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The map label for this gene is purB-2 [H]

Identifier: 57238433

GI number: 57238433

Start: 1482773

End: 1484140

Strand: Direct

Name: purB-2 [H]

Synonym: CJE1582

Alternate gene names: 57238433

Gene position: 1482773-1484140 (Clockwise)

Preceding gene: 57238432

Following gene: 57238434

Centisome position: 83.4

GC content: 35.53

Gene sequence:

>1368_bases
ATGACAGGAATTAGCGTATTTGATCATAGATTACTAGCAGACTCATGGAGCACGCAAGAAATGCGTGCTATTTTTTGCGA
ACAAAACCGCATACAAAAATGGCTCGATGTTGAAGCAGCTTTAGCAAAAGCACAAGCAAAACTTAAAATTATCCCTAAAA
AAGCTGCCGATGAAATTGCTAAAAAAGCACATTATAAATTTATGGATATGGATTTCATTTTCGCAGAATATAAAAAGACT
AAGCACCCCCTAGTGCCAACGGTAAGAGGATTAGAAAAAGCTTGCGATGATAATTTGGGTGAATATGTGCATTTTGGAGT
AACCACTCAAGATGTCATTGATACGGGGCTTGTATTGCAGTTTAAAGAAGCGATGATTTTGGTAAAAAGTGAATTAAAAG
CTATCGCAAAAGCCTTGGCGAAACTAGCTAAAACCCATAAAAATACTGCTATGATGGGAAGAACTTTAGCGCTTCAGGCT
TTGCCTATTACTTTTGGACATAAGGTTGCCATTTGGCTTAGTGAGCTTGATCGCCATTTTGAAAGAATACTTGAGCTTGA
AAAAAGACTTTATGTAGGCAGTATAGTAGGTGCTGTAGGAACTAAAGCAAGTTTAAGCGATGAGTGCAATGAGGTAGAAA
AACTCACTTTAGAAAATTTAGGACTTGATGTGCCTAATATCTCTTGGCAGCCAGCAAGAGACCGCTTTATAGAACTTGGT
TTTGTTTTGGGTAATATTAATGCCACTTTTAACAAAATCGCTCATGAAATTTTAATCCTTTCGCATAATGAAATTGATGA
AGTTGCTGAACCTTTTGGCAAAGGACAAGTAGGATCAAGCACTATGCCACACAAAAGAAATCCTGCAGTAAGCGAAAATG
CAGTTACAGTAAGCAATGCCTTTAAAGCAAATTTAGCCATTTTAAGTGATATTGAAAGACATGAACATGAAAGAGATGGT
CAAGTTTGGAAAATGGAATGGAAGCTTTTACCTGAAATGTTTTTAATGCTTTCTGTGGTTTTAGCAAATATGAAATTCGC
TCTTAGTGATTTAGAAGTAAAAAAAGATAAAATGCTTAAAAATCTCAACACCCTTAATGGCTTTGTTTTAGCCGAACGCG
TGATGTTTGCTTTGAGCGATCATTATGGTAAGCAGCACGCTCACGAAATCGTTTATGAAAATGCTATGCTAGGTATAGAA
AAACAAAAAACTTTTAAAGAAGTTTTACTTGCAGATAAAAGAGTATCTAAGGTATTAAAAGAAAAAGAAATCGATGCCTT
GCTTGATGCTAGCACTTATGTAGGATATGCACCAAAACTTGTCGATGAATTTTTGGCAAAAATCAAAAATAGTGCTATCT
TAAAATAG

Upstream 100 bases:

>100_bases
AGGGCCATTTGTAAGATTTCATATAGGTCTTGAAGATACTAAAGATTTAATTGCTGATTTAACACAAGCTTTTGATAGTA
TTAAAAAATAAGGAGATAAG

Downstream 100 bases:

>100_bases
GAAAAATCATGTTCTATAGTGAAATTTTAGCGGATTTTATTTTCAATCTTAAATATGAAAATATCCCTGATGCAGTTGTT
CAAAGGGCTAAAGAGCTTAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTGISVFDHRLLADSWSTQEMRAIFCEQNRIQKWLDVEAALAKAQAKLKIIPKKAADEIAKKAHYKFMDMDFIFAEYKKT
KHPLVPTVRGLEKACDDNLGEYVHFGVTTQDVIDTGLVLQFKEAMILVKSELKAIAKALAKLAKTHKNTAMMGRTLALQA
LPITFGHKVAIWLSELDRHFERILELEKRLYVGSIVGAVGTKASLSDECNEVEKLTLENLGLDVPNISWQPARDRFIELG
FVLGNINATFNKIAHEILILSHNEIDEVAEPFGKGQVGSSTMPHKRNPAVSENAVTVSNAFKANLAILSDIERHEHERDG
QVWKMEWKLLPEMFLMLSVVLANMKFALSDLEVKKDKMLKNLNTLNGFVLAERVMFALSDHYGKQHAHEIVYENAMLGIE
KQKTFKEVLLADKRVSKVLKEKEIDALLDASTYVGYAPKLVDEFLAKIKNSAILK

Sequences:

>Translated_455_residues
MTGISVFDHRLLADSWSTQEMRAIFCEQNRIQKWLDVEAALAKAQAKLKIIPKKAADEIAKKAHYKFMDMDFIFAEYKKT
KHPLVPTVRGLEKACDDNLGEYVHFGVTTQDVIDTGLVLQFKEAMILVKSELKAIAKALAKLAKTHKNTAMMGRTLALQA
LPITFGHKVAIWLSELDRHFERILELEKRLYVGSIVGAVGTKASLSDECNEVEKLTLENLGLDVPNISWQPARDRFIELG
FVLGNINATFNKIAHEILILSHNEIDEVAEPFGKGQVGSSTMPHKRNPAVSENAVTVSNAFKANLAILSDIERHEHERDG
QVWKMEWKLLPEMFLMLSVVLANMKFALSDLEVKKDKMLKNLNTLNGFVLAERVMFALSDHYGKQHAHEIVYENAMLGIE
KQKTFKEVLLADKRVSKVLKEKEIDALLDASTYVGYAPKLVDEFLAKIKNSAILK
>Mature_454_residues
TGISVFDHRLLADSWSTQEMRAIFCEQNRIQKWLDVEAALAKAQAKLKIIPKKAADEIAKKAHYKFMDMDFIFAEYKKTK
HPLVPTVRGLEKACDDNLGEYVHFGVTTQDVIDTGLVLQFKEAMILVKSELKAIAKALAKLAKTHKNTAMMGRTLALQAL
PITFGHKVAIWLSELDRHFERILELEKRLYVGSIVGAVGTKASLSDECNEVEKLTLENLGLDVPNISWQPARDRFIELGF
VLGNINATFNKIAHEILILSHNEIDEVAEPFGKGQVGSSTMPHKRNPAVSENAVTVSNAFKANLAILSDIERHEHERDGQ
VWKMEWKLLPEMFLMLSVVLANMKFALSDLEVKKDKMLKNLNTLNGFVLAERVMFALSDHYGKQHAHEIVYENAMLGIEK
QKTFKEVLLADKRVSKVLKEKEIDALLDASTYVGYAPKLVDEFLAKIKNSAILK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=453, Percent_Identity=23.841059602649, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI183227688, Length=299, Percent_Identity=25.752508361204, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1787376, Length=479, Percent_Identity=25.4697286012526, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508577, Length=449, Percent_Identity=26.0579064587973, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=359, Percent_Identity=27.2980501392758, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=466, Percent_Identity=24.8927038626609, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24647570, Length=301, Percent_Identity=25.2491694352159, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51281; Mature: 51150

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGISVFDHRLLADSWSTQEMRAIFCEQNRIQKWLDVEAALAKAQAKLKIIPKKAADEIA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHH
KKAHYKFMDMDFIFAEYKKTKHPLVPTVRGLEKACDDNLGEYVHFGVTTQDVIDTGLVLQ
HHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHH
FKEAMILVKSELKAIAKALAKLAKTHKNTAMMGRTLALQALPITFGHKVAIWLSELDRHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEECCCHHHHHHHHHHHHH
ERILELEKRLYVGSIVGAVGTKASLSDECNEVEKLTLENLGLDVPNISWQPARDRFIELG
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FVLGNINATFNKIAHEILILSHNEIDEVAEPFGKGQVGSSTMPHKRNPAVSENAVTVSNA
HHEECCCHHHHHHHHHHEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
FKANLAILSDIERHEHERDGQVWKMEWKLLPEMFLMLSVVLANMKFALSDLEVKKDKMLK
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLNTLNGFVLAERVMFALSDHYGKQHAHEIVYENAMLGIEKQKTFKEVLLADKRVSKVLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EKEIDALLDASTYVGYAPKLVDEFLAKIKNSAILK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TGISVFDHRLLADSWSTQEMRAIFCEQNRIQKWLDVEAALAKAQAKLKIIPKKAADEIA
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHH
KKAHYKFMDMDFIFAEYKKTKHPLVPTVRGLEKACDDNLGEYVHFGVTTQDVIDTGLVLQ
HHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHH
FKEAMILVKSELKAIAKALAKLAKTHKNTAMMGRTLALQALPITFGHKVAIWLSELDRHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEECCCHHHHHHHHHHHHH
ERILELEKRLYVGSIVGAVGTKASLSDECNEVEKLTLENLGLDVPNISWQPARDRFIELG
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FVLGNINATFNKIAHEILILSHNEIDEVAEPFGKGQVGSSTMPHKRNPAVSENAVTVSNA
HHEECCCHHHHHHHHHHEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
FKANLAILSDIERHEHERDGQVWKMEWKLLPEMFLMLSVVLANMKFALSDLEVKKDKMLK
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLNTLNGFVLAERVMFALSDHYGKQHAHEIVYENAMLGIEKQKTFKEVLLADKRVSKVLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EKEIDALLDASTYVGYAPKLVDEFLAKIKNSAILK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]