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The map label for this gene is pbp1 [H]

Identifier: 57650293

GI number: 57650293

Start: 1197640

End: 1199874

Strand: Direct

Name: pbp1 [H]

Synonym: SACOL1194

Alternate gene names: 57650293

Gene position: 1197640-1199874 (Clockwise)

Preceding gene: 57650292

Following gene: 57650294

Centisome position: 42.63

GC content: 34.45

Gene sequence:

>2235_bases
ATGGCGAAGCAAAAAATTAAAATTAAAAAAAATAAAATAGGGGCAGTCCTACTTGTTGGTTTATTCGGACTGCTCTTTTT
TATATTGGTTTTAAGAATTTCATATATCATGATTACTGGACATTCTAATGGTCAAGATTTAGTCATGAAGGCAAATGAAA
AGTATTTAGTTAAGAATGCACAACAACCAGAACGAGGAAAGATATATGATCGTAATGGTAAAGTGCTAGCAGAAGATGTA
GAAAGATATAAACTTGTTGCAGTAATAGATAAAAAGGCGAGTGCCAATTCTAAAAAACCTAGGCATGTAGTTGATAAAAA
AGAGACTGCAAAGAAATTATCTACAGTCATTAATATGAAGCCAGAGGAAATTGAAAAGAGACTTAGTCAAAAGAAAGCTT
TCCAAATTGAATTTGGACGCAAAGGAACAAATTTAACGTATCAGGACAAATTGAAAATAGAGAAAATGAATTTGCCTGGT
ATTTCTTTATTGCCTGAAACAGAACGCTTTTATCCAAATGGCAATTTTGCATCACACTTAATTGGTAGAGCTCAGAAAAA
TCCGGATACTGGTGAACTTAAAGGTGCACTTGGAGTTGAAAAGATTTTTGATAGTTATTTAAGTGGATCTAAAGGATCAT
TGAGATATATTCATGATATTTGGGGATATATCGCACCAAATACTAAAAAAGAGAAGCAGCCTAAACGTGGTGATGATGTC
CATTTAACAATCGATTCAAATATTCAAGTATTTGTTGAAGAAGCTTTAGATGGCATGGTTGAAAGATACCAGCCGAAAGA
TTTATTTGCGGTTGTCATGGATGCCAAAACTGGAGAAATTTTAGCATACAGTCAGCGACCAACATTTAATCCTGAAACTG
GTAAAGACTTTGGTAAAAAGTGGGCAAATGACCTTTATCAAAACACATACGAGCCTGGATCAACATTTAAATCATATGGG
TTAGCAGCTGCTATTCAAGAAGGTGCTTTTGATCCTGATAAGAAATATAAATCTGGACATAGAGATATTATGGGTTCACG
TATTTCAGACTGGAATAGAGTCGGTTGGGGTGAAATCCCAATGTCACTCGGATTTACTTATTCATCTAATACATTGATGA
TGCATTTACAAGATTTAGTTGGTGCAGACAAAATGAAATCTTGGTATGAACGATTTGGATTTGGAAAATCAACTAAAGGT
ATGTTTGATGGAGAAGCACCTGGTCAAATTGGATGGAGTAATGAGTTGCAACAAAAAACGTCATCATTTGGTCAATCGAC
AACAGTAACACCTGTTCAAATGTTACAAGCGCAATCAGCGTTCTTTAATGATGGTAATATGTTAAAACCATGGTTTGTGA
ATAGCGTTGAAAATCCTGTTAGTAAAAGACAATTTTATAAAGGGCAAAAACAAATCGCAGGCAAACCAATAACAAAAGAT
ACTGCTGAAAAAGTTGAAAAGCAATTGGATTTAGTTGTGAATAGTAAGAAGAGTCACGCTGCAAACTATCGTATTGATGG
TTATGAGGTCGAAGGTAAGACTGGTACAGCACAAGTCGCTGCACCTAATGGTGGTGGATACGTTAAAGGTCCAAACCCAT
ATTTTGTAAGTTTTATGGGTGACGCGCCGAAGAAAAATCCTAAAGTTATTGTATACGCTGGTATGAGCTTGGCACAAAAA
AATGACCAAGAAGCTTATGAATTAGGTGTTAGTAAAGCGTTTAAACCAATAATGGAAAATACTTTGAAATATTTAAATGT
AGGTAAATCAAAAGATGACACATCTAATGCAGAGTATAGTAAAGTGCCAGATGTTGAAGGTCAAGACAAACAAAAAGCTA
TTGATAATGTGAGTGCAAAATCATTAGAACCAGTTACTATTGGTTCTGGCACACAAATAAAAGCACAATCTATAAAAGCA
GGGAATAAAGTCTTACCTCATAGTAAAGTACTGTTATTAACAGATGGAGACTTAACTATGCCTGACATGTCAGGATGGAC
GAAAGAAGATGTCATTGCTTTTGAAAACCTAACAAATATTAAAGTAAATTTAAAAGGTAGCGGTTTTGTGTCCCACCAAT
CAATTAGTAAGGGACAAAAACTTACTGAAAAAGATAAAATAGACGTAGAATTTTCATCAGAGAATGTAGACAGCAATTCG
ACGAATAATTCTGATTCAAATTCAGATGATAAGAAGAAATCTGACAGTAAAACTGACAAGGATAAGTCGGACTAA

Upstream 100 bases:

>100_bases
TCTGAAATCAAAAAGAATTCTTCTTATGAACGCATATACGAAAAGGCTAAGAAACAGGGGATGAGCCTTGAGAACGATAA
TGTAAAGGTAGTGCGTAGTA

Downstream 100 bases:

>100_bases
CAAGAATGGCTACATTAAATTAGGATGTATTGTCACTATTTGTTTTTACTAAAGTAATGCTGTGATAGAAAACAACATCT
TCATTTAATCTTTATAAATA

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDV
ERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYG
LAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKG
MFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQK
NDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKA
GNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
TNNSDSNSDDKKKSDSKTDKDKSD

Sequences:

>Translated_744_residues
MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDV
ERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYG
LAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKG
MFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQK
NDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKA
GNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
TNNSDSNSDDKKKSDSKTDKDKSD
>Mature_743_residues
AKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVE
RYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGI
SLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDVH
LTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGL
AAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGM
FDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKDT
AEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKN
DQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAG
NKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNST
NNSDSNSDDKKKSDSKTDKDKSD

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=583, Percent_Identity=26.2435677530017, Blast_Score=152, Evalue=1e-37,
Organism=Escherichia coli, GI1786854, Length=551, Percent_Identity=24.5009074410163, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 82710; Mature: 82579

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNA
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCEECCC
QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMK
CCCCCCCEECCCCCEEHHHHHHEEEEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHCCC
PEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHL
HHHHHHHHHHCCEEEEEECCCCCCCEECCCEEEEEECCCCCEECCCHHHHCCCCCHHHHH
IGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK
EEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHH
WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIP
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCCC
MSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKT
CEEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
HHCCCCCCCCHHHHHHHHHHHCCCCCEECHHHHCCCCCHHHHHHHHCCHHHHCCCCCCHH
TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMG
HHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCCCEEEECCCCCCEEECCCCEEEEEEC
DAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYS
CCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTM
CCCCCCCCCHHHHHHCCCCCCCCCEEECCCCEEEHHHHCCCCEECCCCEEEEEECCCEEC
PDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
CCCCCCCHHHEEEECCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCEEEEECCCCCCCCC
TNNSDSNSDDKKKSDSKTDKDKSD
CCCCCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNA
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCEECCC
QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMK
CCCCCCCEECCCCCEEHHHHHHEEEEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHCCC
PEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHL
HHHHHHHHHHCCEEEEEECCCCCCCEECCCEEEEEECCCCCEECCCHHHHCCCCCHHHHH
IGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK
EEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHH
WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIP
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCCC
MSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKT
CEEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
HHCCCCCCCCHHHHHHHHHHHCCCCCEECHHHHCCCCCHHHHHHHHCCHHHHCCCCCCHH
TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMG
HHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCCCEEEECCCCCCEEECCCCEEEEEEC
DAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYS
CCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTM
CCCCCCCCCHHHHHHCCCCCCCCCEEECCCCEEEHHHHCCCCEECCCCEEEEEECCCEEC
PDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
CCCCCCCHHHEEEECCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCEEEEECCCCCCCCC
TNNSDSNSDDKKKSDSKTDKDKSD
CCCCCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]