Click here to switch to the map view.

The map label for this gene is ksgA

Identifier: 58617138

GI number: 58617138

Start: 681713

End: 682501

Strand: Reverse

Name: ksgA

Synonym: ERGA_CDS_04110

Alternate gene names: 58617138

Gene position: 682501-681713 (Counterclockwise)

Preceding gene: 58617143

Following gene: 58617131

Centisome position: 45.5

GC content: 25.98

Gene sequence:

>789_bases
ATGAATGATAATTACATGATAAATCCTAAAAAAGAGTTAAGCCAATGTTTCATTCACTCAACTGATATTACAGATAAAAT
AGTTAATTATGCAGGAAACATTAGCAATTTTTCAATTATTGAAATTGGTCCCGGACTTGGTACAATGACTTATTCAATAC
TCAAAAAGAATCCTAAAAAATTAATCTCTATAGAGAAAGACAGTAGATTATTACCAATACATGATAAAATAATTAAAGAA
TTTCAAGGTAAATATGAATTCATATTATCAGATGCATTAGATATTGATTTACGAAATATAGCGAAACCACCAGTAAAAGT
AATAGCAAATTTACCTTATAGTATTGCAACTCTACTTTTAATAAAGTGGATCAATTATATTAATTTCTTTCATAGCTTTA
CATTAATGTTCCAAAAAGAAGTAGCAGATCGTATAATAGCACAACCAAATAACAAAAATTATGGTACTTTATCTATACTA
ACTCAATTATTTGCTGATGTGTATAAAATGCAGGATTTTGGCCCTGAAATTTTCTCACCAAAACCTAAAGTGTTTTCATC
TGTAATAAATATTGTAGTACTACCACAACCTAGATTTGATGTAAATTATGATAAATTACGTAAAATTGTAAAAATAACTT
TTAACCAACGTCGTAAGATGATAAGAAGCACATTAAAGCAAATTACTAACAATACAGATGAAATTTTACATTCTTTAAAT
ATTCCAAATAATTTACGTCCTGAAAATTTATCAATAAAACAGTTCTGTGATATTGCAAATTGTATATAA

Upstream 100 bases:

>100_bases
CAATACTCTGATAAATTATAATTTGTATATATAAGCATACTACTTACCAGTATAATGATATTAGAAATTTACAAAATTTA
ATAATCCAATTTAAAAAATT

Downstream 100 bases:

>100_bases
TCTAAATATAAAAAAATCAATTATTAGTATTCATATAATACTAATATTTGGATAACTAATATTACGAGTATATAGCACTA
TACTTGTAATAGGTTAACTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNDNYMINPKKELSQCFIHSTDITDKIVNYAGNISNFSIIEIGPGLGTMTYSILKKNPKKLISIEKDSRLLPIHDKIIKE
FQGKYEFILSDALDIDLRNIAKPPVKVIANLPYSIATLLLIKWINYINFFHSFTLMFQKEVADRIIAQPNNKNYGTLSIL
TQLFADVYKMQDFGPEIFSPKPKVFSSVINIVVLPQPRFDVNYDKLRKIVKITFNQRRKMIRSTLKQITNNTDEILHSLN
IPNNLRPENLSIKQFCDIANCI

Sequences:

>Translated_262_residues
MNDNYMINPKKELSQCFIHSTDITDKIVNYAGNISNFSIIEIGPGLGTMTYSILKKNPKKLISIEKDSRLLPIHDKIIKE
FQGKYEFILSDALDIDLRNIAKPPVKVIANLPYSIATLLLIKWINYINFFHSFTLMFQKEVADRIIAQPNNKNYGTLSIL
TQLFADVYKMQDFGPEIFSPKPKVFSSVINIVVLPQPRFDVNYDKLRKIVKITFNQRRKMIRSTLKQITNNTDEILHSLN
IPNNLRPENLSIKQFCDIANCI
>Mature_262_residues
MNDNYMINPKKELSQCFIHSTDITDKIVNYAGNISNFSIIEIGPGLGTMTYSILKKNPKKLISIEKDSRLLPIHDKIIKE
FQGKYEFILSDALDIDLRNIAKPPVKVIANLPYSIATLLLIKWINYINFFHSFTLMFQKEVADRIIAQPNNKNYGTLSIL
TQLFADVYKMQDFGPEIFSPKPKVFSSVINIVVLPQPRFDVNYDKLRKIVKITFNQRRKMIRSTLKQITNNTDEILHSLN
IPNNLRPENLSIKQFCDIANCI

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=280, Percent_Identity=32.5, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI7657198, Length=241, Percent_Identity=32.7800829875519, Blast_Score=104, Evalue=8e-23,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25141369, Length=286, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=217, Percent_Identity=28.110599078341, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=236, Percent_Identity=29.2372881355932, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21357273, Length=279, Percent_Identity=28.3154121863799, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21358017, Length=219, Percent_Identity=32.4200913242009, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_EHRRG (Q5FH30)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196337.1
- ProteinModelPortal:   Q5FH30
- SMR:   Q5FH30
- STRING:   Q5FH30
- GeneID:   3268619
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_04110
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   ERUM302409:ERGA_CDS_04110-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30227; Mature: 30227

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 43-43 BINDING 65-65 BINDING 91-91 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNYMINPKKELSQCFIHSTDITDKIVNYAGNISNFSIIEIGPGLGTMTYSILKKNPKK
CCCCEECCCHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCHHH
LISIEKDSRLLPIHDKIIKEFQGKYEFILSDALDIDLRNIAKPPVKVIANLPYSIATLLL
HEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHHH
IKWINYINFFHSFTLMFQKEVADRIIAQPNNKNYGTLSILTQLFADVYKMQDFGPEIFSP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCC
KPKVFSSVINIVVLPQPRFDVNYDKLRKIVKITFNQRRKMIRSTLKQITNNTDEILHSLN
CHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
IPNNLRPENLSIKQFCDIANCI
CCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNDNYMINPKKELSQCFIHSTDITDKIVNYAGNISNFSIIEIGPGLGTMTYSILKKNPKK
CCCCEECCCHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCHHH
LISIEKDSRLLPIHDKIIKEFQGKYEFILSDALDIDLRNIAKPPVKVIANLPYSIATLLL
HEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHHH
IKWINYINFFHSFTLMFQKEVADRIIAQPNNKNYGTLSILTQLFADVYKMQDFGPEIFSP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCC
KPKVFSSVINIVVLPQPRFDVNYDKLRKIVKITFNQRRKMIRSTLKQITNNTDEILHSLN
CHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
IPNNLRPENLSIKQFCDIANCI
CCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA