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The map label for this gene is ntrX [C]

Identifier: 58617411

GI number: 58617411

Start: 1085237

End: 1086652

Strand: Reverse

Name: ntrX [C]

Synonym: ERGA_CDS_06840

Alternate gene names: 58617411

Gene position: 1086652-1085237 (Counterclockwise)

Preceding gene: 58617412

Following gene: 58617409

Centisome position: 72.45

GC content: 32.42

Gene sequence:

>1416_bases
ATGGCACAGGATTTTGAAATGTCCAAGGAAAGATTGTATATTTCTGAAGTATTAGTTGTTGATGATGAAGTTGATATCAG
AAATCTAATAAAAGATATATTAAGTGATGATAATTATGTCACTAAATTAGCAGTTGATGGTTTATCCGCGATCAAGATGG
CTTATGAAAAAGAGCCTGATGTTGTATTATTGGATATATGGTTAAGAGGATCTGATATTGATGGATTAAGTGTACTGGAG
AAGCTTAAAGAAAGGTATCCTTATTTGCCTGTTATTATGATTAGTGGGCATGGTAATATTGCCACTGCTGTAAAGTCTCT
GCATATGGGTGCTTATGATTATATAGAAAAGCCTTTTACAGAAGGAAGATTAAAGTTAGTTGTAAAGAGAGCTATAGAGT
CTGGTAGATTACGTAGAGAAAATGATGAGTTGAAATCAGCATTTGAGGATTATGAAATAGTCGGTAACTCCCCTGTTATA
CGTAATTTGAGAAGTATGATTAATAAAGCAGCTACTACATCGAGTCGTATACTCATTACTGGTTCGCCAGGTGTTGGAAA
GGAAGTAGTTGCTAGGCTAATACATAAAAAATCCAAGGGGTATGATACTCCATTTATATCTATGTACTCATCTATGCTAC
CAGCTAATAATTACTTGGTTAATATATTTGGTAGTGAGGAAAGTAATAATATATTGTCTCATAGAGTACCTCCTCATATT
GGAATTATAGAGCAAGCAAATCATGGTACGTTATTTATAGATGAAGTAACAGATTTACGATACGATACGCAATTAAGATT
ACTCAGATTATTACAGGAGGGAAAAATATATAGGGAAAATAGTAAGATTCCTGTTAGTATAGATGTGAGAATTATTGTGT
CTTCTTCCAAAGATATTGAAAGTGAAGTAAAAGCTGGTAGGTTTTGTGAGGATTTATATTATAGATTAAATGTCCTTCCA
ATTAGAGTACCGTCTTTAGTAGAATATTGTACAGATATACCGGAATTGTGTAGGTATTTTATGAATAGCATCTGTAAAAA
AATAGGTTTGTGTACTCATGTATTAAGTGATGAAGCTTTAATAGCAATGCAGTCATATGAATGGCCAGGTAACTTAAGAC
AATTACGTAATGTTATAGAATGGATTTTAATTATGAAATCTCCTAAGGAGATGATTACAGCAAAAGATTTACCAGTAGAT
ATAGTATCTAATTCGCCTATTAATGATGTTTTAAGTGCTAAAGTTATTTCTGTACCATTACGTAAAGCTCGTGAAGAATT
TGAAAGACAGTATTTAAAAACTCAGTTATCTCGTTTTGGAGGTAATGTATCACGAACTGCTGAATTTGTTGGAATGGAAC
GTTCAGCATTACACCGTAAATTGAAAATTCTTGGATTGTGTAATGTTTCTGAATAA

Upstream 100 bases:

>100_bases
TATATTGGTTGTTGATCATTAGTGTGTGTGGTAGTATTATGAGGTGTACTTTAAAGGAGTACATTGTGTTTGCAGTATTT
CATTAGTATTGTAGGTAATT

Downstream 100 bases:

>100_bases
ACATTGCATGTATAATTATTTATGTATGAATTATGAAGTCATTTTTTTTAAAAGGATATGATATATTATAGAATTTGATA
ACTATGTGTTGGTGAAGTAT

Product: nitrogen assimilation regulatory protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAQDFEMSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDIWLRGSDIDGLSVLE
KLKERYPYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKLVVKRAIESGRLRRENDELKSAFEDYEIVGNSPVI
RNLRSMINKAATTSSRILITGSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESNNILSHRVPPHI
GIIEQANHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSIDVRIIVSSSKDIESEVKAGRFCEDLYYRLNVLP
IRVPSLVEYCTDIPELCRYFMNSICKKIGLCTHVLSDEALIAMQSYEWPGNLRQLRNVIEWILIMKSPKEMITAKDLPVD
IVSNSPINDVLSAKVISVPLRKAREEFERQYLKTQLSRFGGNVSRTAEFVGMERSALHRKLKILGLCNVSE

Sequences:

>Translated_471_residues
MAQDFEMSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDIWLRGSDIDGLSVLE
KLKERYPYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKLVVKRAIESGRLRRENDELKSAFEDYEIVGNSPVI
RNLRSMINKAATTSSRILITGSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESNNILSHRVPPHI
GIIEQANHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSIDVRIIVSSSKDIESEVKAGRFCEDLYYRLNVLP
IRVPSLVEYCTDIPELCRYFMNSICKKIGLCTHVLSDEALIAMQSYEWPGNLRQLRNVIEWILIMKSPKEMITAKDLPVD
IVSNSPINDVLSAKVISVPLRKAREEFERQYLKTQLSRFGGNVSRTAEFVGMERSALHRKLKILGLCNVSE
>Mature_470_residues
AQDFEMSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPDVVLLDIWLRGSDIDGLSVLEK
LKERYPYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFTEGRLKLVVKRAIESGRLRRENDELKSAFEDYEIVGNSPVIR
NLRSMINKAATTSSRILITGSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESNNILSHRVPPHIG
IIEQANHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSIDVRIIVSSSKDIESEVKAGRFCEDLYYRLNVLPI
RVPSLVEYCTDIPELCRYFMNSICKKIGLCTHVLSDEALIAMQSYEWPGNLRQLRNVIEWILIMKSPKEMITAKDLPVDI
VSNSPINDVLSAKVISVPLRKAREEFERQYLKTQLSRFGGNVSRTAEFVGMERSALHRKLKILGLCNVSE

Specific function: Member of the two-component regulatory system RBE_0312/RBE_0470 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=32.6923076923077, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=31.9565217391304, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790437, Length=452, Percent_Identity=31.6371681415929, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788905, Length=448, Percent_Identity=29.0178571428571, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789233, Length=331, Percent_Identity=31.1178247734139, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=32.3076923076923, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=30.1169590643275, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI1789087, Length=318, Percent_Identity=32.7044025157233, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786524, Length=333, Percent_Identity=28.5285285285285, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=31.0769230769231, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081872, Length=307, Percent_Identity=27.6872964169381, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=28.2958199356913, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789828, Length=307, Percent_Identity=25.4071661237785, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787487, Length=145, Percent_Identity=28.2758620689655, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=139, Percent_Identity=30.2158273381295, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=34.1666666666667, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53492; Mature: 53361

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDFEMSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPD
CCCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHCCCCC
VVLLDIWLRGSDIDGLSVLEKLKERYPYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFT
EEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHCCCC
EGRLKLVVKRAIESGRLRRENDELKSAFEDYEIVGNSPVIRNLRSMINKAATTSSRILIT
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
GSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESNNILSHRVPPHI
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCCCCCC
GIIEQANHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSIDVRIIVSSSKDIE
CEEEECCCCEEEEECHHCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEEEEECCCCHH
SEVKAGRFCEDLYYRLNVLPIRVPSLVEYCTDIPELCRYFMNSICKKIGLCTHVLSDEAL
HHHHHHHHHHHHHHHHHEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IAMQSYEWPGNLRQLRNVIEWILIMKSPKEMITAKDLPVDIVSNSPINDVLSAKVISVPL
EEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
RKAREEFERQYLKTQLSRFGGNVSRTAEFVGMERSALHRKLKILGLCNVSE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHEEECCCCC
>Mature Secondary Structure 
AQDFEMSKERLYISEVLVVDDEVDIRNLIKDILSDDNYVTKLAVDGLSAIKMAYEKEPD
CCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHCCCCC
VVLLDIWLRGSDIDGLSVLEKLKERYPYLPVIMISGHGNIATAVKSLHMGAYDYIEKPFT
EEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHCCCC
EGRLKLVVKRAIESGRLRRENDELKSAFEDYEIVGNSPVIRNLRSMINKAATTSSRILIT
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
GSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESNNILSHRVPPHI
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCCCCCC
GIIEQANHGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKIPVSIDVRIIVSSSKDIE
CEEEECCCCEEEEECHHCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEEEEECCCCHH
SEVKAGRFCEDLYYRLNVLPIRVPSLVEYCTDIPELCRYFMNSICKKIGLCTHVLSDEAL
HHHHHHHHHHHHHHHHHEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IAMQSYEWPGNLRQLRNVIEWILIMKSPKEMITAKDLPVDIVSNSPINDVLSAKVISVPL
EEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
RKAREEFERQYLKTQLSRFGGNVSRTAEFVGMERSALHRKLKILGLCNVSE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA