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The map label for this gene is ribF [H]

Identifier: 57238215

GI number: 57238215

Start: 625326

End: 626180

Strand: Direct

Name: ribF [H]

Synonym: CJE0692

Alternate gene names: 57238215

Gene position: 625326-626180 (Clockwise)

Preceding gene: 57238216

Following gene: 57238214

Centisome position: 35.17

GC content: 27.02

Gene sequence:

>855_bases
ATGTTGAATATTTTTACTACTATATCAAAAATTAATATTACTTCTTTAGCCATAGGTTGTTTTGATGGTATGCATTTAGG
ACATCTAAAACTTGTAAAATATTTAGATGAGAATGGGGCTTTGCTAGTTATTAATAAATTTAAAGGTCAATTTCTTTGCT
CAAATCGTCAAAAAGAAGAAATTTCAGGAAAAAAAGTTATAGAAGTGGATTTTGAAAGTATAAAATCTTTAGATGGTAAA
GATTTTTTAAGCTTTTTGAAAAAAGAATTTACCAGTTTAAAATTTATAGTCGTGGGTTATGATTTTTCTTTTGGGAAAAA
TAGGGCTTATAGTGCTAAAGATATTGAGTTTTTAAGTGGGATAAAAACTATAATAGTTGATGAATTTAGCATAGGTGGAA
TAGGTGTTCATGCAAGTTTAATTAAGGATTTTTTATCAAGAGCAAATTTGCAAAAAGTAAAAGAATTTTTAGGGAGAAAT
TATAGCATCAAAGGAAAGGTAATTAAAGGACAAGGCTTAGGTTCTAAAGAACTTTTTGCAACTATAAATTTAGATTGCGA
AGGTTATTTTTTGCCTCAAAATGGTGTTTACGCTACTTTGTTAAAAAGTCAAAGAAAAACTTATAAAAGTGTGAGTTTTT
TAGGTGTAAGATCAAGCGATGAAAATTTTGCTATAGAAAGTCATATTTTAGAAGAATTGGGTGAAAATTTCACGCAAGGA
GAGTTTTTTGAACTCGAGTTTATATCTTTTCTAAGAGAGAATCAAAAATTTCAAGATCTAAAAAAACTTAAAAATCAAAT
TACTAAAGATATAGAACAGGCTAGAGAATTATTAAGGAAAAATGATGAAAGATGA

Upstream 100 bases:

>100_bases
GCAATCTTAAAAGCAAGAATGGATTTTGAAAAAGAATGTGCTAAGTTAGGTTGGCTTTTAAAAAATACGCAAAAATCAAG
CATTAAAGGAAAGGAAGGTA

Downstream 100 bases:

>100_bases
ACTTTTTAAACAAAGCCCTAAAAAACAATTTGAATTTGATAAAAGCGTGGCTAGTGTTTTTGATGATATGATCAATCGTT
CTGTACCATTTTATAGAGAA

Product: bifunctional riboflavin kinase/FMN adenylyltransferase

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLNIFTTISKINITSLAIGCFDGMHLGHLKLVKYLDENGALLVINKFKGQFLCSNRQKEEISGKKVIEVDFESIKSLDGK
DFLSFLKKEFTSLKFIVVGYDFSFGKNRAYSAKDIEFLSGIKTIIVDEFSIGGIGVHASLIKDFLSRANLQKVKEFLGRN
YSIKGKVIKGQGLGSKELFATINLDCEGYFLPQNGVYATLLKSQRKTYKSVSFLGVRSSDENFAIESHILEELGENFTQG
EFFELEFISFLRENQKFQDLKKLKNQITKDIEQARELLRKNDER

Sequences:

>Translated_284_residues
MLNIFTTISKINITSLAIGCFDGMHLGHLKLVKYLDENGALLVINKFKGQFLCSNRQKEEISGKKVIEVDFESIKSLDGK
DFLSFLKKEFTSLKFIVVGYDFSFGKNRAYSAKDIEFLSGIKTIIVDEFSIGGIGVHASLIKDFLSRANLQKVKEFLGRN
YSIKGKVIKGQGLGSKELFATINLDCEGYFLPQNGVYATLLKSQRKTYKSVSFLGVRSSDENFAIESHILEELGENFTQG
EFFELEFISFLRENQKFQDLKKLKNQITKDIEQARELLRKNDER
>Mature_284_residues
MLNIFTTISKINITSLAIGCFDGMHLGHLKLVKYLDENGALLVINKFKGQFLCSNRQKEEISGKKVIEVDFESIKSLDGK
DFLSFLKKEFTSLKFIVVGYDFSFGKNRAYSAKDIEFLSGIKTIIVDEFSIGGIGVHASLIKDFLSRANLQKVKEFLGRN
YSIKGKVIKGQGLGSKELFATINLDCEGYFLPQNGVYATLLKSQRKTYKSVSFLGVRSSDENFAIESHILEELGENFTQG
EFFELEFISFLRENQKFQDLKKLKNQITKDIEQARELLRKNDER

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=290, Percent_Identity=26.8965517241379, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 32312; Mature: 32312

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIFTTISKINITSLAIGCFDGMHLGHLKLVKYLDENGALLVINKFKGQFLCSNRQKEE
CCCCEEHHHHCCHHHEEEEHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCHHH
ISGKKVIEVDFESIKSLDGKDFLSFLKKEFTSLKFIVVGYDFSFGKNRAYSAKDIEFLSG
CCCCEEEEEEHHHHHCCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCHHHHHHHHC
IKTIIVDEFSIGGIGVHASLIKDFLSRANLQKVKEFLGRNYSIKGKVIKGQGLGSKELFA
CCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCEEE
TINLDCEGYFLPQNGVYATLLKSQRKTYKSVSFLGVRSSDENFAIESHILEELGENFTQG
EEEECCCEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCC
EFFELEFISFLRENQKFQDLKKLKNQITKDIEQARELLRKNDER
CEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNIFTTISKINITSLAIGCFDGMHLGHLKLVKYLDENGALLVINKFKGQFLCSNRQKEE
CCCCEEHHHHCCHHHEEEEHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCHHH
ISGKKVIEVDFESIKSLDGKDFLSFLKKEFTSLKFIVVGYDFSFGKNRAYSAKDIEFLSG
CCCCEEEEEEHHHHHCCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCHHHHHHHHC
IKTIIVDEFSIGGIGVHASLIKDFLSRANLQKVKEFLGRNYSIKGKVIKGQGLGSKELFA
CCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCEEE
TINLDCEGYFLPQNGVYATLLKSQRKTYKSVSFLGVRSSDENFAIESHILEELGENFTQG
EEEECCCEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCC
EFFELEFISFLRENQKFQDLKKLKNQITKDIEQARELLRKNDER
CEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7772835; 3023344 [H]