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The map label for this gene is pnp [H]

Identifier: 57238127

GI number: 57238127

Start: 1304373

End: 1306532

Strand: Direct

Name: pnp [H]

Synonym: CJE1390

Alternate gene names: 57238127

Gene position: 1304373-1306532 (Clockwise)

Preceding gene: 57238126

Following gene: 57238128

Centisome position: 73.37

GC content: 36.2

Gene sequence:

>2160_bases
ATGCAATACAGCATAGAAATTAACAAAAATACAGAAATATTTAATATAGACAAAGTCGCCAAACAAGCTGCCGGTGCTGT
TTTAATGAGACAAGGCAAAAGCGTTGTTCTAGCTACAGTTGCAAGAGAAGAAAAACAAGTTGAAGAAGATTTTTTACCTC
TAACCGTCCAATACATCGAAAAGGCTTATGCTGCAGGAAAAATCCCTGGTGGTTATGTCAAAAGAGAAACCAAGCCAAGC
GATGCAGAAACCTTAACAGCACGCATTATTGATAGAAGCTTACGCCCTCTTTTTCCAAAAGGCTATGCTTATCCGACTCA
AATCGTTGTGATGGTTCTCTCAGCTGATCCTAAAGTAGATTTGCAAGTAATGAGCTTAAATGCCGCAAGCGTGGCACTTT
ATCTTAGTGATATACCTATGAAAGCTCCAGTATGTGGTGTGCGTATAGGAAAGATAGATGGAAATTTCATACTTAATCCA
AACAATGAAGAGCTTCAAAATAGCACCCTAGATCTCTATGTAGCAGGTGTAAAAGATGAGCTTTTGATGATAGAAATGAG
AGCCTTACCTGATCAAAAAGAAAATGAAATCTTTATCGAAGCTCCTTATGCTGATGTTTTAACTCAAACCACTTCACAAA
ATATGAATGAACTTAGCGAAGATGAAATTTTAGAAGCTCTAAATTTAGCTCAAAAAGCTATATTAAATGGTTCAAATGCT
TATGAAGAAGCCTTTAGCAAACACAAAAAAAATTCTCAAATCGAACTAAAAAATGAGATTGAATATCCTGAAATTTTAGC
TTTTATAGAAAATAATTTCCAAAAACAAATCAAAGAAGCCATCAACCAAATGGCAAAAAGCGAAAGAGCAAGCGAACTAA
ATAAAATCGCTAAAGAAATTTCAAATCTTGAAATCGCTAAAGAGTGGAGCGAAGAAAGTGTCTTAAATACCTTAGCTAAA
GTCAAACGCAAACTTATTCGTGGGCAAATTTTAAATGAAGGCAAAAGAGCTGATGGTAGAAGTTTAAACGAAGTGCGTCC
TATTAGTATAGAAACCAATATCTTGCCAAACGCTCATGGTTCTTGTCTTTTCACTCGTGGACAAACTCAAGCCCTAGTGG
TTGCAACCTTAGGCGGAGAAAACGATGCTCAAATGATAGACTTGCTTACAGAAAAAAATCCTATAAGCGAACGCTTTATG
GTAAATTATAATTTTCCAGGTTTTTCAGTAGGCGAAGCAAGTCCTATCAAAGCTCCAGGAAGAAGAGAACTAGGACATGG
AAATTTAGCCAAAAGAGCACTTTATCCAAGTGTGGATGAAAACTATCCTTATGTAATCCGCTTAGTCAGTGAAATTTTAG
AAAGCAATGGCTCAAGTTCTATGGCAACGGTTTGTGGCGGATCTTTAGCCTTAAAAGCAGCAGGAGTGCCAAGCTTAAAA
CTTGTTGCAGGAGTAGCCATGGGGCTTATCTTTGAAGATAACAAGTATGCTGTTTTAACAGATATCATGGGACTTGAAGA
TCATGATGGGGATATGGACTTTAAAGTAGCAGGAAGCAAAGACGGCGTTACTGCTCTTCAAATGGATATAAAACTAGGTG
GAATCGATCAAGAAACACTAAAACAAGCTTTATACCAAGCCAAAGAAGGAAGAATTCACATTCTAAATATCATGGAAGAA
GCCGCCAAAGAAATCATAGTCAATGAAGAAGTTTTACCTAAACTTGAGCTTTTTAGCGTTGATCCTTCAAAAATCGTTGA
TATTATAGGACAAGCCGGAAAAACCATCAAAGAAATCGTAGAAAAATTTGGCGTTTCTATAGACTTAGATCGCGAAAAAG
GTGAAGTAAAAATAGCAGGTAGTCAAAATGAGCAAATCAAAGCCGCAAAAGACTACATCATAAACATCACAAGCTCTCAA
AAAGGAACTAAAAAAGGCTCTAAAGACAAAGATATATCAGGCTTTGAACTTGGGCAAGAATTTCAAGGTATTGTTAAAAA
AATCGCTCCTTTTGGAGCCTTTGTGGAACTTAAAAATGGTGTTGATGGTTTATTGCATAGTTCAAAAAGCAAGCATCTTA
ATTTAAGCGAAAACCAAAGCTTAAAAGTTAAAATCAGCGAAATTAAAAACGGAAAAATTTCTGTGGATTTGTGTGAGTGA

Upstream 100 bases:

>100_bases
CTCTTTAGCAGAAAGTGAAAACAAAATTTAAAAAGGATGAAATTTGCTAAATATTTTAAAAAATTTTACGAATTTATTAT
CTGGTAAAAAAGGAAAAATA

Downstream 100 bases:

>100_bases
GTGAATTTCTAACTCATCCTTTTGAGCCTTTTTTTGACAAAGATTCTAAAATCTTAATTTTAGGATCTTTTCCTTCGGTA
AAATCAAGACAAGATGGCTT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MQYSIEINKNTEIFNIDKVAKQAAGAVLMRQGKSVVLATVAREEKQVEEDFLPLTVQYIEKAYAAGKIPGGYVKRETKPS
DAETLTARIIDRSLRPLFPKGYAYPTQIVVMVLSADPKVDLQVMSLNAASVALYLSDIPMKAPVCGVRIGKIDGNFILNP
NNEELQNSTLDLYVAGVKDELLMIEMRALPDQKENEIFIEAPYADVLTQTTSQNMNELSEDEILEALNLAQKAILNGSNA
YEEAFSKHKKNSQIELKNEIEYPEILAFIENNFQKQIKEAINQMAKSERASELNKIAKEISNLEIAKEWSEESVLNTLAK
VKRKLIRGQILNEGKRADGRSLNEVRPISIETNILPNAHGSCLFTRGQTQALVVATLGGENDAQMIDLLTEKNPISERFM
VNYNFPGFSVGEASPIKAPGRRELGHGNLAKRALYPSVDENYPYVIRLVSEILESNGSSSMATVCGGSLALKAAGVPSLK
LVAGVAMGLIFEDNKYAVLTDIMGLEDHDGDMDFKVAGSKDGVTALQMDIKLGGIDQETLKQALYQAKEGRIHILNIMEE
AAKEIIVNEEVLPKLELFSVDPSKIVDIIGQAGKTIKEIVEKFGVSIDLDREKGEVKIAGSQNEQIKAAKDYIINITSSQ
KGTKKGSKDKDISGFELGQEFQGIVKKIAPFGAFVELKNGVDGLLHSSKSKHLNLSENQSLKVKISEIKNGKISVDLCE

Sequences:

>Translated_719_residues
MQYSIEINKNTEIFNIDKVAKQAAGAVLMRQGKSVVLATVAREEKQVEEDFLPLTVQYIEKAYAAGKIPGGYVKRETKPS
DAETLTARIIDRSLRPLFPKGYAYPTQIVVMVLSADPKVDLQVMSLNAASVALYLSDIPMKAPVCGVRIGKIDGNFILNP
NNEELQNSTLDLYVAGVKDELLMIEMRALPDQKENEIFIEAPYADVLTQTTSQNMNELSEDEILEALNLAQKAILNGSNA
YEEAFSKHKKNSQIELKNEIEYPEILAFIENNFQKQIKEAINQMAKSERASELNKIAKEISNLEIAKEWSEESVLNTLAK
VKRKLIRGQILNEGKRADGRSLNEVRPISIETNILPNAHGSCLFTRGQTQALVVATLGGENDAQMIDLLTEKNPISERFM
VNYNFPGFSVGEASPIKAPGRRELGHGNLAKRALYPSVDENYPYVIRLVSEILESNGSSSMATVCGGSLALKAAGVPSLK
LVAGVAMGLIFEDNKYAVLTDIMGLEDHDGDMDFKVAGSKDGVTALQMDIKLGGIDQETLKQALYQAKEGRIHILNIMEE
AAKEIIVNEEVLPKLELFSVDPSKIVDIIGQAGKTIKEIVEKFGVSIDLDREKGEVKIAGSQNEQIKAAKDYIINITSSQ
KGTKKGSKDKDISGFELGQEFQGIVKKIAPFGAFVELKNGVDGLLHSSKSKHLNLSENQSLKVKISEIKNGKISVDLCE
>Mature_719_residues
MQYSIEINKNTEIFNIDKVAKQAAGAVLMRQGKSVVLATVAREEKQVEEDFLPLTVQYIEKAYAAGKIPGGYVKRETKPS
DAETLTARIIDRSLRPLFPKGYAYPTQIVVMVLSADPKVDLQVMSLNAASVALYLSDIPMKAPVCGVRIGKIDGNFILNP
NNEELQNSTLDLYVAGVKDELLMIEMRALPDQKENEIFIEAPYADVLTQTTSQNMNELSEDEILEALNLAQKAILNGSNA
YEEAFSKHKKNSQIELKNEIEYPEILAFIENNFQKQIKEAINQMAKSERASELNKIAKEISNLEIAKEWSEESVLNTLAK
VKRKLIRGQILNEGKRADGRSLNEVRPISIETNILPNAHGSCLFTRGQTQALVVATLGGENDAQMIDLLTEKNPISERFM
VNYNFPGFSVGEASPIKAPGRRELGHGNLAKRALYPSVDENYPYVIRLVSEILESNGSSSMATVCGGSLALKAAGVPSLK
LVAGVAMGLIFEDNKYAVLTDIMGLEDHDGDMDFKVAGSKDGVTALQMDIKLGGIDQETLKQALYQAKEGRIHILNIMEE
AAKEIIVNEEVLPKLELFSVDPSKIVDIIGQAGKTIKEIVEKFGVSIDLDREKGEVKIAGSQNEQIKAAKDYIINITSSQ
KGTKKGSKDKDISGFELGQEFQGIVKKIAPFGAFVELKNGVDGLLHSSKSKHLNLSENQSLKVKISEIKNGKISVDLCE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=734, Percent_Identity=35.6948228882834, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI145693187, Length=734, Percent_Identity=38.8283378746594, Blast_Score=482, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI115534063, Length=635, Percent_Identity=33.8582677165354, Blast_Score=332, Evalue=5e-91,
Organism=Drosophila melanogaster, GI281362905, Length=626, Percent_Identity=36.1022364217252, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24651641, Length=626, Percent_Identity=36.1022364217252, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24651643, Length=626, Percent_Identity=36.1022364217252, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI161079377, Length=564, Percent_Identity=36.7021276595745, Blast_Score=346, Evalue=4e-95,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79019; Mature: 79019

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYSIEINKNTEIFNIDKVAKQAAGAVLMRQGKSVVLATVAREEKQVEEDFLPLTVQYIE
CEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCHHHHHHH
KAYAAGKIPGGYVKRETKPSDAETLTARIIDRSLRPLFPKGYAYPTQIVVMVLSADPKVD
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCE
LQVMSLNAASVALYLSDIPMKAPVCGVRIGKIDGNFILNPNNEELQNSTLDLYVAGVKDE
EEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHCCCCEEEEEECCCCC
LLMIEMRALPDQKENEIFIEAPYADVLTQTTSQNMNELSEDEILEALNLAQKAILNGSNA
EEEEEEHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
YEEAFSKHKKNSQIELKNEIEYPEILAFIENNFQKQIKEAINQMAKSERASELNKIAKEI
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNLEIAKEWSEESVLNTLAKVKRKLIRGQILNEGKRADGRSLNEVRPISIETNILPNAHG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
SCLFTRGQTQALVVATLGGENDAQMIDLLTEKNPISERFMVNYNFPGFSVGEASPIKAPG
CEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
RRELGHGNLAKRALYPSVDENYPYVIRLVSEILESNGSSSMATVCGGSLALKAAGVPSLK
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCCHHH
LVAGVAMGLIFEDNKYAVLTDIMGLEDHDGDMDFKVAGSKDGVTALQMDIKLGGIDQETL
HHHHHHHHEEEECCCEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCHHHH
KQALYQAKEGRIHILNIMEEAAKEIIVNEEVLPKLELFSVDPSKIVDIIGQAGKTIKEIV
HHHHHHHCCCCEEEHHHHHHHHHHHHCCHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHH
EKFGVSIDLDREKGEVKIAGSQNEQIKAAKDYIINITSSQKGTKKGSKDKDISGFELGQE
HHHCCEEEEECCCCCEEEECCCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHH
FQGIVKKIAPFGAFVELKNGVDGLLHSSKSKHLNLSENQSLKVKISEIKNGKISVDLCE
HHHHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MQYSIEINKNTEIFNIDKVAKQAAGAVLMRQGKSVVLATVAREEKQVEEDFLPLTVQYIE
CEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCHHHHHHH
KAYAAGKIPGGYVKRETKPSDAETLTARIIDRSLRPLFPKGYAYPTQIVVMVLSADPKVD
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCE
LQVMSLNAASVALYLSDIPMKAPVCGVRIGKIDGNFILNPNNEELQNSTLDLYVAGVKDE
EEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHCCCCEEEEEECCCCC
LLMIEMRALPDQKENEIFIEAPYADVLTQTTSQNMNELSEDEILEALNLAQKAILNGSNA
EEEEEEHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
YEEAFSKHKKNSQIELKNEIEYPEILAFIENNFQKQIKEAINQMAKSERASELNKIAKEI
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNLEIAKEWSEESVLNTLAKVKRKLIRGQILNEGKRADGRSLNEVRPISIETNILPNAHG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
SCLFTRGQTQALVVATLGGENDAQMIDLLTEKNPISERFMVNYNFPGFSVGEASPIKAPG
CEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
RRELGHGNLAKRALYPSVDENYPYVIRLVSEILESNGSSSMATVCGGSLALKAAGVPSLK
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCCHHH
LVAGVAMGLIFEDNKYAVLTDIMGLEDHDGDMDFKVAGSKDGVTALQMDIKLGGIDQETL
HHHHHHHHEEEECCCEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCHHHH
KQALYQAKEGRIHILNIMEEAAKEIIVNEEVLPKLELFSVDPSKIVDIIGQAGKTIKEIV
HHHHHHHCCCCEEEHHHHHHHHHHHHCCHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHH
EKFGVSIDLDREKGEVKIAGSQNEQIKAAKDYIINITSSQKGTKKGSKDKDISGFELGQE
HHHCCEEEEECCCCCEEEECCCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHH
FQGIVKKIAPFGAFVELKNGVDGLLHSSKSKHLNLSENQSLKVKISEIKNGKISVDLCE
HHHHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA