Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 57237487

GI number: 57237487

Start: 438113

End: 439321

Strand: Reverse

Name: murD [H]

Synonym: CJE0482

Alternate gene names: 57237487

Gene position: 439321-438113 (Counterclockwise)

Preceding gene: 57237488

Following gene: 57237484

Centisome position: 24.71

GC content: 31.51

Gene sequence:

>1209_bases
ATGAAAATTTCACTTTTTGGATATGGAAAAACCACACGCGCTATTGCTGAAAATTTGGTAGATAAATTTGGGTCTTTTGA
TATCTATGATGATCATTTTGTAGAAACAAAAAAAGATACACTAGGAAATTTACTTTTAAATCCTAATGATTTTGATAATA
ATTTAAGCGATATTGAAATTCCTAGTCCAGGTTTTCCGCCAAAGCACAAACTCATTCAAAAAGCAAAAAATTTACAAAGT
GAATATGACTTTTTTTATGATATCATGCCAAAATCCGTTTGGATAAGCGGAACCAATGGCAAAACCACTACCACTCAAAT
GGCTACACATTTATTATCACATATTGGTGCTGTTATGGGTGGAAATGTTGGAACTCCTTTAGCAGAGCTTAATCCTTATG
AAAAACTTTGGATACTTGAAACTTCATCTTTTACCCTACACTACACTCATAAAGCTAAACCTGAAATTTATGCACTTTTA
CCCATCAGTCCTGATCATCTTTCTTGGCATGGAAGTTTTGATAATTATGTTCAAGATAAGCTAAGCGTTTTAAAAAGAAT
GAATGAATGTGATGTAACAATACTGCCTAAAATTTATGCAAATACTCCAACAAAAGCTCATATAATAAGCTATAAAGATG
AAAAAGATCTGGCTGCTAAATTTAGCATTGATATGGAAAAAATTTCCTTTAAAAGTCCTTTTTTACTCGATGCTATCATG
GCTTTAGCCATAGAAAAAATTCTACTTGATACTTTAAGCTATGAGCTTTTAAATAGCTTTGTAATGGAAAAAAATAAACT
TGAAGAGATTAAAGACAGTCAAAATAGACTTTGGGTAAATGACACTAAAGCCACAAATGAAAGCGCGGTAATGGCTGCAC
TTAATCGCTATAAAGATAAAAAAATCCATCTTATCATAGGGGGAGATGATAAGGGTGTAGATCTTAGCAATTTATTTGAT
TTTATGAAAGGATTTGATATAGAACTCTATGCTATAGGTATTAGTACTGAAAAAATGCTTGATTATGCAAAAAAAGCGAA
TTTGAAAGCCTATAAATGTGAAGTTTTATCTAAGGCTGTAAATGAAATTTCAAATCACTTAAGAGTCAATGAAGTGGCTC
TTTTAAGTCCTGCTTGTGCAAGTTTAGATCAGTTCAACTCTTACGCAGAGCGTGGAAAAGTCTTTAAAGAATGTGTGAAT
AAAATTTAA

Upstream 100 bases:

>100_bases
TTGAAAAAGTTGGCTGGGTAGAAAATAAAATTATAGTGCGTTTTTGGATGATAGCCCTACTTTCAAATTTACTTGCACTT
GCATCAATAAAGCTAAGATA

Downstream 100 bases:

>100_bases
TTTATTATACAATCCATACAAATATATTAAACTTTCCAAACCCCTAAAAACCCTAACCAGCAGCAAACTCTTTTTTTAAA
CACGATTCTAAGCACCATCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKISLFGYGKTTRAIAENLVDKFGSFDIYDDHFVETKKDTLGNLLLNPNDFDNNLSDIEIPSPGFPPKHKLIQKAKNLQS
EYDFFYDIMPKSVWISGTNGKTTTTQMATHLLSHIGAVMGGNVGTPLAELNPYEKLWILETSSFTLHYTHKAKPEIYALL
PISPDHLSWHGSFDNYVQDKLSVLKRMNECDVTILPKIYANTPTKAHIISYKDEKDLAAKFSIDMEKISFKSPFLLDAIM
ALAIEKILLDTLSYELLNSFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDKKIHLIIGGDDKGVDLSNLFD
FMKGFDIELYAIGISTEKMLDYAKKANLKAYKCEVLSKAVNEISNHLRVNEVALLSPACASLDQFNSYAERGKVFKECVN
KI

Sequences:

>Translated_402_residues
MKISLFGYGKTTRAIAENLVDKFGSFDIYDDHFVETKKDTLGNLLLNPNDFDNNLSDIEIPSPGFPPKHKLIQKAKNLQS
EYDFFYDIMPKSVWISGTNGKTTTTQMATHLLSHIGAVMGGNVGTPLAELNPYEKLWILETSSFTLHYTHKAKPEIYALL
PISPDHLSWHGSFDNYVQDKLSVLKRMNECDVTILPKIYANTPTKAHIISYKDEKDLAAKFSIDMEKISFKSPFLLDAIM
ALAIEKILLDTLSYELLNSFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDKKIHLIIGGDDKGVDLSNLFD
FMKGFDIELYAIGISTEKMLDYAKKANLKAYKCEVLSKAVNEISNHLRVNEVALLSPACASLDQFNSYAERGKVFKECVN
KI
>Mature_402_residues
MKISLFGYGKTTRAIAENLVDKFGSFDIYDDHFVETKKDTLGNLLLNPNDFDNNLSDIEIPSPGFPPKHKLIQKAKNLQS
EYDFFYDIMPKSVWISGTNGKTTTTQMATHLLSHIGAVMGGNVGTPLAELNPYEKLWILETSSFTLHYTHKAKPEIYALL
PISPDHLSWHGSFDNYVQDKLSVLKRMNECDVTILPKIYANTPTKAHIISYKDEKDLAAKFSIDMEKISFKSPFLLDAIM
ALAIEKILLDTLSYELLNSFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDKKIHLIIGGDDKGVDLSNLFD
FMKGFDIELYAIGISTEKMLDYAKKANLKAYKCEVLSKAVNEISNHLRVNEVALLSPACASLDQFNSYAERGKVFKECVN
KI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=378, Percent_Identity=27.5132275132275, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 45421; Mature: 45421

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLFGYGKTTRAIAENLVDKFGSFDIYDDHFVETKKDTLGNLLLNPNDFDNNLSDIEI
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCEEECCCCCCCCCCCCCC
PSPGFPPKHKLIQKAKNLQSEYDFFYDIMPKSVWISGTNGKTTTTQMATHLLSHIGAVMG
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
GNVGTPLAELNPYEKLWILETSSFTLHYTHKAKPEIYALLPISPDHLSWHGSFDNYVQDK
CCCCCCHHHCCCCCEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHH
LSVLKRMNECDVTILPKIYANTPTKAHIISYKDEKDLAAKFSIDMEKISFKSPFLLDAIM
HHHHHHCCCCCEEEECHHHCCCCCCEEEEEECCCCCHHHHHCCCHHHHCCCCCHHHHHHH
ALAIEKILLDTLSYELLNSFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCC
KIHLIIGGDDKGVDLSNLFDFMKGFDIELYAIGISTEKMLDYAKKANLKAYKCEVLSKAV
EEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHH
NEISNHLRVNEVALLSPACASLDQFNSYAERGKVFKECVNKI
HHHHHHCCHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKISLFGYGKTTRAIAENLVDKFGSFDIYDDHFVETKKDTLGNLLLNPNDFDNNLSDIEI
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCEEECCCCCCCCCCCCCC
PSPGFPPKHKLIQKAKNLQSEYDFFYDIMPKSVWISGTNGKTTTTQMATHLLSHIGAVMG
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
GNVGTPLAELNPYEKLWILETSSFTLHYTHKAKPEIYALLPISPDHLSWHGSFDNYVQDK
CCCCCCHHHCCCCCEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHH
LSVLKRMNECDVTILPKIYANTPTKAHIISYKDEKDLAAKFSIDMEKISFKSPFLLDAIM
HHHHHHCCCCCEEEECHHHCCCCCCEEEEEECCCCCHHHHHCCCHHHHCCCCCHHHHHHH
ALAIEKILLDTLSYELLNSFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCC
KIHLIIGGDDKGVDLSNLFDFMKGFDIELYAIGISTEKMLDYAKKANLKAYKCEVLSKAV
EEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHH
NEISNHLRVNEVALLSPACASLDQFNSYAERGKVFKECVNKI
HHHHHHCCHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA