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The map label for this gene is purD

Identifier: 57238124

GI number: 57238124

Start: 1300566

End: 1301816

Strand: Direct

Name: purD

Synonym: CJE1387

Alternate gene names: 57238124

Gene position: 1300566-1301816 (Clockwise)

Preceding gene: 57238123

Following gene: 57238125

Centisome position: 73.15

GC content: 34.29

Gene sequence:

>1251_bases
ATGAAAATAATGATTTTGGGTAGTGGTGCAAGAGAATATTCTATTGCTTTAGCCTTAAGAAGAGTAGACAAAAATTTAGA
GTTTTATTTTGCTCCTGGAAATGGCGCTACCGAAAGTCTTGGAACAAATTTAAACCTAAAAGATCCAGTAGTTTTAGCAA
CCTATGCAAAAGAAAAAGGTTTTGATCTTTGTATAGTAGGAAGTGAAAGTTTTTTAGCTGAAGGGGTTGTAGATATTTTT
AAACAACAAGGACTTGCTATTTTTGGGCCAAGTAAAGCTGCTGCAATGCTTGAAACCTCAAAATCTTTTATGAAAAGCTT
TCTTAAAAAATACCGTATCAAAACAGCCAAATTTTTAAACACAAATGACATTGAAAAAGCAAAAAATTTTATATATAGTT
TAACCCCTCCTATAGTAGTAAAAGCCGATGGACTTTGCGCAGGAAAAGGTGTTATCATAGCTAAAACTCATGAAGAAGCC
ATAGAAGAAACAGCTAAAATGCTGAGCGGAGAAAGCTTTGGAGATGCTGGAAAACTCGTTGTAATTGAAGAATTTTTAGA
TGGTTATGAACTAAGCATTTTTGCAGTATGTGATGGCAATGATTTTGTTTTACTTCCTGCAGCACAAGATCACAAAAAAT
TACTCGATAATGATCAAGGGCCAAATACTGGTGGAATGGGTGCTTACGCTCCTAGCTCTTTGGCGAATGAAAGCTTGCTT
AGAAAAGTTCAAAAAGATATTATTTTACCAACTTTAGCAGGTATGAAAAAAGAAGGAGCGGAATTTTGTGGTGTTTTATT
TATAGGTGCTATGATCGTTGGAAATAAACCTTACGTATTAGAATTTAACGTTCGCTTTGGCGATCCTGAATGTGAAGTTT
TAATGCCTTTAATCGAAGATCCTTTAGAGCTTATTTTAGCTGCTACTCAAAGACGCTTAAGACACTCAAAAATCAAAATC
AAAAAAGAATTTGCAGTAGGGGTTGTATGCGCAAGTGAAAATTATCCTTACAAAAGTTCACCAAAAAGTGAAATTACCGT
AAATAACATTCCTGAAAACTCTCATATTTCTTATGCTGGAGTAAGCCTAGAAGATGGCAAATTAATGGCTGATGGTGGAA
GAGTTTTAGTTTGCGTTGGCACAGGAAAAAGTATAGAAGAAGCGCAAAAAAATGCTTATAAACTTTGTGATAATGTAAAT
TTCAAAGGAAAACAATACAGAAAAGACATCGCTCATCAGGTTCTGAAATGA

Upstream 100 bases:

>100_bases
AGTGAGAAGTTGCAGCCTTTAAAAATTACCTAGCGGTTTTCTTTGACTTTTTGGGGTCAATCTTTGTTAAACGGCTGTTT
GGGTTTAAAAGGAGATATAA

Downstream 100 bases:

>100_bases
AAGAAAATTTACAAGACCGCTTAGAGCGTGAAAATCTAAAAATAGCAAGTTTTGGAAAAAGAGTTCTAGCTTTTTTAATC
GATGATATGGTTATTTCTTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKIMILGSGAREYSIALALRRVDKNLEFYFAPGNGATESLGTNLNLKDPVVLATYAKEKGFDLCIVGSESFLAEGVVDIF
KQQGLAIFGPSKAAAMLETSKSFMKSFLKKYRIKTAKFLNTNDIEKAKNFIYSLTPPIVVKADGLCAGKGVIIAKTHEEA
IEETAKMLSGESFGDAGKLVVIEEFLDGYELSIFAVCDGNDFVLLPAAQDHKKLLDNDQGPNTGGMGAYAPSSLANESLL
RKVQKDIILPTLAGMKKEGAEFCGVLFIGAMIVGNKPYVLEFNVRFGDPECEVLMPLIEDPLELILAATQRRLRHSKIKI
KKEFAVGVVCASENYPYKSSPKSEITVNNIPENSHISYAGVSLEDGKLMADGGRVLVCVGTGKSIEEAQKNAYKLCDNVN
FKGKQYRKDIAHQVLK

Sequences:

>Translated_416_residues
MKIMILGSGAREYSIALALRRVDKNLEFYFAPGNGATESLGTNLNLKDPVVLATYAKEKGFDLCIVGSESFLAEGVVDIF
KQQGLAIFGPSKAAAMLETSKSFMKSFLKKYRIKTAKFLNTNDIEKAKNFIYSLTPPIVVKADGLCAGKGVIIAKTHEEA
IEETAKMLSGESFGDAGKLVVIEEFLDGYELSIFAVCDGNDFVLLPAAQDHKKLLDNDQGPNTGGMGAYAPSSLANESLL
RKVQKDIILPTLAGMKKEGAEFCGVLFIGAMIVGNKPYVLEFNVRFGDPECEVLMPLIEDPLELILAATQRRLRHSKIKI
KKEFAVGVVCASENYPYKSSPKSEITVNNIPENSHISYAGVSLEDGKLMADGGRVLVCVGTGKSIEEAQKNAYKLCDNVN
FKGKQYRKDIAHQVLK
>Mature_416_residues
MKIMILGSGAREYSIALALRRVDKNLEFYFAPGNGATESLGTNLNLKDPVVLATYAKEKGFDLCIVGSESFLAEGVVDIF
KQQGLAIFGPSKAAAMLETSKSFMKSFLKKYRIKTAKFLNTNDIEKAKNFIYSLTPPIVVKADGLCAGKGVIIAKTHEEA
IEETAKMLSGESFGDAGKLVVIEEFLDGYELSIFAVCDGNDFVLLPAAQDHKKLLDNDQGPNTGGMGAYAPSSLANESLL
RKVQKDIILPTLAGMKKEGAEFCGVLFIGAMIVGNKPYVLEFNVRFGDPECEVLMPLIEDPLELILAATQRRLRHSKIKI
KKEFAVGVVCASENYPYKSSPKSEITVNNIPENSHISYAGVSLEDGKLMADGGRVLVCVGTGKSIEEAQKNAYKLCDNVN
FKGKQYRKDIAHQVLK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=38.8235294117647, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=38.8235294117647, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=38.8235294117647, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=38.8235294117647, Blast_Score=298, Evalue=8e-81,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=39.2523364485981, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=40.0473933649289, Blast_Score=283, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=37.7272727272727, Blast_Score=286, Evalue=3e-78,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=37.122969837587, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=36.8909512761021, Blast_Score=251, Evalue=7e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_CAMJE (Q9PN47)

Other databases:

- EMBL:   AL111168
- PIR:   D81332
- RefSeq:   YP_002344641.1
- ProteinModelPortal:   Q9PN47
- SMR:   Q9PN47
- IntAct:   Q9PN47
- MINT:   MINT-6484378
- GeneID:   905541
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj1250
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   CJEJ192222:CJ1250-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 45316; Mature: 45316

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIMILGSGAREYSIALALRRVDKNLEFYFAPGNGATESLGTNLNLKDPVVLATYAKEKG
CEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCC
FDLCIVGSESFLAEGVVDIFKQQGLAIFGPSKAAAMLETSKSFMKSFLKKYRIKTAKFLN
CEEEEECCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TNDIEKAKNFIYSLTPPIVVKADGLCAGKGVIIAKTHEEAIEETAKMLSGESFGDAGKLV
CCHHHHHHHHEEECCCCEEEEECCEECCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEE
VIEEFLDGYELSIFAVCDGNDFVLLPAAQDHKKLLDNDQGPNTGGMGAYAPSSLANESLL
EEEHHHCCCEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
RKVQKDIILPTLAGMKKEGAEFCGVLFIGAMIVGNKPYVLEFNVRFGDPECEVLMPLIED
HHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHH
PLELILAATQRRLRHSKIKIKKEFAVGVVCASENYPYKSSPKSEITVNNIPENSHISYAG
HHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEE
VSLEDGKLMADGGRVLVCVGTGKSIEEAQKNAYKLCDNVNFKGKQYRKDIAHQVLK
EEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIMILGSGAREYSIALALRRVDKNLEFYFAPGNGATESLGTNLNLKDPVVLATYAKEKG
CEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCC
FDLCIVGSESFLAEGVVDIFKQQGLAIFGPSKAAAMLETSKSFMKSFLKKYRIKTAKFLN
CEEEEECCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TNDIEKAKNFIYSLTPPIVVKADGLCAGKGVIIAKTHEEAIEETAKMLSGESFGDAGKLV
CCHHHHHHHHEEECCCCEEEEECCEECCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEE
VIEEFLDGYELSIFAVCDGNDFVLLPAAQDHKKLLDNDQGPNTGGMGAYAPSSLANESLL
EEEHHHCCCEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
RKVQKDIILPTLAGMKKEGAEFCGVLFIGAMIVGNKPYVLEFNVRFGDPECEVLMPLIED
HHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHH
PLELILAATQRRLRHSKIKIKKEFAVGVVCASENYPYKSSPKSEITVNNIPENSHISYAG
HHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEE
VSLEDGKLMADGGRVLVCVGTGKSIEEAQKNAYKLCDNVNFKGKQYRKDIAHQVLK
EEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204