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The map label for this gene is def [H]

Identifier: 58616771

GI number: 58616771

Start: 58496

End: 59041

Strand: Reverse

Name: def [H]

Synonym: ERGA_CDS_00440

Alternate gene names: 58616771

Gene position: 59041-58496 (Counterclockwise)

Preceding gene: 58616777

Following gene: 58616770

Centisome position: 3.94

GC content: 33.7

Gene sequence:

>546_bases
ATGTCTACTCTATCACTTGTTACCATTCCTGATCACAGATTATCACTATGCTCTGAAGAAGTTACAGAAGTAACTCAAGA
GATTAAGAAATTAGTTGATGATATGTTTGAAGTTATGCACACTAATAACGGAATAGGATTAGCTGCCGTACAAGTTGGAA
TCCACAAACGAATTTTTGTTGCTGATGTACCAGTAGATTACAAAGATCATGAAACAATAAAAACCGATGGTTATAAATCA
CATGGTGGACCATATTGCATGATTAATCCAAAAATTGTGGACATGTCTCAAGAAAAAGTAAAAATGCAAGAAGGATGCTT
ATCTGTACCAGATTGTTTAGAATATGTAATGCGTCCTAAATATGTTACAATGCAGTATCTAGACTATAATGGGAATAAGT
GTATAATAAAAGCTCAAGGCTGGCTTGCCAGATGTCTCGAACATGAGCTTGACCATTTAAACGGGATAGTCTTTTTGAAA
TATCTATCTAAGTTTAAGCGTGATCTTATAGTAGAGAAAATTAGAAAGAGACAGCCTATTAATTAA

Upstream 100 bases:

>100_bases
TAGTATGTAAAATAACATTGCTATAGTCGTTATATATTAATGCCTTATATATATGTTAATATTACAAAATAACAAATAGT
TTTTAATTTCGGGGGATCGA

Downstream 100 bases:

>100_bases
TTAAAAGTAGCGGTAAAATGTGTCAGATTCAGATTTACTTGAAGCACTAAAGTTTTTTTATGAAAACGGAGTAGATTGTA
TACTAGAAACTGAAAATCAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSTLSLVTIPDHRLSLCSEEVTEVTQEIKKLVDDMFEVMHTNNGIGLAAVQVGIHKRIFVADVPVDYKDHETIKTDGYKS
HGGPYCMINPKIVDMSQEKVKMQEGCLSVPDCLEYVMRPKYVTMQYLDYNGNKCIIKAQGWLARCLEHELDHLNGIVFLK
YLSKFKRDLIVEKIRKRQPIN

Sequences:

>Translated_181_residues
MSTLSLVTIPDHRLSLCSEEVTEVTQEIKKLVDDMFEVMHTNNGIGLAAVQVGIHKRIFVADVPVDYKDHETIKTDGYKS
HGGPYCMINPKIVDMSQEKVKMQEGCLSVPDCLEYVMRPKYVTMQYLDYNGNKCIIKAQGWLARCLEHELDHLNGIVFLK
YLSKFKRDLIVEKIRKRQPIN
>Mature_180_residues
STLSLVTIPDHRLSLCSEEVTEVTQEIKKLVDDMFEVMHTNNGIGLAAVQVGIHKRIFVADVPVDYKDHETIKTDGYKSH
GGPYCMINPKIVDMSQEKVKMQEGCLSVPDCLEYVMRPKYVTMQYLDYNGNKCIIKAQGWLARCLEHELDHLNGIVFLKY
LSKFKRDLIVEKIRKRQPIN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=136, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1789682, Length=176, Percent_Identity=40.9090909090909, Blast_Score=130, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24645726, Length=143, Percent_Identity=29.3706293706294, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24645728, Length=141, Percent_Identity=30.4964539007092, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20817; Mature: 20685

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLSLVTIPDHRLSLCSEEVTEVTQEIKKLVDDMFEVMHTNNGIGLAAVQVGIHKRIFV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCEEEE
ADVPVDYKDHETIKTDGYKSHGGPYCMINPKIVDMSQEKVKMQEGCLSVPDCLEYVMRPK
EECCCCCCCCCCEECCCCCCCCCCEEEECCEEECCCHHHHHHHHCCCCHHHHHHHHHCCC
YVTMQYLDYNGNKCIIKAQGWLARCLEHELDHLNGIVFLKYLSKFKRDLIVEKIRKRQPI
EEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
N
C
>Mature Secondary Structure 
STLSLVTIPDHRLSLCSEEVTEVTQEIKKLVDDMFEVMHTNNGIGLAAVQVGIHKRIFV
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCEEEE
ADVPVDYKDHETIKTDGYKSHGGPYCMINPKIVDMSQEKVKMQEGCLSVPDCLEYVMRPK
EECCCCCCCCCCEECCCCCCCCCCEEEECCEEECCCHHHHHHHHCCCCHHHHHHHHHCCC
YVTMQYLDYNGNKCIIKAQGWLARCLEHELDHLNGIVFLKYLSKFKRDLIVEKIRKRQPI
EEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA