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The map label for this gene is grpE [H]

Identifier: 58616833

GI number: 58616833

Start: 174286

End: 174894

Strand: Reverse

Name: grpE [H]

Synonym: ERGA_CDS_01060

Alternate gene names: 58616833

Gene position: 174894-174286 (Counterclockwise)

Preceding gene: 58616836

Following gene: 58616832

Centisome position: 11.66

GC content: 31.2

Gene sequence:

>609_bases
TTGATAAGTATGACTGATAATACTAACAACAATACACAAAGCCCAGAAAATGATACTCCTAAACCTACTGAACAACAAAT
ACCTCCCAAAGCAAATCCACAAAGAAAATTTGCTTCAGAATTAAATAAAAAGAAAGAAAAGCTTAATGAAGATCTATCAG
AACTAGAAAAATTAAGACAACAATTAGCACATTTTCAACATCAGTTCCGCTTAGCAGTTGCTGATAAAGAAAACGTTAAG
CGCATCATGCAAAAAAATATAGATGAGGCATCTATTTATGCAATTTCCAACTTTGCTCGAGATATATTAACTTCTTGCGA
TAATTTAGAAACATCATTAGAAAATTTAAACAAAGATGACAGTATTCACGCTGGAGTACTAATGACTTATAAAGAATTAT
TAAATACCCTGGAGCGCCATAATATTAGTAGAATAGATCCAATTGGGGAACAGTTTAATCCACAATTTCACAAAGCTGTA
TCACAAATGATGGATACTGAAAAAGAAGATGGAACGATTTTACATGTAGTACAACCAGGTTATATAATAAAAGATAAATT
ATTACGCCCAGCATCAGTAGTTGTATCTAAAAAAAGTAATGGAGAGTAA

Upstream 100 bases:

>100_bases
ATCAACTAATAACTACAGAAAAACTATACATAACAAGCTCATCACCCTTGATATTTTCCTATAATACTACTATATATTGC
TTAGATTCTATAAATAATGT

Downstream 100 bases:

>100_bases
TTATCTGTGTCTACAATACAATAATTTCCATATAATATTTTTTATATGTGTATTAAAACACACGTTTTGTGTATCTGCAC
GTTTGACTAAGTAGCTATTT

Product: hypothetical protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MISMTDNTNNNTQSPENDTPKPTEQQIPPKANPQRKFASELNKKKEKLNEDLSELEKLRQQLAHFQHQFRLAVADKENVK
RIMQKNIDEASIYAISNFARDILTSCDNLETSLENLNKDDSIHAGVLMTYKELLNTLERHNISRIDPIGEQFNPQFHKAV
SQMMDTEKEDGTILHVVQPGYIIKDKLLRPASVVVSKKSNGE

Sequences:

>Translated_202_residues
MISMTDNTNNNTQSPENDTPKPTEQQIPPKANPQRKFASELNKKKEKLNEDLSELEKLRQQLAHFQHQFRLAVADKENVK
RIMQKNIDEASIYAISNFARDILTSCDNLETSLENLNKDDSIHAGVLMTYKELLNTLERHNISRIDPIGEQFNPQFHKAV
SQMMDTEKEDGTILHVVQPGYIIKDKLLRPASVVVSKKSNGE
>Mature_202_residues
MISMTDNTNNNTQSPENDTPKPTEQQIPPKANPQRKFASELNKKKEKLNEDLSELEKLRQQLAHFQHQFRLAVADKENVK
RIMQKNIDEASIYAISNFARDILTSCDNLETSLENLNKDDSIHAGVLMTYKELLNTLERHNISRIDPIGEQFNPQFHKAV
SQMMDTEKEDGTILHVVQPGYIIKDKLLRPASVVVSKKSNGE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=157, Percent_Identity=30.5732484076433, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI40255109, Length=155, Percent_Identity=24.5161290322581, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788967, Length=148, Percent_Identity=25.6756756756757, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17552458, Length=158, Percent_Identity=30.379746835443, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=167, Percent_Identity=34.1317365269461, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653432, Length=183, Percent_Identity=32.2404371584699, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 23082; Mature: 23082

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMTDNTNNNTQSPENDTPKPTEQQIPPKANPQRKFASELNKKKEKLNEDLSELEKLRQ
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAHFQHQFRLAVADKENVKRIMQKNIDEASIYAISNFARDILTSCDNLETSLENLNKDD
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SIHAGVLMTYKELLNTLERHNISRIDPIGEQFNPQFHKAVSQMMDTEKEDGTILHVVQPG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCEEEEECCC
YIIKDKLLRPASVVVSKKSNGE
EEHHHHHCCHHHHHEECCCCCC
>Mature Secondary Structure
MISMTDNTNNNTQSPENDTPKPTEQQIPPKANPQRKFASELNKKKEKLNEDLSELEKLRQ
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAHFQHQFRLAVADKENVKRIMQKNIDEASIYAISNFARDILTSCDNLETSLENLNKDD
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SIHAGVLMTYKELLNTLERHNISRIDPIGEQFNPQFHKAVSQMMDTEKEDGTILHVVQPG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCEEEEECCC
YIIKDKLLRPASVVVSKKSNGE
EEHHHHHCCHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA