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The map label for this gene is prsA

Identifier: 57237746

GI number: 57237746

Start: 932824

End: 933753

Strand: Reverse

Name: prsA

Synonym: CJE0996

Alternate gene names: 57237746

Gene position: 933753-932824 (Counterclockwise)

Preceding gene: 57237747

Following gene: 57237745

Centisome position: 52.52

GC content: 35.16

Gene sequence:

>930_bases
ATGCGAGGTTATAAAATTTTTTCAGGCTCAGCTAATGTCGAATTTGCAAGACAAGTTTCTAAATATCTCTCCCTGCCTTT
AAGTGATGCTGGAGTAAAGCGGTTTAGTGATGGAGAGATTAGCGTTCAAATTGATGAGAGTGTACGCGGAAAGGATGTTT
TTATTATTCAAAGTACTTGCGCTCCTACAAACGATAATTTAATGGAACTTTTAATTCTTACAGATGCTTTGCGTCGCTCA
AGTGCAAATTCAATTACGGCTATTATCCCATATTTTGGCTATGCGAGACAAGATAGAAAAGCAAATCCTAGAGTGCCAAT
TACTGCTAAACTTGTGGCTAATTTGATTCAAGCAGCTGGGATTGATCGCGTAGCTACGATAGATTTGCATGCAGGACAAA
TTCAGGGTTTTTTTGATATTCCAGTAGATAATCTTTATGGAAGTATAGTTTTTAATGATTATATTAAAGCCAAACATTTT
AAAAATGCTATTATAGGAAGTCCAGATATAGGGGGTGTTGCAAGAGCTAGGAGTGTTGCAAAGCATTTAGGACTTGATAT
AGTTATAGTTGATAAGCGTCGTGAAAAAGCCAATGAAAGTGAAGTAATGAATATTATTGGAGATGTAAAAGATAAAGAAG
TTATCTTAGTGGATGATATTATTGATACTGCTGGCACTATAGTTAAAGCCGCGGAAGCTTTAAAAGAAAAAGGTGCAAAA
TCTGTTATGGCTTGCTGTACTCATGCAGTTTTAAGTGGAAAAGCCTATGAGAGAATAGCAAGTGGAGTTTTAGATGAGCT
TGTGGTAACAGATACTATACCTTTAAAAGAGCAATTACCAAATATTAAAGTATTAAGCGTTACACCTGTTTTTGCTGAAG
TAATACGTCGTGTTTATCATAACGAAAGTGTAAATTCTCTCTTTATTTAG

Upstream 100 bases:

>100_bases
TATATTTTTATATAAGCTTTTTAAATTTACAGCAGTTTTTAACATTTTTTTTATTAAAATCTTTTGTATTAATTCATAAT
TTTATTGATTAAGGTTTTTT

Downstream 100 bases:

>100_bases
ATTAAAAAAGCTTAAAGATTATTTTTAAGCTTTTCATTTTTATGCTACTTAAAGAAACAAAAAACATATATTTTTTTAAA
AATGTGAAAAAACTAAACAT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRGYKIFSGSANVEFARQVSKYLSLPLSDAGVKRFSDGEISVQIDESVRGKDVFIIQSTCAPTNDNLMELLILTDALRRS
SANSITAIIPYFGYARQDRKANPRVPITAKLVANLIQAAGIDRVATIDLHAGQIQGFFDIPVDNLYGSIVFNDYIKAKHF
KNAIIGSPDIGGVARARSVAKHLGLDIVIVDKRREKANESEVMNIIGDVKDKEVILVDDIIDTAGTIVKAAEALKEKGAK
SVMACCTHAVLSGKAYERIASGVLDELVVTDTIPLKEQLPNIKVLSVTPVFAEVIRRVYHNESVNSLFI

Sequences:

>Translated_309_residues
MRGYKIFSGSANVEFARQVSKYLSLPLSDAGVKRFSDGEISVQIDESVRGKDVFIIQSTCAPTNDNLMELLILTDALRRS
SANSITAIIPYFGYARQDRKANPRVPITAKLVANLIQAAGIDRVATIDLHAGQIQGFFDIPVDNLYGSIVFNDYIKAKHF
KNAIIGSPDIGGVARARSVAKHLGLDIVIVDKRREKANESEVMNIIGDVKDKEVILVDDIIDTAGTIVKAAEALKEKGAK
SVMACCTHAVLSGKAYERIASGVLDELVVTDTIPLKEQLPNIKVLSVTPVFAEVIRRVYHNESVNSLFI
>Mature_309_residues
MRGYKIFSGSANVEFARQVSKYLSLPLSDAGVKRFSDGEISVQIDESVRGKDVFIIQSTCAPTNDNLMELLILTDALRRS
SANSITAIIPYFGYARQDRKANPRVPITAKLVANLIQAAGIDRVATIDLHAGQIQGFFDIPVDNLYGSIVFNDYIKAKHF
KNAIIGSPDIGGVARARSVAKHLGLDIVIVDKRREKANESEVMNIIGDVKDKEVILVDDIIDTAGTIVKAAEALKEKGAK
SVMACCTHAVLSGKAYERIASGVLDELVVTDTIPLKEQLPNIKVLSVTPVFAEVIRRVYHNESVNSLFI

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=47.2843450479233, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI4506129, Length=308, Percent_Identity=48.0519480519481, Blast_Score=291, Evalue=4e-79,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=47.2843450479233, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI84875539, Length=311, Percent_Identity=47.588424437299, Blast_Score=287, Evalue=9e-78,
Organism=Homo sapiens, GI4506133, Length=345, Percent_Identity=35.9420289855072, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=35.7558139534884, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI310128524, Length=141, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI310115209, Length=141, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI310118259, Length=141, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI310119946, Length=141, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1787458, Length=313, Percent_Identity=56.2300319488818, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=46.0063897763578, Blast_Score=283, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=46.0063897763578, Blast_Score=283, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=313, Percent_Identity=46.0063897763578, Blast_Score=282, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=46.1038961038961, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=36.3905325443787, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319403, Length=308, Percent_Identity=49.3506493506493, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6320946, Length=307, Percent_Identity=47.2312703583062, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6321776, Length=309, Percent_Identity=47.8964401294498, Blast_Score=271, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6322667, Length=200, Percent_Identity=42, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=109, Percent_Identity=39.4495412844037, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=48.2428115015974, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=44.9404761904762, Blast_Score=276, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24651458, Length=351, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24651456, Length=351, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI281362873, Length=351, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24651454, Length=351, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24651462, Length=199, Percent_Identity=36.1809045226131, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24651464, Length=199, Percent_Identity=36.1809045226131, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45552010, Length=199, Percent_Identity=36.1809045226131, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_CAMJE (Q9PP15)

Other databases:

- EMBL:   AL111168
- PIR:   E81365
- RefSeq:   YP_002344316.1
- ProteinModelPortal:   Q9PP15
- SMR:   Q9PP15
- IntAct:   Q9PP15
- GeneID:   905217
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj0918c
- HOGENOM:   HBG519284
- OMA:   CATHAVF
- PhylomeDB:   Q9PP15
- ProtClustDB:   PRK01259
- BioCyc:   CJEJ192222:CJ0918C-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 33698; Mature: 33698

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGYKIFSGSANVEFARQVSKYLSLPLSDAGVKRFSDGEISVQIDESVRGKDVFIIQSTC
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCC
APTNDNLMELLILTDALRRSSANSITAIIPYFGYARQDRKANPRVPITAKLVANLIQAAG
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
IDRVATIDLHAGQIQGFFDIPVDNLYGSIVFNDYIKAKHFKNAIIGSPDIGGVARARSVA
CCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
KHLGLDIVIVDKRREKANESEVMNIIGDVKDKEVILVDDIIDTAGTIVKAAEALKEKGAK
HHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHH
SVMACCTHAVLSGKAYERIASGVLDELVVTDTIPLKEQLPNIKVLSVTPVFAEVIRRVYH
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECHHHHHHHHHHHC
NESVNSLFI
CCCCCCCCC
>Mature Secondary Structure
MRGYKIFSGSANVEFARQVSKYLSLPLSDAGVKRFSDGEISVQIDESVRGKDVFIIQSTC
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCC
APTNDNLMELLILTDALRRSSANSITAIIPYFGYARQDRKANPRVPITAKLVANLIQAAG
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
IDRVATIDLHAGQIQGFFDIPVDNLYGSIVFNDYIKAKHFKNAIIGSPDIGGVARARSVA
CCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
KHLGLDIVIVDKRREKANESEVMNIIGDVKDKEVILVDDIIDTAGTIVKAAEALKEKGAK
HHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHH
SVMACCTHAVLSGKAYERIASGVLDELVVTDTIPLKEQLPNIKVLSVTPVFAEVIRRVYH
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECHHHHHHHHHHHC
NESVNSLFI
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204