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The map label for this gene is ruvC

Identifier: 58616730

GI number: 58616730

Start: 1552

End: 2034

Strand: Reverse

Name: ruvC

Synonym: ERGA_CDS_00030

Alternate gene names: 58616730

Gene position: 2034-1552 (Counterclockwise)

Preceding gene: 58616731

Following gene: 58616728

Centisome position: 0.14

GC content: 30.23

Gene sequence:

>483_bases
ATGAACATAATAGGTATTGATCCAAGCTTAAATTCCACAGGATGGGCTATACTGTCTGTAAATGATAACAACTATAATGA
AATTCGTTTGGTTAATAATGGTTCTATATTAACAAGCAACAAGAAAACTATTGGTGAAAGGCTAAACAAAATATACAGTG
AATTATTGAACATACTTAACTCTTATAAAGTAGACACTGCATCTATGGAAGAGATCTTTATAAATAAAAATCCAAAATCT
TCAACATTGCTATGCTATGCCAGAGGTATACTACTACTAACTTTAGATGTCGCTTGTATACCATTATTTGAATACTCAGC
AAATCGTGTAAAAAAATCTATTACAGGAAATGGACACGCCAAAAAGGAACAAGTATGCTTTATGATCGAAAATATATTAA
ATATTAAATGCTATGGTACTTACGATATATCTGATGCTATAGCAGTAGCTATATGTCACATCTATTCCATAAAAGCATTT
TGA

Upstream 100 bases:

>100_bases
GACTATAAAAAATAAAAAGCCACTCTTGTTTTTACACACAAGTATTCTATAAATTATGAAAATATACCATAGCAAAAGCA
ATAATTAGCATTGAAATACT

Downstream 100 bases:

>100_bases
AAAAATATCATAGCTTTAAATTTATTTTATTTTCACTTTTATTTAACATCCGTATAATGTTATCAGTATGCTGTGTTATT
ATTAGTAATGCTGTGATTGC

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MNIIGIDPSLNSTGWAILSVNDNNYNEIRLVNNGSILTSNKKTIGERLNKIYSELLNILNSYKVDTASMEEIFINKNPKS
STLLCYARGILLLTLDVACIPLFEYSANRVKKSITGNGHAKKEQVCFMIENILNIKCYGTYDISDAIAVAICHIYSIKAF

Sequences:

>Translated_160_residues
MNIIGIDPSLNSTGWAILSVNDNNYNEIRLVNNGSILTSNKKTIGERLNKIYSELLNILNSYKVDTASMEEIFINKNPKS
STLLCYARGILLLTLDVACIPLFEYSANRVKKSITGNGHAKKEQVCFMIENILNIKCYGTYDISDAIAVAICHIYSIKAF
>Mature_160_residues
MNIIGIDPSLNSTGWAILSVNDNNYNEIRLVNNGSILTSNKKTIGERLNKIYSELLNILNSYKVDTASMEEIFINKNPKS
STLLCYARGILLLTLDVACIPLFEYSANRVKKSITGNGHAKKEQVCFMIENILNIKCYGTYDISDAIAVAICHIYSIKAF

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=135, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_EHRRG (Q5FF10)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_195929.1
- ProteinModelPortal:   Q5FF10
- SMR:   Q5FF10
- STRING:   Q5FF10
- GeneID:   3268331
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_00030
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   CLSK749295
- BioCyc:   ERUM302409:ERGA_CDS_00030-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17831; Mature: 17831

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIGIDPSLNSTGWAILSVNDNNYNEIRLVNNGSILTSNKKTIGERLNKIYSELLNILN
CEEEECCCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHH
SYKVDTASMEEIFINKNPKSSTLLCYARGILLLTLDVACIPLFEYSANRVKKSITGNGHA
HCCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCC
KKEQVCFMIENILNIKCYGTYDISDAIAVAICHIYSIKAF
CHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIIGIDPSLNSTGWAILSVNDNNYNEIRLVNNGSILTSNKKTIGERLNKIYSELLNILN
CEEEECCCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHH
SYKVDTASMEEIFINKNPKSSTLLCYARGILLLTLDVACIPLFEYSANRVKKSITGNGHA
HCCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCC
KKEQVCFMIENILNIKCYGTYDISDAIAVAICHIYSIKAF
CHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA