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The map label for this gene is metC [H]

Identifier: 57238432

GI number: 57238432

Start: 1481600

End: 1482763

Strand: Direct

Name: metC [H]

Synonym: CJE1581

Alternate gene names: 57238432

Gene position: 1481600-1482763 (Clockwise)

Preceding gene: 57238431

Following gene: 57238433

Centisome position: 83.34

GC content: 35.22

Gene sequence:

>1164_bases
ATGAACTCAAAAACTAAACTTATTCATTGTGGCAGAGGCGATCAAGGCGCTGAAGTAAGATCAGTCAATCCTACACTTAT
GCGTGCATCAACCATACTTTTTAAAGATCACGCAACTTGGCAAAAATACAGAGGATTAAGAAAAACAGATCGTGTTTTAA
GTTATGGAGCTAGAGGAACAACAACCAATTTTGAACTTGAAAAACTTATTTGCACTCTAGAGGGTGGTTATAGAGCACAA
CTTTTCCCAACAGGACTTGCAGCATTAGCTATGGTGCTTTTAAACTATGCAAGCAAAGATGCACATTTTTTAATCACTGA
TGCAATTTATGGGCCTGTAAGAACGATTTGCGAATTATTTTTAGACAAAATGGGAGTTGAAATTGATTTTTTAAAAGCTG
ATGCAAGTGATGTAGAAGAAAAAATCAAACCTAATACCAAGCTTATCCTATGTGAAAGTCCAGGTTCTATACTTTATGAA
ATCATAGATCTACCAAAGCTTTGTAAAATCGCCCACTCTTACAATATCCCTGTAGCGATTGACAATACTTATTCTAGTGG
ATATTTTCTAAATCCTTTAGAACTTGGAGTGGATATTTCAGTTATCGCCGCTACAAAATATCTTAGCGGACACTCTGATG
TAACCATGGGTATAGTAGTAATCAACGAAAAAGAGTGGAAAAATTTTGACAAACTTCCAGAAGCTTTAGGCTTTACAACA
AGTCCTGATGATGCTTACTTAGTGCTTCGTGGCATGAGAACCTTAGATGTGAGAATGAAAGCTCACGAAAAAAGTGCTGA
TGAAATTGTAGAGTTTTTACAAAGCAGAAAAGAAATTAAAACAATTTTCTATCCAAAACTCAAATCCCACCCAAATCATG
AAATTTTCATGCGTGATCATAAAGGTGCAAATGGCATGATTACCATAGAATTTGCAGAAGATTACACTAAAGATGATGCG
ATTAAATTTGTAGATTTATTGCAATTTTTCTCCATAGGTGCTAGTTGGGGTGGCTATGAAAGTTTAGCCACAGTAACAAC
TCCGCCAAGAACTGCAACAGACCTAAGTGCAAGAGGGCCATTTGTAAGATTTCATATAGGTCTTGAAGATACTAAAGATT
TAATTGCTGATTTAACACAAGCTTTTGATAGTATTAAAAAATAA

Upstream 100 bases:

>100_bases
AAGTCCTTTTGCCGTGGTTAAAAGAACAGCTATTCCTATGCTTGTAGCGGCTATTGTAAATGTTATTATGACTTATATCT
ATCTTTAAAAAGGAGAAAAA

Downstream 100 bases:

>100_bases
GGAGATAAGATGACAGGAATTAGCGTATTTGATCATAGATTACTAGCAGACTCATGGAGCACGCAAGAAATGCGTGCTAT
TTTTTGCGAACAAAACCGCA

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase; Osteotoxin [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNSKTKLIHCGRGDQGAEVRSVNPTLMRASTILFKDHATWQKYRGLRKTDRVLSYGARGTTTNFELEKLICTLEGGYRAQ
LFPTGLAALAMVLLNYASKDAHFLITDAIYGPVRTICELFLDKMGVEIDFLKADASDVEEKIKPNTKLILCESPGSILYE
IIDLPKLCKIAHSYNIPVAIDNTYSSGYFLNPLELGVDISVIAATKYLSGHSDVTMGIVVINEKEWKNFDKLPEALGFTT
SPDDAYLVLRGMRTLDVRMKAHEKSADEIVEFLQSRKEIKTIFYPKLKSHPNHEIFMRDHKGANGMITIEFAEDYTKDDA
IKFVDLLQFFSIGASWGGYESLATVTTPPRTATDLSARGPFVRFHIGLEDTKDLIADLTQAFDSIKK

Sequences:

>Translated_387_residues
MNSKTKLIHCGRGDQGAEVRSVNPTLMRASTILFKDHATWQKYRGLRKTDRVLSYGARGTTTNFELEKLICTLEGGYRAQ
LFPTGLAALAMVLLNYASKDAHFLITDAIYGPVRTICELFLDKMGVEIDFLKADASDVEEKIKPNTKLILCESPGSILYE
IIDLPKLCKIAHSYNIPVAIDNTYSSGYFLNPLELGVDISVIAATKYLSGHSDVTMGIVVINEKEWKNFDKLPEALGFTT
SPDDAYLVLRGMRTLDVRMKAHEKSADEIVEFLQSRKEIKTIFYPKLKSHPNHEIFMRDHKGANGMITIEFAEDYTKDDA
IKFVDLLQFFSIGASWGGYESLATVTTPPRTATDLSARGPFVRFHIGLEDTKDLIADLTQAFDSIKK
>Mature_387_residues
MNSKTKLIHCGRGDQGAEVRSVNPTLMRASTILFKDHATWQKYRGLRKTDRVLSYGARGTTTNFELEKLICTLEGGYRAQ
LFPTGLAALAMVLLNYASKDAHFLITDAIYGPVRTICELFLDKMGVEIDFLKADASDVEEKIKPNTKLILCESPGSILYE
IIDLPKLCKIAHSYNIPVAIDNTYSSGYFLNPLELGVDISVIAATKYLSGHSDVTMGIVVINEKEWKNFDKLPEALGFTT
SPDDAYLVLRGMRTLDVRMKAHEKSADEIVEFLQSRKEIKTIFYPKLKSHPNHEIFMRDHKGANGMITIEFAEDYTKDDA
IKFVDLLQFFSIGASWGGYESLATVTTPPRTATDLSARGPFVRFHIGLEDTKDLIADLTQAFDSIKK

Specific function: Also has cytotoxic activity toward osteogenic, osteosarcoma and tracheal cells, in vitro. The chemical basis for cell toxicity might be the formation and subsequent transfer of sulfane-sulfur to proteins, derived via beta-cystathionase cleavage of L-cysti

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=344, Percent_Identity=35.1744186046512, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI299473759, Length=227, Percent_Identity=40.0881057268723, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI25453487, Length=343, Percent_Identity=31.1953352769679, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=34.9489795918367, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1790375, Length=352, Percent_Identity=30.9659090909091, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=34.1025641025641, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=394, Percent_Identity=34.5177664974619, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=340, Percent_Identity=33.5294117647059, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=318, Percent_Identity=35.2201257861635, Blast_Score=215, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=342, Percent_Identity=33.9181286549708, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=26.6497461928934, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=26.3761467889908, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321254, Length=331, Percent_Identity=25.3776435045317, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922554, Length=394, Percent_Identity=31.9796954314721, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006233
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 43311; Mature: 43311

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKTKLIHCGRGDQGAEVRSVNPTLMRASTILFKDHATWQKYRGLRKTDRVLSYGARGT
CCCCCCEEECCCCCCCCEEECCCHHHHHHEEEEEECCCCHHHHCCCHHHHHHHHCCCCCC
TTNFELEKLICTLEGGYRAQLFPTGLAALAMVLLNYASKDAHFLITDAIYGPVRTICELF
CCCCEEEEEEEEECCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHH
LDKMGVEIDFLKADASDVEEKIKPNTKLILCESPGSILYEIIDLPKLCKIAHSYNIPVAI
HHHHCCEEEEEECCHHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHCCCCCEEE
DNTYSSGYFLNPLELGVDISVIAATKYLSGHSDVTMGIVVINEKEWKNFDKLPEALGFTT
ECCCCCCEEECHHHHCCCEEEEEEEHHHCCCCCEEEEEEEEECHHHCCHHHHHHHHCCCC
SPDDAYLVLRGMRTLDVRMKAHEKSADEIVEFLQSRKEIKTIFYPKLKSHPNHEIFMRDH
CCCCCEEEEECCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEC
KGANGMITIEFAEDYTKDDAIKFVDLLQFFSIGASWGGYESLATVTTPPRTATDLSARGP
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCC
FVRFHIGLEDTKDLIADLTQAFDSIKK
EEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSKTKLIHCGRGDQGAEVRSVNPTLMRASTILFKDHATWQKYRGLRKTDRVLSYGARGT
CCCCCCEEECCCCCCCCEEECCCHHHHHHEEEEEECCCCHHHHCCCHHHHHHHHCCCCCC
TTNFELEKLICTLEGGYRAQLFPTGLAALAMVLLNYASKDAHFLITDAIYGPVRTICELF
CCCCEEEEEEEEECCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHH
LDKMGVEIDFLKADASDVEEKIKPNTKLILCESPGSILYEIIDLPKLCKIAHSYNIPVAI
HHHHCCEEEEEECCHHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHCCCCCEEE
DNTYSSGYFLNPLELGVDISVIAATKYLSGHSDVTMGIVVINEKEWKNFDKLPEALGFTT
ECCCCCCEEECHHHHCCCEEEEEEEHHHCCCCCEEEEEEEEECHHHCCHHHHHHHHCCCC
SPDDAYLVLRGMRTLDVRMKAHEKSADEIVEFLQSRKEIKTIFYPKLKSHPNHEIFMRDH
CCCCCEEEEECCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEC
KGANGMITIEFAEDYTKDDAIKFVDLLQFFSIGASWGGYESLATVTTPPRTATDLSARGP
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCC
FVRFHIGLEDTKDLIADLTQAFDSIKK
EEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8463265 [H]