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The map label for this gene is gltX [H]

Identifier: 57237686

GI number: 57237686

Start: 869783

End: 871078

Strand: Reverse

Name: gltX [H]

Synonym: CJE0932

Alternate gene names: 57237686

Gene position: 871078-869783 (Counterclockwise)

Preceding gene: 57237689

Following gene: 57237685

Centisome position: 49.0

GC content: 30.71

Gene sequence:

>1296_bases
ATGTACCGTTTTGCACCTTCTCCCACAGGTGATATGCACATAGGAAATTTACGCGCAGCAATTTTTAATTATATCTGTGC
AAGACAAAAAAATATGGATTTTATCTTACGTATTGAAGATACTGATAAGGCTAGAAATATAGCAGGAAAAGAAGAAGAAA
TCAAAGAAATTTTAAATCTTTTTAGTATTTCATGGCAGCATTACTATATACAAAGTGAAAATTTAAAATTCCATCGCCAA
ATGGCTTTAAAACTTGTAAGCGAAAAAAAAGCTTTTGCTTGTTTTTGCACCGAAGAAGAACTTGAAGCAAAAAAAGAGCT
GGCTAAAAAACAAGGTAAAGCTTATCGCTACGATGGCACTTGTGAAAAACTAGCCGATATTGATGTTTTAGAATGTGAAA
AACCCTTTGTTATCCGTCTTAAAAAACCTACTCATACTATGAAATTTACTGATTTTATCAAAGGCGAGCTTAGTTTTGAG
CCTGAAAACATAGACTCTTTTGTGATTATGAGAACGGATAAAACTCCAACTTATAATTTTGCTTGTGCGGTTGATGATAT
GCTTGAAAATGTCACTTGTATTATACGCGGAGAAGATCATGTTTCAAACACTCCAAAACAAGAACATATCCGCGCAAGCT
TAGGTTATAATAAAGCCATGACTTATGCACACTTGCCTATCATTTTAAACGAAGAAGGTGTTAAAATGAGTAAAAGAGAA
GCACATTCTTCTGTAAAATGGCTACTTGAAAGTGGAATTTTACCTAGCGCAATCGCAAATTATCTGATCATGCTAGGCAA
TAAAACTCCTTGTGAAATTTTTACCTTAGAAAAGGCTATAAAATGGTTTGATATTTCTAAAGTTTCAAAAGCTCCCGCAA
GATTTGATCTTAAAAAACTTTTGCAAATTAATCGCGAACATATCAAAATGATAAAAGATGATGAGCTTAATAAAATTTTA
GATTTAAACAAAGATCTTGCACCACTTGCTAAATTTTACACTCAAGAAGCAAGTACTATAAAAGAACTTAAAGAAAAAAT
GCAAGCTATTTTTAACGCAAAAAATTTTGGCGAGTTTGAAACAGAATGTAAAATTTTAAAAGAACTCTTAAAAGATATTG
AACTTTTTGAAAATTATGAAGATTTTAAAAATGAACTTTTAAATAAAAGCAACTTAAAAGGTAAAAAATTTTTCATGCCT
TTAAGGATAATTTTAACAGGAAATATCCACGGACCTGAGCTTAGTGATCTTTATCCTTATATTAAAAATTTTATTCACGA
ATTAGCAAGGATTTAA

Upstream 100 bases:

>100_bases
AATTAAAAATATCATAAAAGTATTATAACCAAGTAAAATTAAATATCCGTATAAATTTTTCTGTTATAATAAGCTTTTAA
ATAATTTTAAAGGCTATAAG

Downstream 100 bases:

>100_bases
CTATGGTTATAGACTCTTTTATTATTTCTATTTTTCAAGTACTTCAAATTGTCATTAACATTTATACTTGGATTATTATC
ATCACAGCACTTTTAAGCTG

Product: glutamylglutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MYRFAPSPTGDMHIGNLRAAIFNYICARQKNMDFILRIEDTDKARNIAGKEEEIKEILNLFSISWQHYYIQSENLKFHRQ
MALKLVSEKKAFACFCTEEELEAKKELAKKQGKAYRYDGTCEKLADIDVLECEKPFVIRLKKPTHTMKFTDFIKGELSFE
PENIDSFVIMRTDKTPTYNFACAVDDMLENVTCIIRGEDHVSNTPKQEHIRASLGYNKAMTYAHLPIILNEEGVKMSKRE
AHSSVKWLLESGILPSAIANYLIMLGNKTPCEIFTLEKAIKWFDISKVSKAPARFDLKKLLQINREHIKMIKDDELNKIL
DLNKDLAPLAKFYTQEASTIKELKEKMQAIFNAKNFGEFETECKILKELLKDIELFENYEDFKNELLNKSNLKGKKFFMP
LRIILTGNIHGPELSDLYPYIKNFIHELARI

Sequences:

>Translated_431_residues
MYRFAPSPTGDMHIGNLRAAIFNYICARQKNMDFILRIEDTDKARNIAGKEEEIKEILNLFSISWQHYYIQSENLKFHRQ
MALKLVSEKKAFACFCTEEELEAKKELAKKQGKAYRYDGTCEKLADIDVLECEKPFVIRLKKPTHTMKFTDFIKGELSFE
PENIDSFVIMRTDKTPTYNFACAVDDMLENVTCIIRGEDHVSNTPKQEHIRASLGYNKAMTYAHLPIILNEEGVKMSKRE
AHSSVKWLLESGILPSAIANYLIMLGNKTPCEIFTLEKAIKWFDISKVSKAPARFDLKKLLQINREHIKMIKDDELNKIL
DLNKDLAPLAKFYTQEASTIKELKEKMQAIFNAKNFGEFETECKILKELLKDIELFENYEDFKNELLNKSNLKGKKFFMP
LRIILTGNIHGPELSDLYPYIKNFIHELARI
>Mature_431_residues
MYRFAPSPTGDMHIGNLRAAIFNYICARQKNMDFILRIEDTDKARNIAGKEEEIKEILNLFSISWQHYYIQSENLKFHRQ
MALKLVSEKKAFACFCTEEELEAKKELAKKQGKAYRYDGTCEKLADIDVLECEKPFVIRLKKPTHTMKFTDFIKGELSFE
PENIDSFVIMRTDKTPTYNFACAVDDMLENVTCIIRGEDHVSNTPKQEHIRASLGYNKAMTYAHLPIILNEEGVKMSKRE
AHSSVKWLLESGILPSAIANYLIMLGNKTPCEIFTLEKAIKWFDISKVSKAPARFDLKKLLQINREHIKMIKDDELNKIL
DLNKDLAPLAKFYTQEASTIKELKEKMQAIFNAKNFGEFETECKILKELLKDIELFENYEDFKNELLNKSNLKGKKFFMP
LRIILTGNIHGPELSDLYPYIKNFIHELARI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=329, Percent_Identity=31.9148936170213, Blast_Score=172, Evalue=7e-43,
Organism=Escherichia coli, GI1788743, Length=445, Percent_Identity=32.3595505617978, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI226510925, Length=239, Percent_Identity=30.9623430962343, Blast_Score=122, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=469, Percent_Identity=30.2771855010661, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324540, Length=347, Percent_Identity=33.1412103746398, Blast_Score=189, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24665321, Length=462, Percent_Identity=26.8398268398268, Blast_Score=178, Evalue=7e-45,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 50069; Mature: 50069

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRFAPSPTGDMHIGNLRAAIFNYICARQKNMDFILRIEDTDKARNIAGKEEEIKEILNL
CCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCHHHHHHHHHH
FSISWQHYYIQSENLKFHRQMALKLVSEKKAFACFCTEEELEAKKELAKKQGKAYRYDGT
HHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCEECCCC
CEKLADIDVLECEKPFVIRLKKPTHTMKFTDFIKGELSFEPENIDSFVIMRTDKTPTYNF
HHHHCCCCCEECCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEE
ACAVDDMLENVTCIIRGEDHVSNTPKQEHIRASLGYNKAMTYAHLPIILNEEGVKMSKRE
EEEHHHHHCCEEEEEECCHHCCCCCHHHHHHHHCCCCHHHHEEECCEEECCCCCCHHHHH
AHSSVKWLLESGILPSAIANYLIMLGNKTPCEIFTLEKAIKWFDISKVSKAPARFDLKKL
HHHHHHHHHHCCCCHHHHHHHHEEECCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHH
LQINREHIKMIKDDELNKILDLNKDLAPLAKFYTQEASTIKELKEKMQAIFNAKNFGEFE
HHHCHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
TECKILKELLKDIELFENYEDFKNELLNKSNLKGKKFFMPLRIILTGNIHGPELSDLYPY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHH
IKNFIHELARI
HHHHHHHHHCC
>Mature Secondary Structure
MYRFAPSPTGDMHIGNLRAAIFNYICARQKNMDFILRIEDTDKARNIAGKEEEIKEILNL
CCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCHHHHHHHHHH
FSISWQHYYIQSENLKFHRQMALKLVSEKKAFACFCTEEELEAKKELAKKQGKAYRYDGT
HHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCEECCCC
CEKLADIDVLECEKPFVIRLKKPTHTMKFTDFIKGELSFEPENIDSFVIMRTDKTPTYNF
HHHHCCCCCEECCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEE
ACAVDDMLENVTCIIRGEDHVSNTPKQEHIRASLGYNKAMTYAHLPIILNEEGVKMSKRE
EEEHHHHHCCEEEEEECCHHCCCCCHHHHHHHHCCCCHHHHEEECCEEECCCCCCHHHHH
AHSSVKWLLESGILPSAIANYLIMLGNKTPCEIFTLEKAIKWFDISKVSKAPARFDLKKL
HHHHHHHHHHCCCCHHHHHHHHEEECCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHH
LQINREHIKMIKDDELNKILDLNKDLAPLAKFYTQEASTIKELKEKMQAIFNAKNFGEFE
HHHCHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
TECKILKELLKDIELFENYEDFKNELLNKSNLKGKKFFMPLRIILTGNIHGPELSDLYPY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHH
IKNFIHELARI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA