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The map label for this gene is murF [H]

Identifier: 57237640

GI number: 57237640

Start: 824297

End: 825724

Strand: Reverse

Name: murF [H]

Synonym: CJE0886

Alternate gene names: 57237640

Gene position: 825724-824297 (Counterclockwise)

Preceding gene: 57237641

Following gene: 57237636

Centisome position: 46.45

GC content: 28.64

Gene sequence:

>1428_bases
ATGCTTATCAATACTCTTTATTTTATCAATTCTTTATTTTTTAATTTTTGCATAGCTTTTTATCTTATGAGTGCTTTACA
ATGGTATTCTTATAAATTTAAACGCGTGTTTTTTCATTATCATAAACCTTTATGGCATTTGTATTTTTTATTTATCCCTT
ATTTTTTATTCTTAGCTTTTCCTTTATACTCTTTGGCATATTTTGCATTGATCCATACTCCGATTTTGTATTTTTGGAAT
AAAGGCATAGATAAAAAGCTTGTTTTTACAAGTAAGGTTAAATGGTTTTTTGTTTTTGTTTTTGTTTATAATGCTATTTT
TACCATCCTTGCTTTGCGTTTTTCTTTTCTTTTTAATCTTTTTAGTCTTCCTTTTGCTTTATTGAGTCTTAAAATTTATG
AGTTTTTTACAAATTTATATTATAAAAAACAAGCCAAAGCCAAACTAAGAGCCAATGAAAATTTAAAAATCATACTCATT
ACCGCAAGTTTTGGAAAAACAAGTATTAAAAATTTTTTATACGAACTTTTAAAAGATGACTTTAAAAGCTACAAAACACC
ACGATCTGTCAATACCTTGCTAGGTATAGTTGCTGATATCAATAACAATTTAAGCCAAGATACTCAAATTTACATAGCTG
AAGCAGGTGCTAGACTTAAAGGCGATATTGATGAAATCACTCGTTTTTTACAACCTCACATCAGCATAGTAGGAGAGATA
GGCAATGTACATTTAGAGTATTTTAAAAGTGTTGAAAATATCCTCTCAACCAAACTTGAAGCCTTAAATTCCAAAAGACT
AGAAAAGGCTTTTTTACACTCTAGCACCCAAAAAGAAGGAGATAAACTCATCAGCCTTTATGATGAGAAATTAAGTCTTA
TTCATTCAAGTCTTGAAGGTTTAGAATTTAAAGTCGATATAGAAAATAAATCCTATGATTTTAAAAGTCAAATTTTAGGG
GATTTTAATGCGCAAAATCTTTGTGTTTGTATACTTTGTGCTCATTATCTTGGCATAAAACTAGAAAAAATTCAAAAGCA
AGTTTTAAATATAAATTCCGTAGAACATCGCTTGCAAGTGCTTTCAAGAGAGCCTAAATTTATCATAGATGATGGTTTTA
ATGGAAATTTTAAAGGTATGAGCACAAGCTATAAACTTTGTAAAAGCTATAAAGGACGCAAAGTTTTAGTAAGTCCTGGT
ATAGTAGAAGTAAGCGAAGAAGAAAATATAAAACTTGCTAAAATCATCAATGAGTGTTTTGATCTTGCTATCATTAGTGC
ACAAATCAATGCTGAAATTTTCAAAAAAGAGTTAAAAATAAAAACCATTATACTTAAAGAAAAGAGTCAGCTAGTTCAAA
CTCTAGCTAAAGAAACAAAACATGGAGATTTGATACTCTTTTCAAACGATGCGCCTAGCTTTATGTGA

Upstream 100 bases:

>100_bases
TCAACTTTTAAAAAATAGCACTTTTTATCCTTTAGAGGGGGATCATTTTTTCTTTTTAAAATATTCTGCTTTCATAGCTC
AAAAAATTAAGGAAATTTAA

Downstream 100 bases:

>100_bases
TTTGCATAAGTATTTGCAAATGTCATCATTTTTAATTTGACTTCGTGCAAATACTTCCTAAACCCTAGTCTGCCCTATAA
GATTATTAAGTCTATCCATT

Product: Mur ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLINTLYFINSLFFNFCIAFYLMSALQWYSYKFKRVFFHYHKPLWHLYFLFIPYFLFLAFPLYSLAYFALIHTPILYFWN
KGIDKKLVFTSKVKWFFVFVFVYNAIFTILALRFSFLFNLFSLPFALLSLKIYEFFTNLYYKKQAKAKLRANENLKIILI
TASFGKTSIKNFLYELLKDDFKSYKTPRSVNTLLGIVADINNNLSQDTQIYIAEAGARLKGDIDEITRFLQPHISIVGEI
GNVHLEYFKSVENILSTKLEALNSKRLEKAFLHSSTQKEGDKLISLYDEKLSLIHSSLEGLEFKVDIENKSYDFKSQILG
DFNAQNLCVCILCAHYLGIKLEKIQKQVLNINSVEHRLQVLSREPKFIIDDGFNGNFKGMSTSYKLCKSYKGRKVLVSPG
IVEVSEEENIKLAKIINECFDLAIISAQINAEIFKKELKIKTIILKEKSQLVQTLAKETKHGDLILFSNDAPSFM

Sequences:

>Translated_475_residues
MLINTLYFINSLFFNFCIAFYLMSALQWYSYKFKRVFFHYHKPLWHLYFLFIPYFLFLAFPLYSLAYFALIHTPILYFWN
KGIDKKLVFTSKVKWFFVFVFVYNAIFTILALRFSFLFNLFSLPFALLSLKIYEFFTNLYYKKQAKAKLRANENLKIILI
TASFGKTSIKNFLYELLKDDFKSYKTPRSVNTLLGIVADINNNLSQDTQIYIAEAGARLKGDIDEITRFLQPHISIVGEI
GNVHLEYFKSVENILSTKLEALNSKRLEKAFLHSSTQKEGDKLISLYDEKLSLIHSSLEGLEFKVDIENKSYDFKSQILG
DFNAQNLCVCILCAHYLGIKLEKIQKQVLNINSVEHRLQVLSREPKFIIDDGFNGNFKGMSTSYKLCKSYKGRKVLVSPG
IVEVSEEENIKLAKIINECFDLAIISAQINAEIFKKELKIKTIILKEKSQLVQTLAKETKHGDLILFSNDAPSFM
>Mature_475_residues
MLINTLYFINSLFFNFCIAFYLMSALQWYSYKFKRVFFHYHKPLWHLYFLFIPYFLFLAFPLYSLAYFALIHTPILYFWN
KGIDKKLVFTSKVKWFFVFVFVYNAIFTILALRFSFLFNLFSLPFALLSLKIYEFFTNLYYKKQAKAKLRANENLKIILI
TASFGKTSIKNFLYELLKDDFKSYKTPRSVNTLLGIVADINNNLSQDTQIYIAEAGARLKGDIDEITRFLQPHISIVGEI
GNVHLEYFKSVENILSTKLEALNSKRLEKAFLHSSTQKEGDKLISLYDEKLSLIHSSLEGLEFKVDIENKSYDFKSQILG
DFNAQNLCVCILCAHYLGIKLEKIQKQVLNINSVEHRLQVLSREPKFIIDDGFNGNFKGMSTSYKLCKSYKGRKVLVSPG
IVEVSEEENIKLAKIINECFDLAIISAQINAEIFKKELKIKTIILKEKSQLVQTLAKETKHGDLILFSNDAPSFM

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 55203; Mature: 55203

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLINTLYFINSLFFNFCIAFYLMSALQWYSYKFKRVFFHYHKPLWHLYFLFIPYFLFLAF
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLYSLAYFALIHTPILYFWNKGIDKKLVFTSKVKWFFVFVFVYNAIFTILALRFSFLFNL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLPFALLSLKIYEFFTNLYYKKQAKAKLRANENLKIILITASFGKTSIKNFLYELLKDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
FKSYKTPRSVNTLLGIVADINNNLSQDTQIYIAEAGARLKGDIDEITRFLQPHISIVGEI
HHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
GNVHLEYFKSVENILSTKLEALNSKRLEKAFLHSSTQKEGDKLISLYDEKLSLIHSSLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
LEFKVDIENKSYDFKSQILGDFNAQNLCVCILCAHYLGIKLEKIQKQVLNINSVEHRLQV
CEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHH
LSREPKFIIDDGFNGNFKGMSTSYKLCKSYKGRKVLVSPGIVEVSEEENIKLAKIINECF
HCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCEEEECCCEEEECCCCCCHHHHHHHHHH
DLAIISAQINAEIFKKELKIKTIILKEKSQLVQTLAKETKHGDLILFSNDAPSFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MLINTLYFINSLFFNFCIAFYLMSALQWYSYKFKRVFFHYHKPLWHLYFLFIPYFLFLAF
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLYSLAYFALIHTPILYFWNKGIDKKLVFTSKVKWFFVFVFVYNAIFTILALRFSFLFNL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLPFALLSLKIYEFFTNLYYKKQAKAKLRANENLKIILITASFGKTSIKNFLYELLKDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
FKSYKTPRSVNTLLGIVADINNNLSQDTQIYIAEAGARLKGDIDEITRFLQPHISIVGEI
HHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
GNVHLEYFKSVENILSTKLEALNSKRLEKAFLHSSTQKEGDKLISLYDEKLSLIHSSLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
LEFKVDIENKSYDFKSQILGDFNAQNLCVCILCAHYLGIKLEKIQKQVLNINSVEHRLQV
CEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHH
LSREPKFIIDDGFNGNFKGMSTSYKLCKSYKGRKVLVSPGIVEVSEEENIKLAKIINECF
HCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCEEEECCCEEEECCCCCCHHHHHHHHHH
DLAIISAQINAEIFKKELKIKTIILKEKSQLVQTLAKETKHGDLILFSNDAPSFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]