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The map label for this gene is purN

Identifier: 57651681

GI number: 57651681

Start: 1090828

End: 1091394

Strand: Direct

Name: purN

Synonym: SACOL1081

Alternate gene names: 57651681

Gene position: 1090828-1091394 (Clockwise)

Preceding gene: 57651680

Following gene: 57651682

Centisome position: 38.83

GC content: 32.45

Gene sequence:

>567_bases
ATGGTTAAAATTGCGATTTTTGCATCAGGTTCAGGAAGTAACTTTGAAAATATAGTTGAGCATGTTGAATCAGGAAAACT
TGAAAATATTGAAGTTACGGCGCTATATACGGATCATCAAAATGCGTTTTGTATAGATAGAGCAAAAAAGCACGATATTC
CTGTTTATATTAATGAACCAAAACAATTTGATTCAAAAGCAGCGTATGAACAACATTTAGTAACATTATTAAATGAAGAT
AAGGTAGAGTGGATTATTCTAGCTGGCTACATGCGTCTAATTGGTCCAGACTTATTAGCTTCATTTGAAGGTAAAATATT
GAATATACATCCATCTCTATTGCCGAAATATAAGGGGATTGACGCAATAGGCCAAGCATATCATAGTGGCGATACTATTA
CTGGTTCGACAGTACATTATGTTGATAGTGGTATGGATACGGGAGAAATTATTGAACAGAGAAAATGTGATATTAGACCG
GACGATTCAAAAGAACAATTAGAAGAGAAAGTAAAAAAATTGGAATATGAGTTATATCCAAGTGTTATTGCTAAAATTGT
AAAATAA

Upstream 100 bases:

>100_bases
GCGCTTTGAAGATTTTAGCAGAACAAAATGTGGAAGCCTATCAAATTGGTCATATTGTGAAAAATGAGTCAACTGCAATT
GAATTGTTGGGGGTATAACA

Downstream 100 bases:

>100_bases
GGAGTATGTATGTGATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCGTTAACG
CAATTAAATTATGAATTATA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFCIDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNED
KVEWIILAGYMRLIGPDLLASFEGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
DDSKEQLEEKVKKLEYELYPSVIAKIVK

Sequences:

>Translated_188_residues
MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFCIDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNED
KVEWIILAGYMRLIGPDLLASFEGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
DDSKEQLEEKVKKLEYELYPSVIAKIVK
>Mature_188_residues
MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFCIDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNED
KVEWIILAGYMRLIGPDLLASFEGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
DDSKEQLEEKVKKLEYELYPSVIAKIVK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family

Homologues:

Organism=Homo sapiens, GI4503915, Length=181, Percent_Identity=29.8342541436464, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI209869995, Length=181, Percent_Identity=29.8342541436464, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI209869993, Length=181, Percent_Identity=29.8342541436464, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1788846, Length=183, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1787483, Length=151, Percent_Identity=28.476821192053, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=191, Percent_Identity=32.4607329842932, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=26.7676767676768, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=33.8709677419355, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR3_STAAC (Q5HH12)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185945.1
- ProteinModelPortal:   Q5HH12
- SMR:   Q5HH12
- STRING:   Q5HH12
- EnsemblBacteria:   EBSTAT00000010825
- GeneID:   3236105
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1081
- TIGR:   SACOL1081
- eggNOG:   COG0299
- GeneTree:   EBGT00050000024715
- HOGENOM:   HBG649382
- OMA:   AGDSEHG
- ProtClustDB:   CLSK885118
- BioCyc:   SAUR93062:SACOL1081-MONOMER
- InterPro:   IPR002376
- InterPro:   IPR004607
- Gene3D:   G3DSA:3.40.50.170
- TIGRFAMs:   TIGR00639

Pfam domain/function: PF00551 Formyl_trans_N; SSF53328 formyl_transf

EC number: =2.1.2.2

Molecular weight: Translated: 21151; Mature: 21151

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00373 GART

Important sites: ACT_SITE 110-110 BINDING 66-66 BINDING 108-108 BINDING 146-146 BINDING 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFCIDRAKKHDIPVYINEP
CEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCCCCCEEECCC
KQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASFEGKILNIHPSLLPKYKGI
HHCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCHHHHHHCCCCEEEECHHHCCCCCCH
DAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRPDDSKEQLEEKVKKLEYELYP
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
SVIAKIVK
HHHHHHCC
>Mature Secondary Structure
MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFCIDRAKKHDIPVYINEP
CEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCCCCCEEECCC
KQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASFEGKILNIHPSLLPKYKGI
HHCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCHHHHHHCCCCEEEECHHHCCCCCCH
DAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRPDDSKEQLEEKVKKLEYELYP
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
SVIAKIVK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA