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The map label for this gene is purF

Identifier: 57651679

GI number: 57651679

Start: 1088320

End: 1089804

Strand: Direct

Name: purF

Synonym: SACOL1079

Alternate gene names: 57651679

Gene position: 1088320-1089804 (Clockwise)

Preceding gene: 57651678

Following gene: 57651680

Centisome position: 38.74

GC content: 35.96

Gene sequence:

>1485_bases
ATGTTTAACTACTCAGGATTAAACGAAGAATGTGGCGTGTTTGGTATTTGGAATCATCCTGAAGCAGCGCAACTAACATA
TATGGGACTTCATAGTTTGCAACATCGTGGTCAAGAAGGTGCAGGTATAGTTGTTTCTGATCAAAATGAATTAAAAGGCG
AGCGAGGATTAGGCTTACTAACTGAAGCGATTAAAGATGATCAAATGGAACGATTAAAAGGATATCAACATGCAATTGGT
CACGTCCGTTATGCTACTTCAGGTAATAAAGGTATTGAAAATATTCAACCGTTTCTGTATCACTTTTATGATATGAGTGT
AGGTATTTGTCATAATGGTAACCTCATTAATGCTAAATCATTGCGTCAGAATTTAGAAAAACAAGGTGCTATCTTCCATT
CGTCTTCTGATACTGAAGTCATTATGCATTTGATACGTCGAAGTAAAGCTCCTACTTTTGAGGAAGCGTTGAAAGAAAGT
TTGCGAAAAGTTAAAGGCGGTTTTACATTTGCGATTTTAACTAAAGATGCGTTATATGGCGCAGTAGATCCAAATGCTAT
CAGACCACTTGTTGTAGGTAAAATGAAAGATGGGACATACATCCTTGCAAGTGAAACATGTGCAATAGATGTGTTAGGTG
CAGAATTTGTTCAAGATATTCATGCAGGTGAATATGTCGTGATTAACGATAAAGGTATTACAGTTAAATCTTATACACAT
CATACGACAACTGCAATTTCTGCGATGGAATATATTTATTTTGCTAGACCAGACTCAACAATAGCTGGTAAAAATGTCCA
TGCAGTACGTAAAGCTTCTGGTAAAAAATTAGCCCAAGAAAGCCCTGTAAATGCTGATATGGTCATCGGTGTACCCAATT
CATCGCTATCAGCTGCGAGTGGTTATGCTGAAGAAATAGGTTTGCCATATGAAATGGGACTAGTTAAAAATCAATATGTT
GCAAGAACATTTATTCAACCAACTCAAGAATTACGTGAGCAAGGTGTGAGAGTGAAGTTATCTGCGGTAAAAGATATAGT
AGATGGGAAAAATATCATTCTTGTTGATGATTCCATTGTTCGCGGTACGACAATTCGACGCATTGTGAAAATGTTAAAAG
ATTCTGGTGCAAATAAAGTGCATGTGCGTATAGCATCACCGGAATTTATGTTTCCAAGTTTTTACGGAATCGATGTTTCA
ACTACGGCAGAATTAATTTCTGCAAGCAAATCACCTGAAGAAATTAAAGATTATATTGGCGCTGATTCATTAGCATATCT
ATCTGTAGATGGGTTAATTGAATCAATTGGTTTAGATTATGACGCGCCATATAGTGGCTTATGTGTAGAAAGTTTCACTG
GAGATTATCCTGCAGGGTTATATGATTATGAAGCAAATTATAAAGCGCATTTAAGTCATCGACAAAAGCAATATATTTCT
AAAAACAAACACTTTTTTGATAGCGAGGGAAATTTAAATGTCTAA

Upstream 100 bases:

>100_bases
TTTTAACAGATAGTGATAATTTCAAGGTAACAACACCATATACAGAGATTAGTGAAAATGTTTCAGATATTAAACAAATA
TGGGAAGGGGCAATTGCTCA

Downstream 100 bases:

>100_bases
AGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAAAGAATGTCTAGTCATGTTAAACGTACGA
TGCGTAAAGAAGTTATCGGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV

Sequences:

>Translated_494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV
>Mature_494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI29570798, Length=510, Percent_Identity=36.6666666666667, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI4826742, Length=209, Percent_Identity=29.1866028708134, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788651, Length=448, Percent_Identity=36.3839285714286, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1790167, Length=216, Percent_Identity=28.2407407407407, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=39.7489539748954, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=34.5755693581781, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=37.2233400402414, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=36.5853658536585, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=36.6952789699571, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI21357745, Length=207, Percent_Identity=29.4685990338164, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR1_STAAC (Q5HH14)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185943.1
- ProteinModelPortal:   Q5HH14
- SMR:   Q5HH14
- STRING:   Q5HH14
- EnsemblBacteria:   EBSTAT00000008951
- GeneID:   3236103
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1079
- TIGR:   SACOL1079
- eggNOG:   COG0034
- GeneTree:   EBGT00050000023831
- HOGENOM:   HBG392416
- OMA:   VWAPGEE
- ProtClustDB:   PRK07272
- BioCyc:   SAUR93062:SACOL1079-MONOMER
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836
- PANTHER:   PTHR11907
- PIRSF:   PIRSF000485
- TIGRFAMs:   TIGR01134

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran

EC number: =2.4.2.14

Molecular weight: Translated: 54397; Mature: 54397

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS51278 GATASE_TYPE_2; PS00103 PUR_PYR_PR_TRANSFER; PS00443 GATASE_TYPE_II

Important sites: ACT_SITE 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLL
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCHHH
TEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKS
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCEECHHH
LRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYG
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHCC
AVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
CCCCCCCCCEEEEEECCCCEEEEECCEEEEEHHHHHHHHHCCCCEEEECCCCEEEEECCC
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAAS
HHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCHHC
GYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIV
CCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHH
RGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIG
CCHHHHHHHHHHHCCCCCEEEEEECCCCEECCCEECCCHHHHHHHHHCCCCHHHHHHHHC
ADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
CCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
KNKHFFDSEGNLNV
CCCCEECCCCCCCC
>Mature Secondary Structure
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLL
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCHHH
TEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKS
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCEECHHH
LRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYG
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHCC
AVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
CCCCCCCCCEEEEEECCCCEEEEECCEEEEEHHHHHHHHHCCCCEEEECCCCEEEEECCC
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAAS
HHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCHHC
GYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIV
CCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHH
RGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIG
CCHHHHHHHHHHHCCCCCEEEEEECCCCEECCCEECCCHHHHHHHHHCCCCHHHHHHHHC
ADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
CCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
KNKHFFDSEGNLNV
CCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA