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The map label for this gene is rpoH [H]

Identifier: 58617131

GI number: 58617131

Start: 666015

End: 666974

Strand: Reverse

Name: rpoH [H]

Synonym: ERGA_CDS_04040

Alternate gene names: 58617131

Gene position: 666974-666015 (Counterclockwise)

Preceding gene: 58617138

Following gene: 58617129

Centisome position: 44.47

GC content: 27.71

Gene sequence:

>960_bases
TTGAAAATTTACTATACATCATTTATATTAGTGGTAATAATTATAGTGCTATACAGGGTTATATTTTTCATTATGTTAAC
AAGTTCTATATTATCTCTAACTCAAGACAACTTAATGTCTTATATTAACGAAGTACAATCGTTTCAGGTTCTGTCCTTAG
AAGAAGAAGAAAAGTTAGCAAAAAATTGGTATGAGCACGGTGTTATTGCTGATGCCCATAAATTAGTTACTAGCCATTTA
AGATTAGTAGTAAAAGTTGCCTTAAGTTTTAAAAATTATGGGCTACCACTTATAGAACTAATCATGGAAGGAAATATAGG
GTTAATGCAAGCTGTTAAAAAGTTTAACCCTAATTTAGGTTTTAGACTCTCTACATATGCAATATGGTGGATTAAAGCTT
CAATAAAAGATTATATATTAAAGTCATGGTCATGCATAAAAATTGGAACAACACAAGCACAAAGAAAGTTATTCTTTAGT
TTAAGAAAAATTAAAAAAAAGCTCCTTAAGTACCATAACAATATCACAAAAGAAGATATTAAAATGATAGCAAATGAATG
TTCAACCTCTGAACAAGAAGTTGAACAAATGAACAAGTATTTTACCTATAGAGATAAATCACTAAATGATGTAGTATTCT
CTAATGGAAGCCAGAATGGTGTACAGCTACAAGATATGATAAAATGCGAAGATCCTAATCAAGAAGATGCATACTTATTA
AATGAAGAAACGGATATTAAGAAATCTTTAATTACGCAAGCATTAGCAACTTTAAACGAAAGACACCGTGATATATTTAT
TAGAAGAAAATTAATAGAAGAACCAGATACTCTAGAAATACTAAGTAAAAAGTATAATATCTCAAAAGAACGTATTAGAC
AAATAGAAACACATGCTTTCTTAAAAGTAAAAAACTTTGTTATATCTAAAAGAAAAGAATTTGGATTTCATAATATATAA

Upstream 100 bases:

>100_bases
AGATCTAATATTATCAGTAATATCAAAACCTCTTGTTGTAATAACTATATTCATAATGAGTATCTTAAAAAATTATACAT
TAAATTAATATATCAAAACC

Downstream 100 bases:

>100_bases
AAAAATTCATTTTTATCTCACTTATTGTATCAAGACTATATTATTAGGTACTTTTATTTGGATTTCTTGTACTATATTAT
CATAATTTAATAATACATTA

Product: RNA polymerase sigma-32 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKIYYTSFILVVIIIVLYRVIFFIMLTSSILSLTQDNLMSYINEVQSFQVLSLEEEEKLAKNWYEHGVIADAHKLVTSHL
RLVVKVALSFKNYGLPLIELIMEGNIGLMQAVKKFNPNLGFRLSTYAIWWIKASIKDYILKSWSCIKIGTTQAQRKLFFS
LRKIKKKLLKYHNNITKEDIKMIANECSTSEQEVEQMNKYFTYRDKSLNDVVFSNGSQNGVQLQDMIKCEDPNQEDAYLL
NEETDIKKSLITQALATLNERHRDIFIRRKLIEEPDTLEILSKKYNISKERIRQIETHAFLKVKNFVISKRKEFGFHNI

Sequences:

>Translated_319_residues
MKIYYTSFILVVIIIVLYRVIFFIMLTSSILSLTQDNLMSYINEVQSFQVLSLEEEEKLAKNWYEHGVIADAHKLVTSHL
RLVVKVALSFKNYGLPLIELIMEGNIGLMQAVKKFNPNLGFRLSTYAIWWIKASIKDYILKSWSCIKIGTTQAQRKLFFS
LRKIKKKLLKYHNNITKEDIKMIANECSTSEQEVEQMNKYFTYRDKSLNDVVFSNGSQNGVQLQDMIKCEDPNQEDAYLL
NEETDIKKSLITQALATLNERHRDIFIRRKLIEEPDTLEILSKKYNISKERIRQIETHAFLKVKNFVISKRKEFGFHNI
>Mature_319_residues
MKIYYTSFILVVIIIVLYRVIFFIMLTSSILSLTQDNLMSYINEVQSFQVLSLEEEEKLAKNWYEHGVIADAHKLVTSHL
RLVVKVALSFKNYGLPLIELIMEGNIGLMQAVKKFNPNLGFRLSTYAIWWIKASIKDYILKSWSCIKIGTTQAQRKLFFS
LRKIKKKLLKYHNNITKEDIKMIANECSTSEQEVEQMNKYFTYRDKSLNDVVFSNGSQNGVQLQDMIKCEDPNQEDAYLL
NEETDIKKSLITQALATLNERHRDIFIRRKLIEEPDTLEILSKKYNISKERIRQIETHAFLKVKNFVISKRKEFGFHNI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=270, Percent_Identity=35.9259259259259, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789098, Length=284, Percent_Identity=30.2816901408451, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789448, Length=249, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37425; Mature: 37425

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYYTSFILVVIIIVLYRVIFFIMLTSSILSLTQDNLMSYINEVQSFQVLSLEEEEKLA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KNWYEHGVIADAHKLVTSHLRLVVKVALSFKNYGLPLIELIMEGNIGLMQAVKKFNPNLG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCC
FRLSTYAIWWIKASIKDYILKSWSCIKIGTTQAQRKLFFSLRKIKKKLLKYHNNITKEDI
EEEEHHEEEEHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KMIANECSTSEQEVEQMNKYFTYRDKSLNDVVFSNGSQNGVQLQDMIKCEDPNQEDAYLL
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCEEE
NEETDIKKSLITQALATLNERHRDIFIRRKLIEEPDTLEILSKKYNISKERIRQIETHAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHH
LKVKNFVISKRKEFGFHNI
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKIYYTSFILVVIIIVLYRVIFFIMLTSSILSLTQDNLMSYINEVQSFQVLSLEEEEKLA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KNWYEHGVIADAHKLVTSHLRLVVKVALSFKNYGLPLIELIMEGNIGLMQAVKKFNPNLG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCC
FRLSTYAIWWIKASIKDYILKSWSCIKIGTTQAQRKLFFSLRKIKKKLLKYHNNITKEDI
EEEEHHEEEEHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KMIANECSTSEQEVEQMNKYFTYRDKSLNDVVFSNGSQNGVQLQDMIKCEDPNQEDAYLL
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCEEE
NEETDIKKSLITQALATLNERHRDIFIRRKLIEEPDTLEILSKKYNISKERIRQIETHAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHH
LKVKNFVISKRKEFGFHNI
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]