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The map label for this gene is hypE [H]

Identifier: 57238179

GI number: 57238179

Start: 662531

End: 663505

Strand: Direct

Name: hypE [H]

Synonym: CJE0729

Alternate gene names: 57238179

Gene position: 662531-663505 (Clockwise)

Preceding gene: 57238180

Following gene: 57238178

Centisome position: 37.27

GC content: 33.85

Gene sequence:

>975_bases
ATGAAAAATATTTCCTTAGCCCACGGAGGCGGTGGAGAAGAGATGAATGAGCTTTTAACTAAGCTTTTTAAGATCTTTGA
TAATGAAATTTTAAACGCAAATAATGATGCAGCGATTTTGGGAAATTTGGCTTTAAGCACGGATTCTTTTGTTTTAAGTC
CGATTTTTTTAGATGAAGAGGTTAATATAGGTAAACTTTGTGTTTGTGGTTCTATTAATGATGTTTTGATGGTGGGTGCT
AAGCCAAAATATCTAAGCTTAGGGCTTATCTTAGAAGAAGGTTTTGAGCTAGAAAAATTAGAACGTATTTTAAAAAGCAT
TAAAGAAGAATGTGAAAAATGTGGAGTTATGTTAGTTTGTGGCGATACTAAAGTTGTTCCTAAGGGTAAAGCAGATGAAA
TTTATATCAACACTACAGCCTTAGGTGAAATTATTTCTAAAAAAGAAAGTAAAAATATCAAAGCAGGGCTTAGCATACTT
CTTTCAGGTGATATAGGAAGGCATGGAGCTAGTGTTTTAATCAAAAGAAATGAGTTAGAAGCTAATGTTAAAAGTGATTG
TAAGGCTTTAGATAAAGAGGTTTTAGAGCTTTTAGAAAAAGATATAAAGGTTGTAGCAATGAGAGATGCTACTCGTGGGG
GACTTAGTGCGGTTTTAAATGAGTGGGCAAAGCAAAGTGGAAATGATCTTTTGATCTTTGAAGAAAAAATCATCGTTCAA
GATGAGGTTTTAGGACTTTGCGAGCTTTTTGGTTATGAAGCCTATGAGCTTGCAAATGAAGGCACTTTTATACTTTGTGT
GGAAAAAGAAGATGGGTTAAAAGCCTTGGAAATTCTAAAAAAATACAATGTAAATGCGAGTATTATAGGAGAAGTTTTAG
AAGAGAAAAAAGCACGCGTGATCTTACAAAACGCTTATGGAGCAAAACGCTTTTTAGAAAGCCCTAAAGGCGAACTTTTA
CCAAGAATTTGCTAA

Upstream 100 bases:

>100_bases
TTTTTGGCAAGGCTTGTACGCCAAGAAGTCCTATAGGAAGTTGTATGGTTTCTGGCGAGGGTGCTTGTGCGGCGTATTAT
AAATACTCAAAGGTGAATGC

Downstream 100 bases:

>100_bases
TGCACGAATTAAGTATAGTAGAATCTTTAATCGAACTTTGTGAAGAAAATGCCTTAAACAATAAGGCTTACAATGTGCAA
GAAATTTATGTAAAAATCGG

Product: hydrogenase expression/formation protein HypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKNISLAHGGGGEEMNELLTKLFKIFDNEILNANNDAAILGNLALSTDSFVLSPIFLDEEVNIGKLCVCGSINDVLMVGA
KPKYLSLGLILEEGFELEKLERILKSIKEECEKCGVMLVCGDTKVVPKGKADEIYINTTALGEIISKKESKNIKAGLSIL
LSGDIGRHGASVLIKRNELEANVKSDCKALDKEVLELLEKDIKVVAMRDATRGGLSAVLNEWAKQSGNDLLIFEEKIIVQ
DEVLGLCELFGYEAYELANEGTFILCVEKEDGLKALEILKKYNVNASIIGEVLEEKKARVILQNAYGAKRFLESPKGELL
PRIC

Sequences:

>Translated_324_residues
MKNISLAHGGGGEEMNELLTKLFKIFDNEILNANNDAAILGNLALSTDSFVLSPIFLDEEVNIGKLCVCGSINDVLMVGA
KPKYLSLGLILEEGFELEKLERILKSIKEECEKCGVMLVCGDTKVVPKGKADEIYINTTALGEIISKKESKNIKAGLSIL
LSGDIGRHGASVLIKRNELEANVKSDCKALDKEVLELLEKDIKVVAMRDATRGGLSAVLNEWAKQSGNDLLIFEEKIIVQ
DEVLGLCELFGYEAYELANEGTFILCVEKEDGLKALEILKKYNVNASIIGEVLEEKKARVILQNAYGAKRFLESPKGELL
PRIC
>Mature_324_residues
MKNISLAHGGGGEEMNELLTKLFKIFDNEILNANNDAAILGNLALSTDSFVLSPIFLDEEVNIGKLCVCGSINDVLMVGA
KPKYLSLGLILEEGFELEKLERILKSIKEECEKCGVMLVCGDTKVVPKGKADEIYINTTALGEIISKKESKNIKAGLSIL
LSGDIGRHGASVLIKRNELEANVKSDCKALDKEVLELLEKDIKVVAMRDATRGGLSAVLNEWAKQSGNDLLIFEEKIIVQ
DEVLGLCELFGYEAYELANEGTFILCVEKEDGLKALEILKKYNVNASIIGEVLEEKKARVILQNAYGAKRFLESPKGELL
PRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=341, Percent_Identity=41.0557184750733, Blast_Score=237, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 35506; Mature: 35506

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNISLAHGGGGEEMNELLTKLFKIFDNEILNANNDAAILGNLALSTDSFVLSPIFLDEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEECCC
VNIGKLCVCGSINDVLMVGAKPKYLSLGLILEEGFELEKLERILKSIKEECEKCGVMLVC
CCCCCEEEECCCCCEEEECCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEE
GDTKVVPKGKADEIYINTTALGEIISKKESKNIKAGLSILLSGDIGRHGASVLIKRNELE
CCEEECCCCCCCEEEEEHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCEEEEECCCCC
ANVKSDCKALDKEVLELLEKDIKVVAMRDATRGGLSAVLNEWAKQSGNDLLIFEEKIIVQ
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEH
DEVLGLCELFGYEAYELANEGTFILCVEKEDGLKALEILKKYNVNASIIGEVLEEKKARV
HHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHE
ILQNAYGAKRFLESPKGELLPRIC
EECCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKNISLAHGGGGEEMNELLTKLFKIFDNEILNANNDAAILGNLALSTDSFVLSPIFLDEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEECCC
VNIGKLCVCGSINDVLMVGAKPKYLSLGLILEEGFELEKLERILKSIKEECEKCGVMLVC
CCCCCEEEECCCCCEEEECCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEE
GDTKVVPKGKADEIYINTTALGEIISKKESKNIKAGLSILLSGDIGRHGASVLIKRNELE
CCEEECCCCCCCEEEEEHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCEEEEECCCCC
ANVKSDCKALDKEVLELLEKDIKVVAMRDATRGGLSAVLNEWAKQSGNDLLIFEEKIIVQ
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEH
DEVLGLCELFGYEAYELANEGTFILCVEKEDGLKALEILKKYNVNASIIGEVLEEKKARV
HHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHE
ILQNAYGAKRFLESPKGELLPRIC
EECCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1849603; 9278503 [H]