Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 58616836

GI number: 58616836

Start: 179785

End: 181416

Strand: Reverse

Name: pyrG

Synonym: ERGA_CDS_01090

Alternate gene names: 58616836

Gene position: 181416-179785 (Counterclockwise)

Preceding gene: 58616837

Following gene: 58616833

Centisome position: 12.1

GC content: 30.45

Gene sequence:

>1632_bases
ATGAACAATCTAACTTCAACTAAGTTTATTTTTGTGACTGGGGGTGTAGTGTCATCTCTAGGGAAAGGACTAGCAGCAGC
TAGTATAGGAGCATTATTACAGGCAAGAGGATTTAAGATTTGCTTAAGAAAATTAGATCCTTACTTGAACATAGATCCAG
GTACAATGAGCCCAATTCAGCATGGTGAAGTATTCGTAACTGATGATGGAGCAGAAACCGATTTAGACTTAGGTCACTAT
GAGCGTTTTACTGGTGTTAAAACAACAAAAAATGATAATATAACCACTGGAAAAGTATATCACAATTTATTAAACAAAGA
AAGAAAAGGAGATTATTTAGGACAAACAGTACAAATTATTCCTCATGTCACAGACCTAATTAACTCATTTATATTACATA
ATACTGATGCACTAGATTTTGTAATCTGTGAGATAGGTGGAACTGTAGGTGACATTGAAAGCCAGCCATTTCTTGAGTCT
ATTAGACAAATTGGTTATAAGCTTAGTAAAAATAATACAGTATTTGTACATCTAACTTTAGTTCCATATATTAGCGCAAC
TATGGAATTAAAAACTAAACCTACCCAACATTCGGTAAAAGAATTAAGTTCTGTAGGAATACAACCAGATATAATACTTT
ATCGTAGTAAAATACCTTTATCACAAGAACAACGAGATAAAATAGCTAACCTATGCAATGTTTCTCCAACAAATATTATC
CCTGCATTGGATGTTAAAAATATATATGAGCTACCTATCTCTTATCATCAATATAATCTAGATACACAAATATTAAAACA
TTTTAATATTACTTCTCCTGAACCAAATTTAGATAAATGGGAAAATATATTAAATATCTCTCATGTATCAACAAAAACAA
TTACTATTGCAATTATAGGAAAATATATCAAATTGTTAGATGCATATAAATCACTCATTGAAGCATTAGAACACGCAGCA
ATTCATAATAAAACTAAACTATCTATACACTGGATAGATTCACGCTCATTAAATAATGAAATTACTAATACTTTTGATAA
TGTCCATGCTATTTTAATACCAGGAGGTTTTGGTGACGATGGAGTAGAAGGAAAAATAATTGCTATTCAGTATGCACGTA
TCAACAACATACCATTTTTAGGAATTTGTATGGGTATGCAGCTTGCTATAATTGAATTTGCACGCAATGTTATACACCTT
GAAGATGCTAATTCTACAGAATTTAACTTTTATTGTAAAAATCCTGTTATACATCAATTACCAGAATTACAACAAAATCT
TGGCGGAAGCATGAAATTAGGGTCTCACCCGTGTTATTTAAAAGTAGATTCAAAAATTTTTTCTATATATAAAGAACAAG
TAATTAATGAAAGGAGAAGACATAGATATACTGTCAACTTACAATACAAAGACTTATTAGAAAGTCATGGTTTAATTTTT
ACTGGACACTCACACCATAACAATAATGATAGCTTAGCAGAAGTAATAGAACTAAAAAATCATCCATGGTTTATTGGAGT
ACAATTTCACCCAGAGTTTAAATCAGATCCTTTTCAATCTCATCCATTATTTATGTCATTTATACAAGCCTCCCTAAATT
ATCAAGAAACAAAACGGCATAAAATTTCATAA

Upstream 100 bases:

>100_bases
GTCTGTTGTGAAAAAGATTGCTGAAATGAGCAAGGTACCTACTGATCATCCCGCTGTAGACCATAATGAAGATAACATAC
CATTTAATCAATAAGGTTGT

Downstream 100 bases:

>100_bases
AGCTATATCCTTTTCTATATAAAAAATAGGCATATTATTATTTTATGACAAATACCTTATTGAAAATACATTATAATTAA
CTAGGATCATATCATAATAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MNNLTSTKFIFVTGGVVSSLGKGLAAASIGALLQARGFKICLRKLDPYLNIDPGTMSPIQHGEVFVTDDGAETDLDLGHY
ERFTGVKTTKNDNITTGKVYHNLLNKERKGDYLGQTVQIIPHVTDLINSFILHNTDALDFVICEIGGTVGDIESQPFLES
IRQIGYKLSKNNTVFVHLTLVPYISATMELKTKPTQHSVKELSSVGIQPDIILYRSKIPLSQEQRDKIANLCNVSPTNII
PALDVKNIYELPISYHQYNLDTQILKHFNITSPEPNLDKWENILNISHVSTKTITIAIIGKYIKLLDAYKSLIEALEHAA
IHNKTKLSIHWIDSRSLNNEITNTFDNVHAILIPGGFGDDGVEGKIIAIQYARINNIPFLGICMGMQLAIIEFARNVIHL
EDANSTEFNFYCKNPVIHQLPELQQNLGGSMKLGSHPCYLKVDSKIFSIYKEQVINERRRHRYTVNLQYKDLLESHGLIF
TGHSHHNNNDSLAEVIELKNHPWFIGVQFHPEFKSDPFQSHPLFMSFIQASLNYQETKRHKIS

Sequences:

>Translated_543_residues
MNNLTSTKFIFVTGGVVSSLGKGLAAASIGALLQARGFKICLRKLDPYLNIDPGTMSPIQHGEVFVTDDGAETDLDLGHY
ERFTGVKTTKNDNITTGKVYHNLLNKERKGDYLGQTVQIIPHVTDLINSFILHNTDALDFVICEIGGTVGDIESQPFLES
IRQIGYKLSKNNTVFVHLTLVPYISATMELKTKPTQHSVKELSSVGIQPDIILYRSKIPLSQEQRDKIANLCNVSPTNII
PALDVKNIYELPISYHQYNLDTQILKHFNITSPEPNLDKWENILNISHVSTKTITIAIIGKYIKLLDAYKSLIEALEHAA
IHNKTKLSIHWIDSRSLNNEITNTFDNVHAILIPGGFGDDGVEGKIIAIQYARINNIPFLGICMGMQLAIIEFARNVIHL
EDANSTEFNFYCKNPVIHQLPELQQNLGGSMKLGSHPCYLKVDSKIFSIYKEQVINERRRHRYTVNLQYKDLLESHGLIF
TGHSHHNNNDSLAEVIELKNHPWFIGVQFHPEFKSDPFQSHPLFMSFIQASLNYQETKRHKIS
>Mature_543_residues
MNNLTSTKFIFVTGGVVSSLGKGLAAASIGALLQARGFKICLRKLDPYLNIDPGTMSPIQHGEVFVTDDGAETDLDLGHY
ERFTGVKTTKNDNITTGKVYHNLLNKERKGDYLGQTVQIIPHVTDLINSFILHNTDALDFVICEIGGTVGDIESQPFLES
IRQIGYKLSKNNTVFVHLTLVPYISATMELKTKPTQHSVKELSSVGIQPDIILYRSKIPLSQEQRDKIANLCNVSPTNII
PALDVKNIYELPISYHQYNLDTQILKHFNITSPEPNLDKWENILNISHVSTKTITIAIIGKYIKLLDAYKSLIEALEHAA
IHNKTKLSIHWIDSRSLNNEITNTFDNVHAILIPGGFGDDGVEGKIIAIQYARINNIPFLGICMGMQLAIIEFARNVIHL
EDANSTEFNFYCKNPVIHQLPELQQNLGGSMKLGSHPCYLKVDSKIFSIYKEQVINERRRHRYTVNLQYKDLLESHGLIF
TGHSHHNNNDSLAEVIELKNHPWFIGVQFHPEFKSDPFQSHPLFMSFIQASLNYQETKRHKIS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=42.6497277676951, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=550, Percent_Identity=43.0909090909091, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=550, Percent_Identity=43.0909090909091, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=550, Percent_Identity=43.0909090909091, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=50.6422018348624, Blast_Score=557, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI25148299, Length=600, Percent_Identity=37.5, Blast_Score=412, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319432, Length=560, Percent_Identity=41.9642857142857, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322563, Length=562, Percent_Identity=38.7900355871886, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=551, Percent_Identity=41.3793103448276, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI21357815, Length=496, Percent_Identity=39.9193548387097, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_EHRRG (Q5FFC3)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196035.1
- HSSP:   P17812
- ProteinModelPortal:   Q5FFC3
- SMR:   Q5FFC3
- STRING:   Q5FFC3
- GeneID:   3268177
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_01090
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- BioCyc:   ERUM302409:ERGA_CDS_01090-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 61262; Mature: 61262

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 383-383 ACT_SITE 510-510 ACT_SITE 512-512

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLTSTKFIFVTGGVVSSLGKGLAAASIGALLQARGFKICLRKLDPYLNIDPGTMSPIQ
CCCCCCEEEEEEECHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCC
HGEVFVTDDGAETDLDLGHYERFTGVKTTKNDNITTGKVYHNLLNKERKGDYLGQTVQII
CCEEEEECCCCCCCCCCCHHHHHCCCEECCCCCCEEHHHHHHHHHHHHCCCHHCCHHHHH
PHVTDLINSFILHNTDALDFVICEIGGTVGDIESQPFLESIRQIGYKLSKNNTVFVHLTL
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCEEECCCCEEEEEEEE
VPYISATMELKTKPTQHSVKELSSVGIQPDIILYRSKIPLSQEQRDKIANLCNVSPTNII
ECHHCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
PALDVKNIYELPISYHQYNLDTQILKHFNITSPEPNLDKWENILNISHVSTKTITIAIIG
CCCCCCHHHHCCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCEEEEEHHH
KYIKLLDAYKSLIEALEHAAIHNKTKLSIHWIDSRSLNNEITNTFDNVHAILIPGGFGDD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHCCEEEEEEECCCCCC
GVEGKIIAIQYARINNIPFLGICMGMQLAIIEFARNVIHLEDANSTEFNFYCKNPVIHQL
CCCCEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCHHHHH
PELQQNLGGSMKLGSHPCYLKVDSKIFSIYKEQVINERRRHRYTVNLQYKDLLESHGLIF
HHHHHHCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHCCEEE
TGHSHHNNNDSLAEVIELKNHPWFIGVQFHPEFKSDPFQSHPLFMSFIQASLNYQETKRH
EECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHC
KIS
CCC
>Mature Secondary Structure
MNNLTSTKFIFVTGGVVSSLGKGLAAASIGALLQARGFKICLRKLDPYLNIDPGTMSPIQ
CCCCCCEEEEEEECHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCC
HGEVFVTDDGAETDLDLGHYERFTGVKTTKNDNITTGKVYHNLLNKERKGDYLGQTVQII
CCEEEEECCCCCCCCCCCHHHHHCCCEECCCCCCEEHHHHHHHHHHHHCCCHHCCHHHHH
PHVTDLINSFILHNTDALDFVICEIGGTVGDIESQPFLESIRQIGYKLSKNNTVFVHLTL
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCEEECCCCEEEEEEEE
VPYISATMELKTKPTQHSVKELSSVGIQPDIILYRSKIPLSQEQRDKIANLCNVSPTNII
ECHHCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
PALDVKNIYELPISYHQYNLDTQILKHFNITSPEPNLDKWENILNISHVSTKTITIAIIG
CCCCCCHHHHCCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCEEEEEHHH
KYIKLLDAYKSLIEALEHAAIHNKTKLSIHWIDSRSLNNEITNTFDNVHAILIPGGFGDD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHCCEEEEEEECCCCCC
GVEGKIIAIQYARINNIPFLGICMGMQLAIIEFARNVIHLEDANSTEFNFYCKNPVIHQL
CCCCEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCHHHHH
PELQQNLGGSMKLGSHPCYLKVDSKIFSIYKEQVINERRRHRYTVNLQYKDLLESHGLIF
HHHHHHCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHCCEEE
TGHSHHNNNDSLAEVIELKNHPWFIGVQFHPEFKSDPFQSHPLFMSFIQASLNYQETKRH
EECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHC
KIS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA