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The map label for this gene is gppA [H]

Identifier: 57237408

GI number: 57237408

Start: 360031

End: 361491

Strand: Reverse

Name: gppA [H]

Synonym: CJE0402

Alternate gene names: 57237408

Gene position: 361491-360031 (Counterclockwise)

Preceding gene: 57237409

Following gene: 57237399

Centisome position: 20.33

GC content: 29.23

Gene sequence:

>1461_bases
ATGGCAAAAAAAACAGCCGTTGTTGATCTTGGCTCAAACTCCATTAGAATGGTGATTTTTGAAAAAACTTCTAGATATGG
TTTTTATACAACTTGCGAATACAAACGCAAAGTAAGACTTGGAGAAAATGCTTATAATAATGGCAAAATTCTCCAAGAAG
AAGCCATGCAAAGAGCCGAAGATGCCTTAGCTTTTTTCAAACAATGCGCTTTAAAGCATAAGTGCAAAAAAATATTTATA
GTAGGCACATCAGCCTTAAGAGATGCCCCAAATTCAAAAAATTTCATTAAAAGAATCAAAGATAATCTGTCTTTAAATAT
ACGCTGTATAGATGGAAAAAGCGAAAGTTACTTAGGTGGTCTTGCAGCTCTAAATTTATTAAGTCCTTTTAAGGATGGAA
CCACTTTAGACATAGGTGGTGGTTCTAGTGAATTGTGTTTAATAAAAAATAATAGAATTATTTCTTGCATATCTCTTGAT
ATTGGCACTGTTAGGCTAAAAGAATTGTTCTATGATACTGGAAAAATGGATTCTTTAGAAGAATTTATAAAACCAATTTT
AGAACAAATTCCTAAAGAATTTTGCAATCAAAACCTTATTGCAATAGGTGGAAGCTTAAGAGCAATATCTAATTCCATCA
TGCAAAAAAATTCCTATCCACTGAAAAATTTACATGATTTTCGCTATATGCTAGATGAAGAAAAAGGGCATATTTTAAAA
ATTTTTAATTCAAACTTAGACTCTTTAATCAATTTTGGCATAAAAAAGGATCGTTTTGATACCATTAAAGAGGGTATATT
TATTTTTTTAAAGATTGCGGAAAAAATAAAAGCCAAACAAGTTATCACGAGTGGGGTTGGAATCAGAGAAGGAGTTTATT
TGCAAGATTTACTTCGTCCTAAAATCACATTTCCACCAAATTTCAATCCTAGTTTAAAATGTTTGCAAGATAAATTTTTA
CAATCAAAACAAAAAAATAAAACTCCACATTTTGCTCTGCAAATTTTTACAACCCTTAAAAATCTTCATAAACTTAACGA
TAACTATAAGCATACTTTACTAAATGCTGCCAAATTATGCCATATTGGAGAATATTTAAATTTCTATTTTGCTAATGAAC
ACTCTGCTCATTTTGTACTAGGTGGATTAAATTATGGATTTTCGCATAAAGAAAAAGCTTTAATTGCTAGTATTATTAAG
CTTAATGGAAAAAAAGTTAATCCTTATAATGTTGAACCCTATAAACAACTTTTACCCAATATTCATACCATTTCATGGCT
TAATTTTATTTTGTGCTTAGCTAAAACTTTAAGCACAAATGAAGATAAGATTGATTTTGCTTTTGCAAACAATACTCTTT
ATATATATCAAGAAAACAAAATTCTTAATTTACCCAAAGATGAGCTCAAAAAAATTGCAAAACCAGCTACCATAGCATTA
GCAATCAATCAAAAAATTTAA

Upstream 100 bases:

>100_bases
CCTGTATTGTTGCTTGCCCATATGAATGTATCATTCCTGATCCTGATAATGTAGAAACTATAGAAGAATTAAAACTCAAA
CATAGAGATAGAGAATTTTA

Downstream 100 bases:

>100_bases
GATTTTTTACCTTTTTGACGCTGCTCTATAAGATTTTGAATTCTTTTTTTATTCTCTTCAAAAAAAGAATCAAAATTTTT
CTCTGTTTTTTCTGTATGTT

Product: Ppx/GppA family phosphatase

Products: NA

Alternate protein names: Guanosine pentaphosphate phosphohydrolase; pppGpp-5'-phosphohydrolase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAKKTAVVDLGSNSIRMVIFEKTSRYGFYTTCEYKRKVRLGENAYNNGKILQEEAMQRAEDALAFFKQCALKHKCKKIFI
VGTSALRDAPNSKNFIKRIKDNLSLNIRCIDGKSESYLGGLAALNLLSPFKDGTTLDIGGGSSELCLIKNNRIISCISLD
IGTVRLKELFYDTGKMDSLEEFIKPILEQIPKEFCNQNLIAIGGSLRAISNSIMQKNSYPLKNLHDFRYMLDEEKGHILK
IFNSNLDSLINFGIKKDRFDTIKEGIFIFLKIAEKIKAKQVITSGVGIREGVYLQDLLRPKITFPPNFNPSLKCLQDKFL
QSKQKNKTPHFALQIFTTLKNLHKLNDNYKHTLLNAAKLCHIGEYLNFYFANEHSAHFVLGGLNYGFSHKEKALIASIIK
LNGKKVNPYNVEPYKQLLPNIHTISWLNFILCLAKTLSTNEDKIDFAFANNTLYIYQENKILNLPKDELKKIAKPATIAL
AINQKI

Sequences:

>Translated_486_residues
MAKKTAVVDLGSNSIRMVIFEKTSRYGFYTTCEYKRKVRLGENAYNNGKILQEEAMQRAEDALAFFKQCALKHKCKKIFI
VGTSALRDAPNSKNFIKRIKDNLSLNIRCIDGKSESYLGGLAALNLLSPFKDGTTLDIGGGSSELCLIKNNRIISCISLD
IGTVRLKELFYDTGKMDSLEEFIKPILEQIPKEFCNQNLIAIGGSLRAISNSIMQKNSYPLKNLHDFRYMLDEEKGHILK
IFNSNLDSLINFGIKKDRFDTIKEGIFIFLKIAEKIKAKQVITSGVGIREGVYLQDLLRPKITFPPNFNPSLKCLQDKFL
QSKQKNKTPHFALQIFTTLKNLHKLNDNYKHTLLNAAKLCHIGEYLNFYFANEHSAHFVLGGLNYGFSHKEKALIASIIK
LNGKKVNPYNVEPYKQLLPNIHTISWLNFILCLAKTLSTNEDKIDFAFANNTLYIYQENKILNLPKDELKKIAKPATIAL
AINQKI
>Mature_485_residues
AKKTAVVDLGSNSIRMVIFEKTSRYGFYTTCEYKRKVRLGENAYNNGKILQEEAMQRAEDALAFFKQCALKHKCKKIFIV
GTSALRDAPNSKNFIKRIKDNLSLNIRCIDGKSESYLGGLAALNLLSPFKDGTTLDIGGGSSELCLIKNNRIISCISLDI
GTVRLKELFYDTGKMDSLEEFIKPILEQIPKEFCNQNLIAIGGSLRAISNSIMQKNSYPLKNLHDFRYMLDEEKGHILKI
FNSNLDSLINFGIKKDRFDTIKEGIFIFLKIAEKIKAKQVITSGVGIREGVYLQDLLRPKITFPPNFNPSLKCLQDKFLQ
SKQKNKTPHFALQIFTTLKNLHKLNDNYKHTLLNAAKLCHIGEYLNFYFANEHSAHFVLGGLNYGFSHKEKALIASIIKL
NGKKVNPYNVEPYKQLLPNIHTISWLNFILCLAKTLSTNEDKIDFAFANNTLYIYQENKILNLPKDELKKIAKPATIALA
INQKI

Specific function: Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in th

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family. GppA subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994966, Length=424, Percent_Identity=24.7641509433962, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788848, Length=437, Percent_Identity=23.1121281464531, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.40 [H]

Molecular weight: Translated: 55180; Mature: 55049

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKTAVVDLGSNSIRMVIFEKTSRYGFYTTCEYKRKVRLGENAYNNGKILQEEAMQRAE
CCCCEEEEEECCCCEEEEEEEECCCCCEEEEECHHHHEECCCCCCCCCCEEHHHHHHHHH
DALAFFKQCALKHKCKKIFIVGTSALRDAPNSKNFIKRIKDNLSLNIRCIDGKSESYLGG
HHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHH
LAALNLLSPFKDGTTLDIGGGSSELCLIKNNRIISCISLDIGTVRLKELFYDTGKMDSLE
HHHHHHHCCCCCCCEEEECCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCHHHH
EFIKPILEQIPKEFCNQNLIAIGGSLRAISNSIMQKNSYPLKNLHDFRYMLDEEKGHILK
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEE
IFNSNLDSLINFGIKKDRFDTIKEGIFIFLKIAEKIKAKQVITSGVGIREGVYLQDLLRP
EECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
KITFPPNFNPSLKCLQDKFLQSKQKNKTPHFALQIFTTLKNLHKLNDNYKHTLLNAAKLC
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HIGEYLNFYFANEHSAHFVLGGLNYGFSHKEKALIASIIKLNGKKVNPYNVEPYKQLLPN
HHHHHHEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCC
IHTISWLNFILCLAKTLSTNEDKIDFAFANNTLYIYQENKILNLPKDELKKIAKPATIAL
CHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEECCCHHHHHHHCCCEEEEE
AINQKI
EEECCC
>Mature Secondary Structure 
AKKTAVVDLGSNSIRMVIFEKTSRYGFYTTCEYKRKVRLGENAYNNGKILQEEAMQRAE
CCCEEEEEECCCCEEEEEEEECCCCCEEEEECHHHHEECCCCCCCCCCEEHHHHHHHHH
DALAFFKQCALKHKCKKIFIVGTSALRDAPNSKNFIKRIKDNLSLNIRCIDGKSESYLGG
HHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHH
LAALNLLSPFKDGTTLDIGGGSSELCLIKNNRIISCISLDIGTVRLKELFYDTGKMDSLE
HHHHHHHCCCCCCCEEEECCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCHHHH
EFIKPILEQIPKEFCNQNLIAIGGSLRAISNSIMQKNSYPLKNLHDFRYMLDEEKGHILK
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEE
IFNSNLDSLINFGIKKDRFDTIKEGIFIFLKIAEKIKAKQVITSGVGIREGVYLQDLLRP
EECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
KITFPPNFNPSLKCLQDKFLQSKQKNKTPHFALQIFTTLKNLHKLNDNYKHTLLNAAKLC
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HIGEYLNFYFANEHSAHFVLGGLNYGFSHKEKALIASIIKLNGKKVNPYNVEPYKQLLPN
HHHHHHEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCC
IHTISWLNFILCLAKTLSTNEDKIDFAFANNTLYIYQENKILNLPKDELKKIAKPATIAL
CHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEECCCHHHHHHHCCCEEEEE
AINQKI
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA