELLQIIKIEDYILNTQDTDLRTIVRNSIASSVT
TPEVSFWIYTIRKLHKFDLSRESIDDVSDVIHETHMKYVSQQVTYINELYIAVVTHHLPENIKSLLGALSFPYVKNKHKE
FLKNKVIRLNKITGSILENLKGFEARKLGLLPNNEGKIRSELIEFFFYLTTMRYKECLLDVADIAGVCACYDISMGFNTF
KISDRNSHKFGAILAIKDYHESPLDSVDGCLQQNCEFMIVEIMKFTKGKNVVKFFQKQAQLLEVSNDIHLKQATNIDEFV
STDVSSCLNFCERKINFVIASDTLQQLQININKFIISLSSLGILCVRSDLTMEDDFWAHLPGNFSHVLNIRYTLTKYACA
FALLHYFPSGALQGNKWGQAITIFFSNKGRPYFFSFHVKDRGHTLMVGSPQSSVTVLLHFLLLESRKFDTRIIILDYRGK
SIIFTKAINGKYYRADHRPDHHEVSFNFFQLKDSDVNRKIISGILQRMSCVNVANMSEEMKNTIDEIVEYIFSLPIEDRN
VGNITDHVTRLGKNASRWVNDGEFAHLLKDGVNIDWNAKILGLNVGVVLSNPQCAAVIIYYFLNILVNYLDGSPTILVVD
ESWILDYVFTSDQELDDWVKMMSQLNVVIIFSGENIPAVVSSSVICGFNKHVETQIFMPNSVSTNKLYMKAFNLSKTECN
TMFQMPSQEGYFFVKQCNVSIVLSFQLPNIPEANVLTATQETIRYMYESINSCSNDVKEWLPAFYSKCSK
>Mature_789_residues
SFIKEIIGNSDVDNYTMKEHDSTYNKGNFVPAACHYDENTILNKNGELLQIIKIEDYILNTQDTDLRTIVRNSIASSVTT
PEVSFWIYTIRKLHKFDLSRESIDDVSDVIHETHMKYVSQQVTYINELYIAVVTHHLPENIKSLLGALSFPYVKNKHKEF
LKNKVIRLNKITGSILENLKGFEARKLGLLPNNEGKIRSELIEFFFYLTTMRYKECLLDVADIAGVCACYDISMGFNTFK
ISDRNSHKFGAILAIKDYHESPLDSVDGCLQQNCEFMIVEIMKFTKGKNVVKFFQKQAQLLEVSNDIHLKQATNIDEFVS
TDVSSCLNFCERKINFVIASDTLQQLQININKFIISLSSLGILCVRSDLTMEDDFWAHLPGNFSHVLNIRYTLTKYACAF
ALLHYFPSGALQGNKWGQAITIFFSNKGRPYFFSFHVKDRGHTLMVGSPQSSVTVLLHFLLLESRKFDTRIIILDYRGKS
IIFTKAINGKYYRADHRPDHHEVSFNFFQLKDSDVNRKIISGILQRMSCVNVANMSEEMKNTIDEIVEYIFSLPIEDRNV
GNITDHVTRLGKNASRWVNDGEFAHLLKDGVNIDWNAKILGLNVGVVLSNPQCAAVIIYYFLNILVNYLDGSPTILVVDE
SWILDYVFTSDQELDDWVKMMSQLNVVIIFSGENIPAVVSSSVICGFNKHVETQIFMPNSVSTNKLYMKAFNLSKTECNT
MFQMPSQEGYFFVKQCNVSIVLSFQLPNIPEANVLTATQETIRYMYESINSCSNDVKEWLPAFYSKCSK

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 90357; Mature: 90226

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIKEIIGNSDVDNYTMKEHDSTYNKGNFVPAACHYDENTILNKNGELLQIIKIEDYIL
CCHHHHHHCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCEECCCCCEEEEEEEEEEEE
NTQDTDLRTIVRNSIASSVTTPEVSFWIYTIRKLHKFDLSRESIDDVSDVIHETHMKYVS
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QQVTYINELYIAVVTHHLPENIKSLLGALSFPYVKNKHKEFLKNKVIRLNKITGSILENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEHHHHHHHHHC
KGFEARKLGLLPNNEGKIRSELIEFFFYLTTMRYKECLLDVADIAGVCACYDISMGFNTF
CCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEE
KISDRNSHKFGAILAIKDYHESPLDSVDGCLQQNCEFMIVEIMKFTKGKNVVKFFQKQAQ
EECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHHH
LLEVSNDIHLKQATNIDEFVSTDVSSCLNFCERKINFVIASDTLQQLQININKFIISLSS
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHC
LGILCVRSDLTMEDDFWAHLPGNFSHVLNIRYTLTKYACAFALLHYFPSGALQGNKWGQA
CCEEEEECCCCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
ITIFFSNKGRPYFFSFHVKDRGHTLMVGSPQSSVTVLLHFLLLESRKFDTRIIILDYRGK
EEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
SIIFTKAINGKYYRADHRPDHHEVSFNFFQLKDSDVNRKIISGILQRMSCVNVANMSEEM
EEEEEEECCCCEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNTIDEIVEYIFSLPIEDRNVGNITDHVTRLGKNASRWVNDGEFAHLLKDGVNIDWNAKI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCCCCCHHHHHHHCCCCCCCCCEE
LGLNVGVVLSNPQCAAVIIYYFLNILVNYLDGSPTILVVDESWILDYVFTSDQELDDWVK
EEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCCHHHHHH
MMSQLNVVIIFSGENIPAVVSSSVICGFNKHVETQIFMPNSVSTNKLYMKAFNLSKTECN
HHHCCCEEEEECCCCCCHHHCCCEEECCCCCCEEEEECCCCCCCCEEEEEEECCCHHHHH
TMFQMPSQEGYFFVKQCNVSIVLSFQLPNIPEANVLTATQETIRYMYESINSCSNDVKEW
HHHCCCCCCCEEEEEECCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LPAFYSKCSK
HHHHHHHHCC
>Mature Secondary Structure 
SFIKEIIGNSDVDNYTMKEHDSTYNKGNFVPAACHYDENTILNKNGELLQIIKIEDYIL
CHHHHHHCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCEECCCCCEEEEEEEEEEEE
NTQDTDLRTIVRNSIASSVTTPEVSFWIYTIRKLHKFDLSRESIDDVSDVIHETHMKYVS
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QQVTYINELYIAVVTHHLPENIKSLLGALSFPYVKNKHKEFLKNKVIRLNKITGSILENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEHHHHHHHHHC
KGFEARKLGLLPNNEGKIRSELIEFFFYLTTMRYKECLLDVADIAGVCACYDISMGFNTF
CCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEE
KISDRNSHKFGAILAIKDYHESPLDSVDGCLQQNCEFMIVEIMKFTKGKNVVKFFQKQAQ
EECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHHH
LLEVSNDIHLKQATNIDEFVSTDVSSCLNFCERKINFVIASDTLQQLQININKFIISLSS
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHC
LGILCVRSDLTMEDDFWAHLPGNFSHVLNIRYTLTKYACAFALLHYFPSGALQGNKWGQA
CCEEEEECCCCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
ITIFFSNKGRPYFFSFHVKDRGHTLMVGSPQSSVTVLLHFLLLESRKFDTRIIILDYRGK
EEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
SIIFTKAINGKYYRADHRPDHHEVSFNFFQLKDSDVNRKIISGILQRMSCVNVANMSEEM
EEEEEEECCCCEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNTIDEIVEYIFSLPIEDRNVGNITDHVTRLGKNASRWVNDGEFAHLLKDGVNIDWNAKI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCCCCCHHHHHHHCCCCCCCCCEE
LGLNVGVVLSNPQCAAVIIYYFLNILVNYLDGSPTILVVDESWILDYVFTSDQELDDWVK
EEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCCHHHHHH
MMSQLNVVIIFSGENIPAVVSSSVICGFNKHVETQIFMPNSVSTNKLYMKAFNLSKTECN
HHHCCCEEEEECCCCCCHHHCCCEEECCCCCCEEEEECCCCCCCCEEEEEEECCCHHHHH
TMFQMPSQEGYFFVKQCNVSIVLSFQLPNIPEANVLTATQETIRYMYESINSCSNDVKEW
HHHCCCCCCCEEEEEECCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LPAFYSKCSK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]