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The map label for this gene is ddl [H]

Identifier: 57237643

GI number: 57237643

Start: 826721

End: 827761

Strand: Reverse

Name: ddl [H]

Synonym: CJE0889

Alternate gene names: 57237643

Gene position: 827761-826721 (Counterclockwise)

Preceding gene: 57237644

Following gene: 57237642

Centisome position: 46.56

GC content: 29.2

Gene sequence:

>1041_bases
ATGAAATTTGCAATACTTTTTGGCGGAAATTCTTATGAACATGAGATCAGTATAGTAAGTGCTGTAGTACTTAAAAAAGT
TATTAATCAAAATTTAGAATTTATTTTTTGTGATGAAGAAAGAAGATTTTATCATATCCCAAGTGAAAAAATGAATTCTA
AAACTTTTAGCACCAAAGCTTATAAAAAAGAAAAAGAACTTTTCATCAAACAAGGAGGTTTTTTTAGCAAAGGCTTTTTA
AAAGAAAATAAACTAGAGTGCGAGTGCGTGATCAATCTTATCCATGGTAGAGATGGAGAAGATGGAAAAATTGCAGCTTT
ATTTGAGTTTTATTCTATTAAATTTATAGGACCTAGGCTTGAAGCCAGCGTATTATCTTTCAACAAAGAACTTACCAAAC
TTTATGCCAAAAGCGTTGGAGTAAAAACACTTGATTATACCATGGTAAGAAAAGGGCAAAACTCTAAAGAAAAGCTTAGT
TTTCCTTGCATTATCAAACCTGCAAGACTTGGAAGTAGCATAGGTATTAGTATAGTAAAAGATGAAAAAGATTTAGAATA
CGCCAAAGATGTAGGTTTTGAATTTGATAATGATCTTGTAGTGGAAGAATTTAAAAACAATATCAAAGAATACAATCTTG
CAGGATGTATGATCAATGATGAGTTTGTTTTTTCCATCATAGAAGAGCCAAAGAAAAAAGAATTTTTAGACTTTGAACAA
AAATATCTTAGCTTTTCAGGACACAATGAGCTTATAGAAGCAAATTTAAGCGAAGAATTAAAAGAAAAACTCAAAGATAG
TTTTAAAAAGATCTACAATCCTTTGTTTAAAGGTGCTTTAATACGCTGCGATTTTTTTATACTGGATAATGAAATATATT
TAAATGAAATCAACCCAAATCCTGGTTCTTTGGCTAATTATCTTTTTAAAGATTTTAGTACTACTTTAAACGCTTTAGCT
GATCAAATTTCACTTGAAAAAATGATAAAAATCAGCTACAACTTCCTTCACTCTATCAACGGACAAAAAGGAAAATTATA
A

Upstream 100 bases:

>100_bases
TAAACAAGAAAAAATCATAAGTGTTTTAGCAAGTGCTCAAGCAACAGGCACAAGCGAACTCATCAAAGAAGCTCTTAAAA
AACTAGGATAAAGGAATAAA

Downstream 100 bases:

>100_bases
GCTTTTAAGTCAAAATACGCTAAATTATACATAAAATTTAGCGTATTTTAGGAAGAAAAATGCTCTCTTTTAAACAAGAT
GAAATTTACACTGCCACCGA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKFAILFGGNSYEHEISIVSAVVLKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKAYKKEKELFIKQGGFFSKGFL
KENKLECECVINLIHGRDGEDGKIAALFEFYSIKFIGPRLEASVLSFNKELTKLYAKSVGVKTLDYTMVRKGQNSKEKLS
FPCIIKPARLGSSIGISIVKDEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCMINDEFVFSIIEEPKKKEFLDFEQ
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEIYLNEINPNPGSLANYLFKDFSTTLNALA
DQISLEKMIKISYNFLHSINGQKGKL

Sequences:

>Translated_346_residues
MKFAILFGGNSYEHEISIVSAVVLKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKAYKKEKELFIKQGGFFSKGFL
KENKLECECVINLIHGRDGEDGKIAALFEFYSIKFIGPRLEASVLSFNKELTKLYAKSVGVKTLDYTMVRKGQNSKEKLS
FPCIIKPARLGSSIGISIVKDEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCMINDEFVFSIIEEPKKKEFLDFEQ
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEIYLNEINPNPGSLANYLFKDFSTTLNALA
DQISLEKMIKISYNFLHSINGQKGKL
>Mature_346_residues
MKFAILFGGNSYEHEISIVSAVVLKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKAYKKEKELFIKQGGFFSKGFL
KENKLECECVINLIHGRDGEDGKIAALFEFYSIKFIGPRLEASVLSFNKELTKLYAKSVGVKTLDYTMVRKGQNSKEKLS
FPCIIKPARLGSSIGISIVKDEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCMINDEFVFSIIEEPKKKEFLDFEQ
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEIYLNEINPNPGSLANYLFKDFSTTLNALA
DQISLEKMIKISYNFLHSINGQKGKL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=330, Percent_Identity=25.1515151515152, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786280, Length=319, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39800; Mature: 39800

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAILFGGNSYEHEISIVSAVVLKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHCCCCHHHHH
YKKEKELFIKQGGFFSKGFLKENKLECECVINLIHGRDGEDGKIAALFEFYSIKFIGPRL
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEHHHCCCCCCCCEEEEEEEEEEEEECCCH
EASVLSFNKELTKLYAKSVGVKTLDYTMVRKGQNSKEKLSFPCIIKPARLGSSIGISIVK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCEEEEHHHCCCCCCEEEEE
DEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCMINDEFVFSIIEEPKKKEFLDFEQ
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHHH
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEIYLNEINPN
HHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECEEEEEECCCC
PGSLANYLFKDFSTTLNALADQISLEKMIKISYNFLHSINGQKGKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKFAILFGGNSYEHEISIVSAVVLKKVINQNLEFIFCDEERRFYHIPSEKMNSKTFSTKA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHCCCCHHHHH
YKKEKELFIKQGGFFSKGFLKENKLECECVINLIHGRDGEDGKIAALFEFYSIKFIGPRL
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEHHHCCCCCCCCEEEEEEEEEEEEECCCH
EASVLSFNKELTKLYAKSVGVKTLDYTMVRKGQNSKEKLSFPCIIKPARLGSSIGISIVK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCEEEEHHHCCCCCCEEEEE
DEKDLEYAKDVGFEFDNDLVVEEFKNNIKEYNLAGCMINDEFVFSIIEEPKKKEFLDFEQ
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHHH
KYLSFSGHNELIEANLSEELKEKLKDSFKKIYNPLFKGALIRCDFFILDNEIYLNEINPN
HHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECEEEEEECCCC
PGSLANYLFKDFSTTLNALADQISLEKMIKISYNFLHSINGQKGKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA