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The map label for this gene is bcr [H]

Identifier: 58617432

GI number: 58617432

Start: 1118597

End: 1119793

Strand: Reverse

Name: bcr [H]

Synonym: ERGA_CDS_07050

Alternate gene names: 58617432

Gene position: 1119793-1118597 (Counterclockwise)

Preceding gene: 58617433

Following gene: 58617426

Centisome position: 74.66

GC content: 28.74

Gene sequence:

>1197_bases
ATGAAAGTTTTTAAAAGGAATATTTTAATTCTTATTATTACAGTGATAGTAATGACTGAAATGTCATCAGATATATATTT
GCCTAGTTTGCCAAAAATTAGTGATTTTTTTCATGTAGATTATTCAATTGCACAGTTGACAATTAGTTTAAATTTAGCTG
GAATTTCTATTTCAGGTTTATTGTATGGTCCTCTATCTGATTATTATGGGCGTCGTCCTGTGATGTTGTTAGGTATTGTG
ATATTTACTATAGCAAGTGTAGCATGTTATTTTGCTAACAATGTTATGATGTTAATTATTATGCGTTTTATTCAAGGATT
TGGAACAGGAGTTGCAGGAGTTGTTGGATACGCTATTATCAAAGATATGTATTCAGGTGATGAATGTGCTAAAAATATTT
CTATAGTAAATATAGCAGTGGCATTTTCTCCAGTGTTAGGACCAATTTTAGGAAGTACAGTAATATCTTATGAGTATAAT
TGGAATGTACTGTTTATGATAATATCTGTTATATCTGTTATAGTTTTGATATGCTTATTTTCTTTTTTAAGAGAAACGTT
AGTTATTGATAGATCTAAAAATAATATTTCTTTTAGATCAGTTATGTATAAATATAAAGAACTTATCTGTAACTATAGAT
TCTTTGGATTCGCTTTGATTCATAGTTTGACTATTATGTGGATTTGGTCATGCATCACTAACTTACCATTTGTTTTTGTA
AATGATATGGGTGTACCTGTTGCTTACTATGGTTATTTTGTAGCTATTAATGTTGCTGCCTATATTATAGGCGCTATTAT
TAACCAGAAATTTGTTGAGAAGTTTGGTATGAGTAATATGCTGATTTTGGGTTTAATTTTAACAACCTTGTCAGATGCGA
GTGTTTTTATTTTGTATCAGGTGATAGAAATAACTCCTTTATTAGCAGAAATTATGTGGATCCCATCTGGTATGGGTATT
GCGTTTATTCTTGGAAATAATATGACTTGTGCTTTTTCTGAGATTAAAGAAACAGGTATAGGTTCAGCATTTATTTTATT
TTTACAAACGATTTTTGGTGCTATTGGCATTTATATTTTAAGTTGTTTTTATGATGGTACTTTGATACCTGTTATAATTT
TTCCAGTAGTTTGTTCTATATTATGTATTATCATATACTTTGTTCTTAAGGTAACGGAAAAAAAACCTAATATGTAG

Upstream 100 bases:

>100_bases
ATTTTTTATATTACATTAATAATGAAATAAAGCTTAAGTATTATAGCTTTGGCTTATATTAGATTATTTTATTAATTAAG
GGGGGTTTATTTTTTTATTT

Downstream 100 bases:

>100_bases
GAATGTAATTCTATTTTGATGATATCATAATAAAGTGTAACTTCTAAATGATAATTATATGGGTGGTTATAGTTAGTATA
CTTTATTAATTTGTGAGTAT

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKVFKRNILILIITVIVMTEMSSDIYLPSLPKISDFFHVDYSIAQLTISLNLAGISISGLLYGPLSDYYGRRPVMLLGIV
IFTIASVACYFANNVMMLIIMRFIQGFGTGVAGVVGYAIIKDMYSGDECAKNISIVNIAVAFSPVLGPILGSTVISYEYN
WNVLFMIISVISVIVLICLFSFLRETLVIDRSKNNISFRSVMYKYKELICNYRFFGFALIHSLTIMWIWSCITNLPFVFV
NDMGVPVAYYGYFVAINVAAYIIGAIINQKFVEKFGMSNMLILGLILTTLSDASVFILYQVIEITPLLAEIMWIPSGMGI
AFILGNNMTCAFSEIKETGIGSAFILFLQTIFGAIGIYILSCFYDGTLIPVIIFPVVCSILCIIIYFVLKVTEKKPNM

Sequences:

>Translated_398_residues
MKVFKRNILILIITVIVMTEMSSDIYLPSLPKISDFFHVDYSIAQLTISLNLAGISISGLLYGPLSDYYGRRPVMLLGIV
IFTIASVACYFANNVMMLIIMRFIQGFGTGVAGVVGYAIIKDMYSGDECAKNISIVNIAVAFSPVLGPILGSTVISYEYN
WNVLFMIISVISVIVLICLFSFLRETLVIDRSKNNISFRSVMYKYKELICNYRFFGFALIHSLTIMWIWSCITNLPFVFV
NDMGVPVAYYGYFVAINVAAYIIGAIINQKFVEKFGMSNMLILGLILTTLSDASVFILYQVIEITPLLAEIMWIPSGMGI
AFILGNNMTCAFSEIKETGIGSAFILFLQTIFGAIGIYILSCFYDGTLIPVIIFPVVCSILCIIIYFVLKVTEKKPNM
>Mature_398_residues
MKVFKRNILILIITVIVMTEMSSDIYLPSLPKISDFFHVDYSIAQLTISLNLAGISISGLLYGPLSDYYGRRPVMLLGIV
IFTIASVACYFANNVMMLIIMRFIQGFGTGVAGVVGYAIIKDMYSGDECAKNISIVNIAVAFSPVLGPILGSTVISYEYN
WNVLFMIISVISVIVLICLFSFLRETLVIDRSKNNISFRSVMYKYKELICNYRFFGFALIHSLTIMWIWSCITNLPFVFV
NDMGVPVAYYGYFVAINVAAYIIGAIINQKFVEKFGMSNMLILGLILTTLSDASVFILYQVIEITPLLAEIMWIPSGMGI
AFILGNNMTCAFSEIKETGIGSAFILFLQTIFGAIGIYILSCFYDGTLIPVIIFPVVCSILCIIIYFVLKVTEKKPNM

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=269, Percent_Identity=30.8550185873606, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994889, Length=298, Percent_Identity=27.8523489932886, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790794, Length=255, Percent_Identity=29.8039215686275, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790146, Length=240, Percent_Identity=28.3333333333333, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787065, Length=266, Percent_Identity=25.187969924812, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082312, Length=195, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789207, Length=149, Percent_Identity=28.8590604026846, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081983, Length=155, Percent_Identity=28.3870967741935, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=206, Percent_Identity=28.1553398058252, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321838, Length=208, Percent_Identity=26.4423076923077, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323000, Length=178, Percent_Identity=28.6516853932584, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324264, Length=168, Percent_Identity=25.5952380952381, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=172, Percent_Identity=26.7441860465116, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44356; Mature: 44356

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFKRNILILIITVIVMTEMSSDIYLPSLPKISDFFHVDYSIAQLTISLNLAGISISGL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEEEEEEEEEEEEEEEEEEE
LYGPLSDYYGRRPVMLLGIVIFTIASVACYFANNVMMLIIMRFIQGFGTGVAGVVGYAII
EECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KDMYSGDECAKNISIVNIAVAFSPVLGPILGSTVISYEYNWNVLFMIISVISVIVLICLF
HHHHCCHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
SFLRETLVIDRSKNNISFRSVMYKYKELICNYRFFGFALIHSLTIMWIWSCITNLPFVFV
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
NDMGVPVAYYGYFVAINVAAYIIGAIINQKFVEKFGMSNMLILGLILTTLSDASVFILYQ
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
VIEITPLLAEIMWIPSGMGIAFILGNNMTCAFSEIKETGIGSAFILFLQTIFGAIGIYIL
HHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SCFYDGTLIPVIIFPVVCSILCIIIYFVLKVTEKKPNM
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKVFKRNILILIITVIVMTEMSSDIYLPSLPKISDFFHVDYSIAQLTISLNLAGISISGL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEEEEEEEEEEEEEEEEEEE
LYGPLSDYYGRRPVMLLGIVIFTIASVACYFANNVMMLIIMRFIQGFGTGVAGVVGYAII
EECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KDMYSGDECAKNISIVNIAVAFSPVLGPILGSTVISYEYNWNVLFMIISVISVIVLICLF
HHHHCCHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
SFLRETLVIDRSKNNISFRSVMYKYKELICNYRFFGFALIHSLTIMWIWSCITNLPFVFV
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
NDMGVPVAYYGYFVAINVAAYIIGAIINQKFVEKFGMSNMLILGLILTTLSDASVFILYQ
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
VIEITPLLAEIMWIPSGMGIAFILGNNMTCAFSEIKETGIGSAFILFLQTIFGAIGIYIL
HHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SCFYDGTLIPVIIFPVVCSILCIIIYFVLKVTEKKPNM
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]