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The map label for this gene is dapE [H]

Identifier: 57237934

GI number: 57237934

Start: 1108813

End: 1109910

Strand: Reverse

Name: dapE [H]

Synonym: CJE1192

Alternate gene names: 57237934

Gene position: 1109910-1108813 (Counterclockwise)

Preceding gene: 57237935

Following gene: 57237933

Centisome position: 62.43

GC content: 36.07

Gene sequence:

>1098_bases
ATGAATGCAAAAGAATTTTTAATCGAACTTTTAAAATTTAAATCTGTTACGCCAAATGATGATGGAGCTTTAAATTTCAT
CGCTATGGAACTGAGTGATTTTGAAGCTTTTTTTATAGAAAAAGAAGGCATTAAAAACCTTTTACTCACTAAAAAATTTA
AAGATGAAGGCGAGCATTTAGCTTTTGGTGGGCATGTAGATGTGGTGCCTGCAGGTGAGGGTTGGAGTAGCGATGCTTTT
GTGCCTATGGAAAAAGAAGGCTTTATCTATGCAAGGGGTGCTCAAGATATGAAAAGCGGAGTAGCAGCCTTTGTAGATGC
GGTAAAGAATGCGGATTTTAAAGGAGCTAGACTTAGCCTTATTTTAACAAGTGATGAAGAAGGCGAAGCTATATATGGAA
CTAAGGCTGTTTTAGAATGGATGCAAGAAAGAGATATGCTACCTGATTATGCTGTGGTTGCCGAGCCAACCTGCGTTAAA
AAAATCGGCGATAGTATTAAAATAGGGCGTCGTGGTTCTATCAATGGAAAACTTTTAATCCGTGGAAAACAAGGGCATGC
GGCTTACCCTGAAAAATGTATCAATCCTGTGCATGATTTTGCGCCAGTTTTAAAGCTTTTAGCGGGTTTTGATCTTGATC
CTGGAAGTGCTGAATTTAGTCCTTCAAAAATCGTTATAACTGATATTCGCGGAGGTATGGGGGTGTGTAATGTTACGCCA
AATGATTTAAAACTTATGTTTAATGTTCGCAATTCCCCTGATACAAGCTTAGAAGATGTAAAAAGTTATGTAGAAAAAAT
TTGTCATGGTTTAAACTATGAGTTAGAGCTAAAACAATCAAGCGAAGCTTTTTTGACAAATATCGATAATAAAATCGTTC
AAAAAATGAATGAAAGCGTTCAAAAAATCACCCATGAAGTGCCAGAGCTTAATACTAAAGGCGGCACAAGTGATGCAAGG
TATTTTGCAAAATACGGTGTAAAGGTAGTGGAATTTGGGGTTTGTAATGATAGAATTCACGCTATTGATGAAAGAGTAAG
TATAGAAGAATTTGAAAAACTTTGCCTTGTTTTTAAAGACTTGATAGAAAATTTTTAA

Upstream 100 bases:

>100_bases
TTTAGTGCAAAAGTTGTTTTTATAATCAATATAATTTCAGCTATAATTATAGAGTATTTTGCTCTTAATTTATTATATAA
AACTTTTATAGGATAAAAAA

Downstream 100 bases:

>100_bases
AGGTTTAGAATGATTATCAATGGACAAAAGCTTGAGTTAAAAGAGCTTAAATTTATGGATTTTATCAAAGAAAAAGGTTT
TAAAATCGAACTTATCGCCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHLAFGGHVDVVPAGEGWSSDAF
VPMEKEGFIYARGAQDMKSGVAAFVDAVKNADFKGARLSLILTSDEEGEAIYGTKAVLEWMQERDMLPDYAVVAEPTCVK
KIGDSIKIGRRGSINGKLLIRGKQGHAAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVITDIRGGMGVCNVTP
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESVQKITHEVPELNTKGGTSDAR
YFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKDLIENF

Sequences:

>Translated_365_residues
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHLAFGGHVDVVPAGEGWSSDAF
VPMEKEGFIYARGAQDMKSGVAAFVDAVKNADFKGARLSLILTSDEEGEAIYGTKAVLEWMQERDMLPDYAVVAEPTCVK
KIGDSIKIGRRGSINGKLLIRGKQGHAAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVITDIRGGMGVCNVTP
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESVQKITHEVPELNTKGGTSDAR
YFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKDLIENF
>Mature_365_residues
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHLAFGGHVDVVPAGEGWSSDAF
VPMEKEGFIYARGAQDMKSGVAAFVDAVKNADFKGARLSLILTSDEEGEAIYGTKAVLEWMQERDMLPDYAVVAEPTCVK
KIGDSIKIGRRGSINGKLLIRGKQGHAAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVITDIRGGMGVCNVTP
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESVQKITHEVPELNTKGGTSDAR
YFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKDLIENF

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=369, Percent_Identity=35.230352303523, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1790395, Length=141, Percent_Identity=30.4964539007092, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40492; Mature: 40492

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHL
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCEE
AFGGHVDVVPAGEGWSSDAFVPMEKEGFIYARGAQDMKSGVAAFVDAVKNADFKGARLSL
EECCEEEEEECCCCCCCCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEE
ILTSDEEGEAIYGTKAVLEWMQERDMLPDYAVVAEPTCVKKIGDSIKIGRRGSINGKLLI
EEECCCCCCEEEHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCEECCCCCCCCCEEEE
RGKQGHAAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVITDIRGGMGVCNVTP
EECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEECCC
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESV
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
QKITHEVPELNTKGGTSDARYFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKD
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHH
LIENF
HHHCC
>Mature Secondary Structure
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHL
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCEE
AFGGHVDVVPAGEGWSSDAFVPMEKEGFIYARGAQDMKSGVAAFVDAVKNADFKGARLSL
EECCEEEEEECCCCCCCCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEE
ILTSDEEGEAIYGTKAVLEWMQERDMLPDYAVVAEPTCVKKIGDSIKIGRRGSINGKLLI
EEECCCCCCEEEHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCEECCCCCCCCCEEEE
RGKQGHAAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVITDIRGGMGVCNVTP
EECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEECCC
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESV
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
QKITHEVPELNTKGGTSDARYFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKD
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHH
LIENF
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA