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The map label for this gene is yibK [C]

Identifier: 57237733

GI number: 57237733

Start: 922011

End: 922478

Strand: Reverse

Name: yibK [C]

Synonym: CJE0983

Alternate gene names: 57237733

Gene position: 922478-922011 (Counterclockwise)

Preceding gene: 57237734

Following gene: 57237732

Centisome position: 51.89

GC content: 29.49

Gene sequence:

>468_bases
ATGTTTAATATAGTTTTAGTTCACCCTAGAATACCGCAAAATACAGGTAGTATAGGTAGAATGTGCTTTAATGCTGGTTT
TAAACTTCATATTGTTAAACCAACTGTTTTTGATATTTCACAAAAGGCTGTTAGAAGGGCAGGACTTGATTACTGGGATA
AATTAGAGCCTATTATTTGGGAGAATCTTGAAGAATTTTTGAATGAAAATATGATTTATAAAAATCGCTTTTTTTTCGCA
ACAACTAAGAGTCAAAAAGCTTATTTTGATGCAGAATTTCAAAAAAATGATTTCTTATTCTTTGGAAGTGAGAGTTATGG
TTTGCCTATGGAATTAATGCAATTAAACTGGGAAAATGCTATAACTATACCTATGAAATCTTATGGAAGAAGTTTAAATT
TAGCCACAAGCGTTGGTATAATCTCTTATGAAGCTTTAAGACAAAATTTTAGAAATTTTGCTTCTTAA

Upstream 100 bases:

>100_bases
TGTTTAAAGATGCTGATATTTGGGCTGATTTTTTTCATACTATTAACTATGAAATTTTAGTAAAATTAAATCCTAATATT
CAAAGAGTTCTAGTATAAAT

Downstream 100 bases:

>100_bases
AAGTTTCAAATTTACACAATAGCTTAAAACAAATAAAATCATAAAGTCCTTTTTATAAAAATTATGTTAAAGTAAAATTT
AGATTATTTTTTGAGGAGTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMIYKNRFFFA
TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQNFRNFAS

Sequences:

>Translated_155_residues
MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMIYKNRFFFA
TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQNFRNFAS
>Mature_155_residues
MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIWENLEEFLNENMIYKNRFFFA
TTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENAITIPMKSYGRSLNLATSVGIISYEALRQNFRNFAS

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=150, Percent_Identity=43.3333333333333, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18101; Mature: 18101

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIW
CEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHCCHHHH
ENLEEFLNENMIYKNRFFFATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENA
HHHHHHHCCCEEEECEEEEEECCCCCEEEECCCCCCCEEEECCCCCCCCHHHHHCCCCCE
ITIPMKSYGRSLNLATSVGIISYEALRQNFRNFAS
EEEEHHHCCCEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFNIVLVHPRIPQNTGSIGRMCFNAGFKLHIVKPTVFDISQKAVRRAGLDYWDKLEPIIW
CEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHCCHHHH
ENLEEFLNENMIYKNRFFFATTKSQKAYFDAEFQKNDFLFFGSESYGLPMELMQLNWENA
HHHHHHHCCCEEEECEEEEEECCCCCEEEECCCCCCCEEEECCCCCCCCHHHHHCCCCCE
ITIPMKSYGRSLNLATSVGIISYEALRQNFRNFAS
EEEEHHHCCCEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]