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The map label for this gene is trmA [H]

Identifier: 57237672

GI number: 57237672

Start: 855144

End: 856217

Strand: Reverse

Name: trmA [H]

Synonym: CJE0918

Alternate gene names: 57237672

Gene position: 856217-855144 (Counterclockwise)

Preceding gene: 57237673

Following gene: 57237670

Centisome position: 48.16

GC content: 26.26

Gene sequence:

>1074_bases
ATGAGTCTTGAAAATTTTGGAAATTTTCTTACTTTAGATGAAAAACATTCTTTCATAAAAAAATATTTTAAAGAATTTTA
TACAAAGGATTTTAAACTTTTTGCTTCAAAAGATAAGCATTATCGTACAAGAGCTGAACTTTCATTTTATCATGAAAACG
ATACGCTTTTTTATGCGATGTTTGATCCAAAAAACAAAAAAAAGTACATTATAAAATATTTAGATTTTGCTGATGAAAAA
ATTTGTACTTTTATGCCAAAACTTCTAGAATACTTGCGTCAAGATAATAAATTAAAAGAAAAACTTTTTGGCGTGGAGTT
TTTAACCACAAAGCAAGAACTTAGCGTAACTTTACTCTATCATAAAAATATAGAAGATATAAAGTCAAATTTAGAAAATT
TAAGCAATATTCTTCATATCAACCTTATAGCAAGAAGTAAAGGCAAAAAGCTTATTTTTAAAACAGAAAATCTAAGACAA
ACTTTAAATATTCAAGGTAGAAAAATATTTTATGAATTTAACAACGATTGTTTTATTCAACCTAACACTACTATAAATGA
AAAAATGATTACTTGGGTGTGTGAAATTTTAAACACTCAAAAAAGAATGGATTTACTTGAGCTTTACTGTGGATATGGAA
ATTTTACTTTAGCTTTAGCACCATTCTTTTTTAAAGTTTTAGCCACTGAAATTTCAAAAAGCAATATTAATTTTGCTTTA
AAAAATTGCGAGCTTAACAATACAACAAATATCCACTTTGCAAGACTTTCAAGCGAAGAATTAAGTCTTGCTATCAAAAA
AGAAAGAGAATTTTTTCGTTTAAAAGATATTCGATTAGATGATTTTAATTTCTCTCATGTTTTAGTTGATCCACCCCGTG
CAGGACTTGATAAAAGCGTAATTGACTTAATAAAAAAATATGAAAATATTATTTATATTTCTTGCAATCCTATCACTTTA
AAAGAAAATTTAAAAGAATTAAGCCTAACACATAGAGTGGAAGAGTTTGCTCTGTTTGATCAATTTGTCAATACTCCACA
TCTTGAATGCGGAGTATTTCTTAGCAAGGTATAA

Upstream 100 bases:

>100_bases
GATATATTCCTGTATCTTTAGGACTTAGCGTTTGGTTTGTCTTGCCTTTAAATTTCATACTTATTGCACTTTTACTAAGA
CTCATCGGAAAGAAAGTTCC

Downstream 100 bases:

>100_bases
AAAAATAATTCATTAATTTAGCCAGTATTACTATTGCAATACAAAGCACAAATACAAAAGGGTGGATAAAAGCACCGAGT
GGATTTTTCTTCTTTAATAT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFASKDKHYRTRAELSFYHENDTLFYAMFDPKNKKKYIIKYLDFADEK
ICTFMPKLLEYLRQDNKLKEKLFGVEFLTTKQELSVTLLYHKNIEDIKSNLENLSNILHINLIARSKGKKLIFKTENLRQ
TLNIQGRKIFYEFNNDCFIQPNTTINEKMITWVCEILNTQKRMDLLELYCGYGNFTLALAPFFFKVLATEISKSNINFAL
KNCELNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSVIDLIKKYENIIYISCNPITL
KENLKELSLTHRVEEFALFDQFVNTPHLECGVFLSKV

Sequences:

>Translated_357_residues
MSLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFASKDKHYRTRAELSFYHENDTLFYAMFDPKNKKKYIIKYLDFADEK
ICTFMPKLLEYLRQDNKLKEKLFGVEFLTTKQELSVTLLYHKNIEDIKSNLENLSNILHINLIARSKGKKLIFKTENLRQ
TLNIQGRKIFYEFNNDCFIQPNTTINEKMITWVCEILNTQKRMDLLELYCGYGNFTLALAPFFFKVLATEISKSNINFAL
KNCELNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSVIDLIKKYENIIYISCNPITL
KENLKELSLTHRVEEFALFDQFVNTPHLECGVFLSKV
>Mature_356_residues
SLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFASKDKHYRTRAELSFYHENDTLFYAMFDPKNKKKYIIKYLDFADEKI
CTFMPKLLEYLRQDNKLKEKLFGVEFLTTKQELSVTLLYHKNIEDIKSNLENLSNILHINLIARSKGKKLIFKTENLRQT
LNIQGRKIFYEFNNDCFIQPNTTINEKMITWVCEILNTQKRMDLLELYCGYGNFTLALAPFFFKVLATEISKSNINFALK
NCELNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSVIDLIKKYENIIYISCNPITLK
ENLKELSLTHRVEEFALFDQFVNTPHLECGVFLSKV

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=351, Percent_Identity=37.6068376068376, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1789148, Length=191, Percent_Identity=32.9842931937173, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787083, Length=267, Percent_Identity=24.3445692883895, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24666579, Length=201, Percent_Identity=29.3532338308458, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 42303; Mature: 42171

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFASKDKHYRTRAELSFYHENDTLFYAM
CCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEECCCEEEEEE
FDPKNKKKYIIKYLDFADEKICTFMPKLLEYLRQDNKLKEKLFGVEFLTTKQELSVTLLY
ECCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECHHHCEEEEEE
HKNIEDIKSNLENLSNILHINLIARSKGKKLIFKTENLRQTLNIQGRKIFYEFNNDCFIQ
ECCHHHHHHHHHHHHHEEEEEEEEECCCCEEEEECCCHHHHHCCCCEEEEEEECCEEEEC
PNTTINEKMITWVCEILNTQKRMDLLELYCGYGNFTLALAPFFFKVLATEISKSNINFAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
KNCELNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSV
EECCCCCCCEEEEEEECCHHHHEEEHHHHHHEEHCCCEECCCCCCEEEECCCCCCCCHHH
IDLIKKYENIIYISCNPITLKENLKELSLTHRVEEFALFDQFVNTPHLECGVFLSKV
HHHHHHHCCEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECC
>Mature Secondary Structure 
SLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFASKDKHYRTRAELSFYHENDTLFYAM
CCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEECCCEEEEEE
FDPKNKKKYIIKYLDFADEKICTFMPKLLEYLRQDNKLKEKLFGVEFLTTKQELSVTLLY
ECCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECHHHCEEEEEE
HKNIEDIKSNLENLSNILHINLIARSKGKKLIFKTENLRQTLNIQGRKIFYEFNNDCFIQ
ECCHHHHHHHHHHHHHEEEEEEEEECCCCEEEEECCCHHHHHCCCCEEEEEEECCEEEEC
PNTTINEKMITWVCEILNTQKRMDLLELYCGYGNFTLALAPFFFKVLATEISKSNINFAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
KNCELNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSV
EECCCCCCCEEEEEEECCHHHHEEEHHHHHHEEHCCCEECCCCCCEEEECCCCCCCCHHH
IDLIKKYENIIYISCNPITLKENLKELSLTHRVEEFALFDQFVNTPHLECGVFLSKV
HHHHHHHCCEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA