Click here to switch to the map view.

The map label for this gene is intA [H]

Identifier: 57238154

GI number: 57238154

Start: 1335926

End: 1337101

Strand: Direct

Name: intA [H]

Synonym: CJE1418

Alternate gene names: 57238154

Gene position: 1335926-1337101 (Clockwise)

Preceding gene: 57238153

Following gene: 57238174

Centisome position: 75.14

GC content: 29.08

Gene sequence:

>1176_bases
ATGGCAAAAATTAACAAACTTACCGATAGTTTTTTAAAAAGCATTAAATGCGAAAGTGATAAAAAATTTATAAAATTTGC
TGATCCGAGTTTAAAAGGCTTATATGTTTTTATTTATCCTAGTGGTAAAAAATTATTTAAAATAAGACAAGCAAATGATA
CTTATATAAAAATAGGAGAGTATCCGTTACTTTCCTTAGCAGAGCTTAGAGAAATCGCTTTAAATGCTTTTAAGCTAAAA
GCAAAAGGACAAAACATAAAAAACGCTAAAAGACTTAAATTTGGCGATATATATGATGAAGTTTTAGAAAAATGTAAGGC
TGATGGATTAAGTCTAAAAGAAATACAAAGAGGATTAAAATACAAAAATGGCGTTTTTAAGAATTTCAAAGATGTAGATA
TAGAAAGCATCAAGCGTTCACATGTCATCGAAACACTTAAAACCATAGAGCATCAAATCCCTACACTTATAAAAGCTAAA
GGCTGGATTAACAAAATCTTTAAATATGCTTTGCAACTTGAAATCGTAGAAAATAACCCTGTGACAAGTATTGATAATTC
CATAGTTTTTAAAAAAGCTTCAAAAGTAATTCATCAGCCAACTCTACTAGAAAACGATAAAATTAAAGAATATATATTAG
CCTTAAAAAATTCAGATCTTAAAAAAACACATAAAAATCTAATGCTTTTTAATCTTTTGACTGCACAACGCCCCGGTAAT
GTTATAAAAGCAACTTGGGATGAAATTGATTTAAAAAATGCTATATGGACTATTAAAGCTGAAAAAATGAAAATGAGAAA
AGAACATATTATCACTTTAAATTCTCAAGCGCTTAAAATTTTAAAAGAACAAAAGAAAATGAAAGTCAATGATTATATAT
TTGCTGGAAGTTCAAAAAATGGGTGTAATAGCGAGAATATCACCTGTAATATCAATAAACGACTAGGATATAAAGGTATT
CAAAGTGCACATGGCTTTCGTGCTATGTTTAGAAGTTTAGCCAACGAACATCAATTAGATCATGGTGTGAGTATGGATAT
AGCCGAGCAGTGTTTAGCACACGAGCAAAAAAACGCCATTTTAAAAGCTTATAATCGTAGTGAAAATATAGAATTAAAAA
GAAAGTTAATGCAGTGGTGGGGAGATTATATCGAAAAACTTGCAGGGGATTTTTAA

Upstream 100 bases:

>100_bases
CTATAATGTTCTTAAAAAAGTGGGTCACTTTTCCAAAAATGGGTTACAAAATGGGCTACTTTTTACACCAAAATAGACAC
TAAAAAAGAGAGAAAAATTA

Downstream 100 bases:

>100_bases
ATCACCTTGCAAGTTTGCAACCATTTATCAATTTCGGCTTTTTCATATTTAAAAGTAGAACCTATTTTAATCCCTTTAGG
AAAATTAAATTTATTTTGAT

Product: phage integrase family site specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKINKLTDSFLKSIKCESDKKFIKFADPSLKGLYVFIYPSGKKLFKIRQANDTYIKIGEYPLLSLAELREIALNAFKLK
AKGQNIKNAKRLKFGDIYDEVLEKCKADGLSLKEIQRGLKYKNGVFKNFKDVDIESIKRSHVIETLKTIEHQIPTLIKAK
GWINKIFKYALQLEIVENNPVTSIDNSIVFKKASKVIHQPTLLENDKIKEYILALKNSDLKKTHKNLMLFNLLTAQRPGN
VIKATWDEIDLKNAIWTIKAEKMKMRKEHIITLNSQALKILKEQKKMKVNDYIFAGSSKNGCNSENITCNINKRLGYKGI
QSAHGFRAMFRSLANEHQLDHGVSMDIAEQCLAHEQKNAILKAYNRSENIELKRKLMQWWGDYIEKLAGDF

Sequences:

>Translated_391_residues
MAKINKLTDSFLKSIKCESDKKFIKFADPSLKGLYVFIYPSGKKLFKIRQANDTYIKIGEYPLLSLAELREIALNAFKLK
AKGQNIKNAKRLKFGDIYDEVLEKCKADGLSLKEIQRGLKYKNGVFKNFKDVDIESIKRSHVIETLKTIEHQIPTLIKAK
GWINKIFKYALQLEIVENNPVTSIDNSIVFKKASKVIHQPTLLENDKIKEYILALKNSDLKKTHKNLMLFNLLTAQRPGN
VIKATWDEIDLKNAIWTIKAEKMKMRKEHIITLNSQALKILKEQKKMKVNDYIFAGSSKNGCNSENITCNINKRLGYKGI
QSAHGFRAMFRSLANEHQLDHGVSMDIAEQCLAHEQKNAILKAYNRSENIELKRKLMQWWGDYIEKLAGDF
>Mature_390_residues
AKINKLTDSFLKSIKCESDKKFIKFADPSLKGLYVFIYPSGKKLFKIRQANDTYIKIGEYPLLSLAELREIALNAFKLKA
KGQNIKNAKRLKFGDIYDEVLEKCKADGLSLKEIQRGLKYKNGVFKNFKDVDIESIKRSHVIETLKTIEHQIPTLIKAKG
WINKIFKYALQLEIVENNPVTSIDNSIVFKKASKVIHQPTLLENDKIKEYILALKNSDLKKTHKNLMLFNLLTAQRPGNV
IKATWDEIDLKNAIWTIKAEKMKMRKEHIITLNSQALKILKEQKKMKVNDYIFAGSSKNGCNSENITCNINKRLGYKGIQ
SAHGFRAMFRSLANEHQLDHGVSMDIAEQCLAHEQKNAILKAYNRSENIELKRKLMQWWGDYIEKLAGDF

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=405, Percent_Identity=26.6666666666667, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788690, Length=369, Percent_Identity=26.2872628726287, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI145693166, Length=390, Percent_Identity=28.2051282051282, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45026; Mature: 44895

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKINKLTDSFLKSIKCESDKKFIKFADPSLKGLYVFIYPSGKKLFKIRQANDTYIKIGE
CCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEECC
YPLLSLAELREIALNAFKLKAKGQNIKNAKRLKFGDIYDEVLEKCKADGLSLKEIQRGLK
CCHHHHHHHHHHHHHHHEEEECCCCCCCHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
YKNGVFKNFKDVDIESIKRSHVIETLKTIEHQIPTLIKAKGWINKIFKYALQLEIVENNP
HCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
VTSIDNSIVFKKASKVIHQPTLLENDKIKEYILALKNSDLKKTHKNLMLFNLLTAQRPGN
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VIKATWDEIDLKNAIWTIKAEKMKMRKEHIITLNSQALKILKEQKKMKVNDYIFAGSSKN
EEEECHHHHHHCHHEEEEEHHHHHHHHHHEEEECHHHHHHHHHHHHCEECCEEEECCCCC
GCNSENITCNINKRLGYKGIQSAHGFRAMFRSLANEHQLDHGVSMDIAEQCLAHEQKNAI
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LKAYNRSENIELKRKLMQWWGDYIEKLAGDF
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKINKLTDSFLKSIKCESDKKFIKFADPSLKGLYVFIYPSGKKLFKIRQANDTYIKIGE
CCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEECC
YPLLSLAELREIALNAFKLKAKGQNIKNAKRLKFGDIYDEVLEKCKADGLSLKEIQRGLK
CCHHHHHHHHHHHHHHHEEEECCCCCCCHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
YKNGVFKNFKDVDIESIKRSHVIETLKTIEHQIPTLIKAKGWINKIFKYALQLEIVENNP
HCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
VTSIDNSIVFKKASKVIHQPTLLENDKIKEYILALKNSDLKKTHKNLMLFNLLTAQRPGN
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VIKATWDEIDLKNAIWTIKAEKMKMRKEHIITLNSQALKILKEQKKMKVNDYIFAGSSKN
EEEECHHHHHHCHHEEEEEHHHHHHHHHHEEEECHHHHHHHHHHHHCEECCEEEECCCCC
GCNSENITCNINKRLGYKGIQSAHGFRAMFRSLANEHQLDHGVSMDIAEQCLAHEQKNAI
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LKAYNRSENIELKRKLMQWWGDYIEKLAGDF
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]