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The map label for this gene is yfkN [H]

Identifier: 57651151

GI number: 57651151

Start: 145801

End: 147336

Strand: Direct

Name: yfkN [H]

Synonym: SACOL0130

Alternate gene names: 57651151

Gene position: 145801-147336 (Clockwise)

Preceding gene: 57651150

Following gene: 57651152

Centisome position: 5.19

GC content: 36.33

Gene sequence:

>1536_bases
ATGTCAAACATAGCATTTTATGTCGTGAGTGACGTACATGGTTATATTTTCCCAACAGATTTTACGAGTAGAAATCAATA
TCAACCTATGGGATTGTTACTAGCGAATCATGTTATAGAACAAGACAGAAGGCAGTATGACCAAAGTTTTAAAATAGATA
ATGGTGATTTTTTGCAAGGGTCACCATTTTGTAATTACTTAATCGCGCATAGCGGCAGTAGCCAGCCTTTAGTTGATTTT
TATAATCGAATGGCATTCGACTTTGGTACGCTTGGTAATCATGAATTTAATTATGGATTACCATACTTAAAAGACACTTT
ACGCAGACTCAATTATCCAGTTTTGTGCGCTAATATATATGAAAATGATAGTACATTGACTGATAACGGTGTGAAGTATT
TTCAGGTTGGAGATCAAACTGTTGGTGTGATAGGTTTAACGACACAATTTATTCCCCATTGGGAACAACCAGAGCATATT
CAGTCACTTACGTTTCATAGTGCTTTTGAAATACTTCAACAATACTTACCTGAAATGAAGCGACATGCAGATATCATTGT
GGTTTGTTACCATGGTGGATTTGAAAAGGATTTAGAAAGTGGTACGCCGACCGAAGTATTAACGGGTGAAAATGAAGGAT
ATGCCATGTTAGAAGCGTTTTCTAAAGATATAGATATCTTTATTACGGGTCACCAACATCGACAAATTGCTGAAAGGTTT
AAGCAAACGGCTGTGATTCAACCTGGTACGAGAGGTACAACTGTAGGCAGAGTAGTCTTGAGTACTGATGAATATGAAAA
TCTATCCGTTGAATCATGTGAATTACTTCCTGTTATAGATGATTCCACATTTACTATTGATGAAGATGACCAACATTTAC
GAAAGCAGTTAGAGGACTGGTTAGATTACGAAATTACTACATTGCCATATGATATGACGATTAATCATGCATTTGAGGCA
CGTGTGGCACCGCATCCTTTTACAAATTTTATGAATTACGCTTTATTAGAAAAAAGTGACGCAGATGTTGCCTGTACAGC
TTTGTTTGATTCTGCTAGTGGTTTCAAGCAAGTCGTGACGATGCGAGATGTTATTAACAATTACCCATTTCCAAATACAT
TTAAAGTTTTAGCTGTAAGTGGTGCCAAACTTAAAGAAGCCATTGAACGATCAGCAGAATATTTTGACGTGAAAAATGAT
GAAGTTAGTGTGAGCGCAGACTTCCTTGAACCCAAACCACAACACTTTAATTATGATATATATGGTGGCGTAAGTTATAC
CATTCATGTTGGAAGACCAAAGGGACAACGTGTGAGCAATATGATGATTCAAGGTCACGCAGTTGATTTAAAGCAGACAT
ATACAATTTGTGTAAATAATTATCGTGCAGTAGGCGGTGGTCAGTATGATATGTATATCGACGCGCCAGTTGTAAAAGAT
ATTCAAGTTGAAGGCGCACAATTACTTATTGATTTTTTATCAAATAATAATTTGATGCGCATCCCGCAAGTTGTTGATTT
TAAAGTTGAAAAGTGA

Upstream 100 bases:

>100_bases
TTCGTTTAAAACTCGGTGACAAAACCTTGATTACAACAGACTTCAAATAAATATATAATAGAAATAAGAAAGATGTTTGT
GATTGAAGGAGTGAGTGAGG

Downstream 100 bases:

>100_bases
CGGATATATGTAAAACTTACTTGATTTTAATCGGGTAAGTTTTTGTTTTTGTTTAAAGCGAGAAAGTATTATCTTTAGAC
GACGTGATTGAAATGTCACG

Product: 5' nucleotidase family protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRRQYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDF
YNRMAFDFGTLGNHEFNYGLPYLKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLEAFSKDIDIFITGHQHRQIAERF
KQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELLPVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEA
RVAPHPFTNFMNYALLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSAEYFDVKND
EVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDLKQTYTICVNNYRAVGGGQYDMYIDAPVVKD
IQVEGAQLLIDFLSNNNLMRIPQVVDFKVEK

Sequences:

>Translated_511_residues
MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRRQYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDF
YNRMAFDFGTLGNHEFNYGLPYLKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLEAFSKDIDIFITGHQHRQIAERF
KQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELLPVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEA
RVAPHPFTNFMNYALLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSAEYFDVKND
EVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDLKQTYTICVNNYRAVGGGQYDMYIDAPVVKD
IQVEGAQLLIDFLSNNNLMRIPQVVDFKVEK
>Mature_510_residues
SNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRRQYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFY
NRMAFDFGTLGNHEFNYGLPYLKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHIQ
SLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLEAFSKDIDIFITGHQHRQIAERFK
QTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELLPVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEAR
VAPHPFTNFMNYALLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSAEYFDVKNDE
VSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDLKQTYTICVNNYRAVGGGQYDMYIDAPVVKDI
QVEGAQLLIDFLSNNNLMRIPQVVDFKVEK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=538, Percent_Identity=26.0223048327138, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 58111; Mature: 57980

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRRQYDQSFKIDNGDFLQG
CCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPYLKDTLRRLNYPVLCANIY
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
ENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHIQSLTFHSAFEILQQYLPEMK
CCCCEECCCCEEEEEECCCEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLEAFSKDIDIFITGHQHRQIAERF
CCCCEEEEEECCCCHHHHCCCCCCEEEECCCCCCHHHHHHCCCEEEEEECCHHHHHHHHH
KQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELLPVIDDSTFTIDEDDQHLRKQLEDW
HHCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHH
LDYEITTLPYDMTINHAFEARVAPHPFTNFMNYALLEKSDADVACTALFDSASGFKQVVT
HCCEEEEEEEEEEECCEEECCCCCCCHHHHHHHHEEECCCCCEEEEEEECCCCHHHHHHH
MRDVINNYPFPNTFKVLAVSGAKLKEAIERSAEYFDVKNDEVSVSADFLEPKPQHFNYDI
HHHHHHCCCCCCCEEEEEECCHHHHHHHHCCHHHEECCCCCEEEEECCCCCCCCCCCEEE
YGGVSYTIHVGRPKGQRVSNMMIQGHAVDLKQTYTICVNNYRAVGGGQYDMYIDAPVVKD
ECCEEEEEEECCCCCCHHHHEEEECEEECCCEEEEEEEECEEECCCCEEEEEECCCCEEC
IQVEGAQLLIDFLSNNNLMRIPQVVDFKVEK
EECCCHHHHHHHHCCCCEEECCCEECEEECC
>Mature Secondary Structure 
SNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRRQYDQSFKIDNGDFLQG
CCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPYLKDTLRRLNYPVLCANIY
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
ENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHIQSLTFHSAFEILQQYLPEMK
CCCCEECCCCEEEEEECCCEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLEAFSKDIDIFITGHQHRQIAERF
CCCCEEEEEECCCCHHHHCCCCCCEEEECCCCCCHHHHHHCCCEEEEEECCHHHHHHHHH
KQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELLPVIDDSTFTIDEDDQHLRKQLEDW
HHCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHH
LDYEITTLPYDMTINHAFEARVAPHPFTNFMNYALLEKSDADVACTALFDSASGFKQVVT
HCCEEEEEEEEEEECCEEECCCCCCCHHHHHHHHEEECCCCCEEEEEEECCCCHHHHHHH
MRDVINNYPFPNTFKVLAVSGAKLKEAIERSAEYFDVKNDEVSVSADFLEPKPQHFNYDI
HHHHHHCCCCCCCEEEEEECCHHHHHHHHCCHHHEECCCCCEEEEECCCCCCCCCCCEEE
YGGVSYTIHVGRPKGQRVSNMMIQGHAVDLKQTYTICVNNYRAVGGGQYDMYIDAPVVKD
ECCEEEEEEECCCCCCHHHHEEEECEEECCCEEEEEEEECEEECCCCEEEEEECCCCEEC
IQVEGAQLLIDFLSNNNLMRIPQVVDFKVEK
EECCCHHHHHHHHCCCCEEECCCEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]