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The map label for this gene is metF [H]

Identifier: 57238074

GI number: 57238074

Start: 1253080

End: 1253928

Strand: Direct

Name: metF [H]

Synonym: CJE1336

Alternate gene names: 57238074

Gene position: 1253080-1253928 (Clockwise)

Preceding gene: 57238073

Following gene: 57238080

Centisome position: 70.48

GC content: 30.39

Gene sequence:

>849_bases
ATGTGTAGTTTTTCTTTTGAAGTTTTTCCACCAAGAAAGGATGAAAATATCAAAAATCTTCATGCTATCTTAGATGATTT
AGGGCAATTAAGCCCTAATTTTATCAGCGTAACCTTTGGAGCTGGAGGCTCTATTAACTCACAAAATACTTTAGAAGTTG
CAAGCTTAATCCAGGAAGAATATCAAATTCCTAGCATAGTGCATTTACCTTGCATCCATTCTAGTAAAGAAAAAATCACT
CAGATACTTCAAAAATGCAAAGAAAAAAATCTTAATCAAATTCTTACTCTAAGAGGCGATATATGTGAAAATTTAGAAAA
AAGTAAAGATTTTTCTTATGCTAGTGATTTAATTTCTTTTATAAAAAAACAAGAATACTTTGAAATTTATGCCGCATGCT
ATCCTGAAAAACATAATGAATCTAAAAATTTCATCGAGGATATACACCATCTTAAAACTAAGGTAAATGCAGGAACAGAC
AAGCTCATTACTCAACTTTTTTACGATAATGAAGATTTTTATACTTTTAAACAAAATTGTGCTTTAGCAGGTATTGACAT
ACCTATTTACGCAGGTATTATGCCTATTACTAACAAAAGACAGGTTTTAAAAATTTCTCAACTTTGCGGAGCTAAAATCC
CTCCTAAATTTGTTAAAATTTTAGAAAAATATGAAAATAATGCTTTGGCTTTAGAAGATGCAGGTATCGCTTATGCTTGC
GATCAAATTGTCGATTTAATCACAAGTGGCGTAGATGGAATTCATCTTTATACTATGAATAAATCCAAAGCGGCTATTAA
AATTTATGAAGCTGTAAAGCATTTGCTTAAAGAAGAGCTTCATGCTTAG

Upstream 100 bases:

>100_bases
CTGCGGACTTAAAACAAGAGCCTATGAAGAAGTTATAGCCTCTCTTAAAAATTTAGTTACGGCTACGCAAAAAATACGAG
AACAATTATAAGGAGTTTTC

Downstream 100 bases:

>100_bases
AAAATAAACTATTTTTTGCTTTTATCTTAACTTATTTTCATTTTTTAGCTTATGGTTTTCTATAAGTAAAACAAAAATTA
AAGATAAAAGCAAACCTGGT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MCSFSFEVFPPRKDENIKNLHAILDDLGQLSPNFISVTFGAGGSINSQNTLEVASLIQEEYQIPSIVHLPCIHSSKEKIT
QILQKCKEKNLNQILTLRGDICENLEKSKDFSYASDLISFIKKQEYFEIYAACYPEKHNESKNFIEDIHHLKTKVNAGTD
KLITQLFYDNEDFYTFKQNCALAGIDIPIYAGIMPITNKRQVLKISQLCGAKIPPKFVKILEKYENNALALEDAGIAYAC
DQIVDLITSGVDGIHLYTMNKSKAAIKIYEAVKHLLKEELHA

Sequences:

>Translated_282_residues
MCSFSFEVFPPRKDENIKNLHAILDDLGQLSPNFISVTFGAGGSINSQNTLEVASLIQEEYQIPSIVHLPCIHSSKEKIT
QILQKCKEKNLNQILTLRGDICENLEKSKDFSYASDLISFIKKQEYFEIYAACYPEKHNESKNFIEDIHHLKTKVNAGTD
KLITQLFYDNEDFYTFKQNCALAGIDIPIYAGIMPITNKRQVLKISQLCGAKIPPKFVKILEKYENNALALEDAGIAYAC
DQIVDLITSGVDGIHLYTMNKSKAAIKIYEAVKHLLKEELHA
>Mature_282_residues
MCSFSFEVFPPRKDENIKNLHAILDDLGQLSPNFISVTFGAGGSINSQNTLEVASLIQEEYQIPSIVHLPCIHSSKEKIT
QILQKCKEKNLNQILTLRGDICENLEKSKDFSYASDLISFIKKQEYFEIYAACYPEKHNESKNFIEDIHHLKTKVNAGTD
KLITQLFYDNEDFYTFKQNCALAGIDIPIYAGIMPITNKRQVLKISQLCGAKIPPKFVKILEKYENNALALEDAGIAYAC
DQIVDLITSGVDGIHLYTMNKSKAAIKIYEAVKHLLKEELHA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=276, Percent_Identity=35.8695652173913, Blast_Score=163, Evalue=1e-40,
Organism=Escherichia coli, GI1790377, Length=264, Percent_Identity=33.3333333333333, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=29.4964028776978, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=29.4964028776978, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6325234, Length=286, Percent_Identity=35.6643356643357, Blast_Score=152, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6321313, Length=286, Percent_Identity=31.8181818181818, Blast_Score=135, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24662658, Length=265, Percent_Identity=23.7735849056604, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31868; Mature: 31868

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSFSFEVFPPRKDENIKNLHAILDDLGQLSPNFISVTFGAGGSINSQNTLEVASLIQEE
CCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
YQIPSIVHLPCIHSSKEKITQILQKCKEKNLNQILTLRGDICENLEKSKDFSYASDLISF
HCCCCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IKKQEYFEIYAACYPEKHNESKNFIEDIHHLKTKVNAGTDKLITQLFYDNEDFYTFKQNC
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCC
ALAGIDIPIYAGIMPITNKRQVLKISQLCGAKIPPKFVKILEKYENNALALEDAGIAYAC
EEECCCCHHHHCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHH
DQIVDLITSGVDGIHLYTMNKSKAAIKIYEAVKHLLKEELHA
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCSFSFEVFPPRKDENIKNLHAILDDLGQLSPNFISVTFGAGGSINSQNTLEVASLIQEE
CCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
YQIPSIVHLPCIHSSKEKITQILQKCKEKNLNQILTLRGDICENLEKSKDFSYASDLISF
HCCCCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IKKQEYFEIYAACYPEKHNESKNFIEDIHHLKTKVNAGTDKLITQLFYDNEDFYTFKQNC
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCC
ALAGIDIPIYAGIMPITNKRQVLKISQLCGAKIPPKFVKILEKYENNALALEDAGIAYAC
EEECCCCHHHHCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHH
DQIVDLITSGVDGIHLYTMNKSKAAIKIYEAVKHLLKEELHA
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]