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The map label for this gene is metG [H]

Identifier: 58617532

GI number: 58617532

Start: 1288590

End: 1290110

Strand: Reverse

Name: metG [H]

Synonym: ERGA_CDS_08050

Alternate gene names: 58617532

Gene position: 1290110-1288590 (Counterclockwise)

Preceding gene: 58617537

Following gene: 58617527

Centisome position: 86.01

GC content: 31.03

Gene sequence:

>1521_bases
ATGAGAAATATATATGTGACTACTCCTATTTATTATGTTAATGATGTTCCACATATAGGGCATGTCTATACAACTTTAAT
ATCTGATATTATTGCAAGGTTTAAGCGTCTTGATGGTTATAATGTAAAGTTTATTACTGGGACTGATGAACATGGTCAGA
AAATAGAAAAAGCAGCTGCAAAGGCTAATAAATCATTAGTGGAGTTTACTGATCATGCTAGTGCTATTTTTCAACACTTA
ACAGGTGTTATGAACTTTAGTCATGATGATTTTATTCGTACAACGGAAATCAGGCATAAAAAGGCAGTTACAGAATTATG
GAGACGTTTATATCATTATGGGAAGATATATTTAGGTAATTATTCTGGATGGTATGCAGTTAGGGATGAAACATTTTACC
AAGAGAAAGAGTTAATTGATGGCAAAGCTCCTACTGGTGCTGAAGTAGAATGGGTAGAAGAGCCGAGTTATTTTTTTCGG
TTATCTCATTTTCAAGAAAGGTTATTAGATTTTTATGATAAACATCCGAATTTTATTATACCTAGACATCGTTATAATGA
AGTTATCTCTTTTGTTAAGTCTGGATTGAAAGATATTTCTATATCACGACAAAATATTTCTTGGGGGATAAGTGTACCGG
AGGATGATAATCATATTATTTATGTATGGATTGATGCTTTAACAAATTACTTAACAACTCTGGGGTTTCCTGAGATTAAT
AGTAGTGAATATAAAAATTATTGGGTTGATGAAAATAGTACAGTTGTTCATGTAGTAGGAAAGGATATATTGAGATTTCA
TGCAGTATATTGGCCAGCTATTTTAATGGCTGCTGAGTTACCATTGCCTAAACAAATTTTAGCACATGGTTGGTGGACTA
ATGAAGGACAGAAGATTTCCAAATCATTAGGAAATGTAATTAATCCTTTTGAATTAATTGAAGAATTTGGTGTTGATCAG
GTGAGGTACTTCTTAATTAAAGAAATGCCAGTAGGTAATGATGGTGATTATAAGAGAAATAGTATTATTAGTTGTATAAA
TTGTGATTTAGCAAATAATATTGGTAATTTAGTACAGAGAACTGTTTCGCTTTTGCATAAAGAATGTCATGGAACAATAC
CAAATGTGGCAAGTAGTCTATTTCAGGATGAGGATGCTTTACCAAATTATCAAGAGATTTTGGAAAATGTTAGAATTTGT
ATTAATCAGTACAACCTTAATGGTGTGATACATATTGTAGAAGAATTGTCGTCTATGGCAAATGAATATATTGCATTTAG
AGCTCCATGGAAGTTATCTAAAACTGATCCTATAGTTATGCAAGCTGTGTTATATAAGTTACTTGAATATATAAGATGTG
TTGGATTGCTATTGCAACCTATAATGCCATATTTATCATCTAAAATATTGGATCAGATTGCATTATCAGAGTACAAACGT
AGTTTTAATAATTTTGCTGTACCTATAGATACGGGAATAATTTTACCAAAACCTGAACCTATTTTTACAAAAATTATATA
A

Upstream 100 bases:

>100_bases
GCATACTGTATCTAGTATAGTATAAATGTGTATTGTATCCTCTAAATGGAATTATTGTGATATATTGATTGTTATTATTC
AGAAGTGATATTGTTGTTTT

Downstream 100 bases:

>100_bases
TTTTGATAATAGTGTAATAAAATATTGTCCTATATTATTCAATATTTCAAGTAATGTTAAACGTTATGTCAATGAATGTA
ATAGTATACTAATATCGACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MRNIYVTTPIYYVNDVPHIGHVYTTLISDIIARFKRLDGYNVKFITGTDEHGQKIEKAAAKANKSLVEFTDHASAIFQHL
TGVMNFSHDDFIRTTEIRHKKAVTELWRRLYHYGKIYLGNYSGWYAVRDETFYQEKELIDGKAPTGAEVEWVEEPSYFFR
LSHFQERLLDFYDKHPNFIIPRHRYNEVISFVKSGLKDISISRQNISWGISVPEDDNHIIYVWIDALTNYLTTLGFPEIN
SSEYKNYWVDENSTVVHVVGKDILRFHAVYWPAILMAAELPLPKQILAHGWWTNEGQKISKSLGNVINPFELIEEFGVDQ
VRYFLIKEMPVGNDGDYKRNSIISCINCDLANNIGNLVQRTVSLLHKECHGTIPNVASSLFQDEDALPNYQEILENVRIC
INQYNLNGVIHIVEELSSMANEYIAFRAPWKLSKTDPIVMQAVLYKLLEYIRCVGLLLQPIMPYLSSKILDQIALSEYKR
SFNNFAVPIDTGIILPKPEPIFTKII

Sequences:

>Translated_506_residues
MRNIYVTTPIYYVNDVPHIGHVYTTLISDIIARFKRLDGYNVKFITGTDEHGQKIEKAAAKANKSLVEFTDHASAIFQHL
TGVMNFSHDDFIRTTEIRHKKAVTELWRRLYHYGKIYLGNYSGWYAVRDETFYQEKELIDGKAPTGAEVEWVEEPSYFFR
LSHFQERLLDFYDKHPNFIIPRHRYNEVISFVKSGLKDISISRQNISWGISVPEDDNHIIYVWIDALTNYLTTLGFPEIN
SSEYKNYWVDENSTVVHVVGKDILRFHAVYWPAILMAAELPLPKQILAHGWWTNEGQKISKSLGNVINPFELIEEFGVDQ
VRYFLIKEMPVGNDGDYKRNSIISCINCDLANNIGNLVQRTVSLLHKECHGTIPNVASSLFQDEDALPNYQEILENVRIC
INQYNLNGVIHIVEELSSMANEYIAFRAPWKLSKTDPIVMQAVLYKLLEYIRCVGLLLQPIMPYLSSKILDQIALSEYKR
SFNNFAVPIDTGIILPKPEPIFTKII
>Mature_506_residues
MRNIYVTTPIYYVNDVPHIGHVYTTLISDIIARFKRLDGYNVKFITGTDEHGQKIEKAAAKANKSLVEFTDHASAIFQHL
TGVMNFSHDDFIRTTEIRHKKAVTELWRRLYHYGKIYLGNYSGWYAVRDETFYQEKELIDGKAPTGAEVEWVEEPSYFFR
LSHFQERLLDFYDKHPNFIIPRHRYNEVISFVKSGLKDISISRQNISWGISVPEDDNHIIYVWIDALTNYLTTLGFPEIN
SSEYKNYWVDENSTVVHVVGKDILRFHAVYWPAILMAAELPLPKQILAHGWWTNEGQKISKSLGNVINPFELIEEFGVDQ
VRYFLIKEMPVGNDGDYKRNSIISCINCDLANNIGNLVQRTVSLLHKECHGTIPNVASSLFQDEDALPNYQEILENVRIC
INQYNLNGVIHIVEELSSMANEYIAFRAPWKLSKTDPIVMQAVLYKLLEYIRCVGLLLQPIMPYLSSKILDQIALSEYKR
SFNNFAVPIDTGIILPKPEPIFTKII

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=514, Percent_Identity=36.9649805447471, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI14043022, Length=565, Percent_Identity=25.1327433628319, Blast_Score=142, Evalue=9e-34,
Organism=Escherichia coli, GI1788432, Length=504, Percent_Identity=24.0079365079365, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI212645096, Length=503, Percent_Identity=37.7733598409543, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI71992781, Length=390, Percent_Identity=36.9230769230769, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17540794, Length=557, Percent_Identity=24.4165170556553, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=34.7663551401869, Blast_Score=294, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=24.3243243243243, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21358483, Length=506, Percent_Identity=35.7707509881423, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24655311, Length=567, Percent_Identity=27.336860670194, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58469; Mature: 58469

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIYVTTPIYYVNDVPHIGHVYTTLISDIIARFKRLDGYNVKFITGTDEHGQKIEKAAA
CCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
KANKSLVEFTDHASAIFQHLTGVMNFSHDDFIRTTEIRHKKAVTELWRRLYHYGKIYLGN
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
YSGWYAVRDETFYQEKELIDGKAPTGAEVEWVEEPSYFFRLSHFQERLLDFYDKHPNFII
CCCEEEECCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEE
PRHRYNEVISFVKSGLKDISISRQNISWGISVPEDDNHIIYVWIDALTNYLTTLGFPEIN
CCHHHHHHHHHHHHCHHHCEEECCCCEECCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCC
SSEYKNYWVDENSTVVHVVGKDILRFHAVYWPAILMAAELPLPKQILAHGWWTNEGQKIS
CCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHH
KSLGNVINPFELIEEFGVDQVRYFLIKEMPVGNDGDYKRNSIISCINCDLANNIGNLVQR
HHHHHCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHCHHHHCCCHHHHHHHHHHHH
TVSLLHKECHGTIPNVASSLFQDEDALPNYQEILENVRICINQYNLNGVIHIVEELSSMA
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
NEYIAFRAPWKLSKTDPIVMQAVLYKLLEYIRCVGLLLQPIMPYLSSKILDQIALSEYKR
HHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFNNFAVPIDTGIILPKPEPIFTKII
HHCCEEEEECCCEECCCCCCHHHCCC
>Mature Secondary Structure
MRNIYVTTPIYYVNDVPHIGHVYTTLISDIIARFKRLDGYNVKFITGTDEHGQKIEKAAA
CCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
KANKSLVEFTDHASAIFQHLTGVMNFSHDDFIRTTEIRHKKAVTELWRRLYHYGKIYLGN
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
YSGWYAVRDETFYQEKELIDGKAPTGAEVEWVEEPSYFFRLSHFQERLLDFYDKHPNFII
CCCEEEECCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEE
PRHRYNEVISFVKSGLKDISISRQNISWGISVPEDDNHIIYVWIDALTNYLTTLGFPEIN
CCHHHHHHHHHHHHCHHHCEEECCCCEECCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCC
SSEYKNYWVDENSTVVHVVGKDILRFHAVYWPAILMAAELPLPKQILAHGWWTNEGQKIS
CCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHH
KSLGNVINPFELIEEFGVDQVRYFLIKEMPVGNDGDYKRNSIISCINCDLANNIGNLVQR
HHHHHCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHCHHHHCCCHHHHHHHHHHHH
TVSLLHKECHGTIPNVASSLFQDEDALPNYQEILENVRICINQYNLNGVIHIVEELSSMA
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
NEYIAFRAPWKLSKTDPIVMQAVLYKLLEYIRCVGLLLQPIMPYLSSKILDQIALSEYKR
HHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFNNFAVPIDTGIILPKPEPIFTKII
HHCCEEEEECCCEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA