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The map label for this gene is adhE [H]

Identifier: 57651152

GI number: 57651152

Start: 150451

End: 153060

Strand: Direct

Name: adhE [H]

Synonym: SACOL0135

Alternate gene names: 57651152

Gene position: 150451-153060 (Clockwise)

Preceding gene: 57651151

Following gene: 57651153

Centisome position: 5.36

GC content: 36.13

Gene sequence:

>2610_bases
ATGTTAACTATACCTGAAAAAGAAAATCGTGGATCGAAAGAACAAGAAGTGGCAATTATGATTGATGCTCTAGCTGACAA
AGGGAAAAAAGCATTAGAAGCATTATCTAAAAAGTCACAAGAAGAAATTGATCATATTGTTCATCAAATGAGCTTAGCAG
CTGTTGATCAACATATGGTGCTAGCAAAATTAGCACATGAAGAAACTGGAAGAGGTATATACGAAGATAAAGCGATTAAA
AATTTATACGCTTCTGAATATATATGGAATTCAATAAAAGACAATAAGACAGTAGGGATTATTGGTGAAGATAAAGAAAA
AGGATTAACGTATGTAGCGGAACCAATTGGTGTTATTTGTGGTGTTACGCCAACAACAAATCCTACGTCGACAACTATTT
TTAAAGCGATGATTGCAATTAAGACAGGAAATCCAATCATTTTTGCATTCCATCCAAGTGCACAAGAATCGTCGAAGCGT
GCAGCAGAAGTTGTATTAGAAGCGGCAATGAAGGCAGGTGCACCTAAAGATATTATTCAGTGGATTGAAGTGCCTTCTAT
CGAAGCAACAAAACAATTAATGAATCACAAAGGTATTGCATTAGTTCTAGCAACAGGTGGTTCGGGCATGGTTAAGTCTG
CATATTCAACTGGCAAACCGGCATTAGGTGTGGGACCAGGTAACGTGCCGTCTTACATTGAAAAAACAGCACACATTAAA
CGTGCAGTAAATGATATCATTGGTTCAAAAACATTTGATAATGGTATGATTTGTGCTTCTGAACAAGTTGTAGTCATTGA
TAAAGAAATTTATAAAGATGTTACTAATGAATTTAAAGCACATCAAGCATACTTTGTTAAAAAAGATGAATTACAACGCT
TAGAAAATGCAATTATGAATGAACAAAAAACAGGTATTAAGCCTGATATTGTCGGTAAATCTGCAGTTGAAATAGCTGAA
TTAGCAGGTATACCTGTCCCCGAAAATACAAAACTTATCATAGCCGAAATTAGCGGTGTAGGTTCAGACTATCCGTTATC
TCGTGAAAAATTATCTCCAGTATTAGCCTTAGTAAAAGCCCAATCTACAAAACAAGCATTTCAAATTTGTGAAGACACAC
TACATTTTGGTGGATTAGGACACACAGCCGTTATCCATACAGAAGATGAAACATTACAAAAAGATTTTGGACTAAGAATG
AAAGCTTGTCGTGTACTTGTAAATACACCATCAGCGGTTGGAGGTATTGGTGATATGTATAACGAATTGATTCCGTCTTT
AACATTAGGTTGTGGTTCCTACGGTAGAAACTCAATTTCACATAATGTTAGTGCGACAGATTTATTAAACATTAAAACGA
TTGCTAAACGACGTAATAATACTCAAATTTTCAAGGTGCCTGCTCAAATTTATTTTGAAGAAAATGCAATCATGAGTCTA
ACAACAATGGACAAGATTGAAAAAGTGATGATTGTCTGTGACCCTGGTATGGTAGAATTCGGTTATACAAAAACAGTTGA
GAATGTATTAAGACAAAAAACGGAACAGCCTCAAATTAAAATATTTAGCGAAGTCGAACCGAACCCATCAACTAATACAG
TATATAAAGGTCTGGAAATGATGGTTGATTTCCAACCGGATACAATCATTGCACTTGGTGGTGGTTCAGCGATGGATGCT
GCAAAAGCAATGTGGATGTTCTTTGAACACCCTGAGACATCATTCTTCGGTGCTAAACAAAAGTTCCTAGACATCGGTAA
ACGTACTTATAAAATAGGCATGCCTGAAAATGCGACGTTCATTTGTATCCCTACGACATCAGGTACAGGTTCAGAAGTAA
CACCATTTGCAGTTATCACAGATAGTGAAACAAATGTAAAATATCCGTTGGCTGATTTTGCTTTAACACCTGACGTTGCA
ATTATTGACCCTCAATTTGTGATGAGTGTGCCAAAAAGCGTTACAGCAGATACAGGAATGGATGTACTAACGCATGCAAT
GGAATCATATGTATCTGTAATGGCTTCAGACTACACAAGAGGTTTGAGTCTACAAGCGATTAAATTGACGTTCGAATATT
TAAAATCATCTGTTGAAAAGGGTGATAAAGTTTCAAGAGAGAAAATGCATAACGCATCAACTTTGGCTGGTATGGCATTT
GCAAATGCATTCTTAGGCATTGCACACTCAATTGCGCATAAAATTGGTGGCGAATATGGTATTCCGCATGGTAGAGCGAA
TGCGATATTACTACCGCATATTATCCGTTATAATGCCAAAGACCCGCAAAAACATGCATTATTCCCTAAATATGAGTTCT
TCAGAGCAGATACAGATTATGCAGATATTGCCAAATTCTTAGGATTAAAAGGTAATACGACAGAAGCACTCGTAGAATCA
TTAGCTAAAGCTGTCTACGAATTAGGTCAATCAGTCGGAATTGAAATGAATTTGAAATCACAAGGTGTGTCTGAAGAAGA
ATTAAATGAGTCAATTGATAGAATGGCAGAGCTCGCATTTGAAGATCAATGTACAACTGCTAATCCTAAAGAAGCACTAA
TCAGTGAAATCAAAGATATCATTCAAACATCATATGATTATAAGCAATAA

Upstream 100 bases:

>100_bases
CTAAATTAAACTTTTTTGTATAGAATTTTCTTTATATCCTGAGAGACATGTACTATAATGTTTGTGAAATAATTCACAAA
GTATAAAGGAGTGGTTGTAT

Downstream 100 bases:

>100_bases
TCTATCTGATAATAATCATCTAACTCACCTGAAATTACAAAAGTAAAAAATGCCACATAAACTTTAAGTCGATAATCATT
ATACGGTTATCGGCTTTTAT

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 869; Mature: 869

Protein sequence:

>869_residues
MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIK
NLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTAHIK
RAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAE
LAGIPVPENTKLIIAEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLRM
KACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRRNNTQIFKVPAQIYFEENAIMSL
TTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDA
AKAMWMFFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPDVA
IIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAF
ANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALVES
LAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISEIKDIIQTSYDYKQ

Sequences:

>Translated_869_residues
MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIK
NLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTAHIK
RAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAE
LAGIPVPENTKLIIAEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLRM
KACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRRNNTQIFKVPAQIYFEENAIMSL
TTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDA
AKAMWMFFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPDVA
IIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAF
ANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALVES
LAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISEIKDIIQTSYDYKQ
>Mature_869_residues
MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIK
NLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTAHIK
RAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAE
LAGIPVPENTKLIIAEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLRM
KACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRRNNTQIFKVPAQIYFEENAIMSL
TTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDA
AKAMWMFFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPDVA
IIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAF
ANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALVES
LAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISEIKDIIQTSYDYKQ

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=328, Percent_Identity=27.1341463414634, Blast_Score=95, Evalue=4e-19,
Organism=Escherichia coli, GI1787493, Length=858, Percent_Identity=48.8344988344988, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1789163, Length=403, Percent_Identity=34.7394540942928, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI48994951, Length=384, Percent_Identity=33.8541666666667, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87082107, Length=330, Percent_Identity=33.6363636363636, Blast_Score=166, Evalue=7e-42,
Organism=Escherichia coli, GI1788797, Length=355, Percent_Identity=31.2676056338028, Blast_Score=164, Evalue=3e-41,
Organism=Escherichia coli, GI1789386, Length=321, Percent_Identity=24.6105919003115, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17537053, Length=265, Percent_Identity=29.4339622641509, Blast_Score=108, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321181, Length=410, Percent_Identity=32.4390243902439, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24657991, Length=381, Percent_Identity=26.7716535433071, Blast_Score=95, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 94946; Mature: 94946

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMV
CCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVIC
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHCCHHHEE
GVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKRAAEVVLEAAMKAGAPKDIIQ
ECCCCCCCCHHHHHHEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
WIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTAHIK
HHCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLENAIMN
HHHHHHHCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHC
EQKTGIKPDIVGKSAVEIAELAGIPVPENTKLIIAEISGVGSDYPLSREKLSPVLALVKA
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
QSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLRMKACRVLVNTPSAVGGIGDMY
HHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHCCCCCHHCCHHHHH
NELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRRNNTQIFKVPAQIYFEENAIMSL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCEEEE
TTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEM
HHHHHCCEEEEEECCCCEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHH
MVDFQPDTIIALGGGSAMDAAKAMWMFFEHPETSFFGAKQKFLDIGKRTYKIGMPENATF
EEECCCCEEEEECCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCEEEECCCCCCEE
ICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGM
EEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEECCHHHHHCCCHHCCCCCH
DVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAF
HHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDY
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHCCCCCCHHHCCCCCHHHEECCCCH
ADIAKFLGLKGNTTEALVESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQCTTANPKEALISEIKDIIQTSYDYKQ
CCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMV
CCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVIC
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHCCHHHEE
GVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKRAAEVVLEAAMKAGAPKDIIQ
ECCCCCCCCHHHHHHEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
WIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTAHIK
HHCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLENAIMN
HHHHHHHCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHC
EQKTGIKPDIVGKSAVEIAELAGIPVPENTKLIIAEISGVGSDYPLSREKLSPVLALVKA
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
QSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLRMKACRVLVNTPSAVGGIGDMY
HHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHCCCCCHHCCHHHHH
NELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRRNNTQIFKVPAQIYFEENAIMSL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCEEEE
TTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEM
HHHHHCCEEEEEECCCCEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHH
MVDFQPDTIIALGGGSAMDAAKAMWMFFEHPETSFFGAKQKFLDIGKRTYKIGMPENATF
EEECCCCEEEEECCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCEEEECCCCCCEE
ICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGM
EEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEECCHHHHHCCCHHCCCCCH
DVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAF
HHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDY
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHCCCCCCHHHCCCCCHHHEECCCCH
ADIAKFLGLKGNTTEALVESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQCTTANPKEALISEIKDIIQTSYDYKQ
CCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]