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The map label for this gene is pth [H]

Identifier: 57237363

GI number: 57237363

Start: 318468

End: 319013

Strand: Direct

Name: pth [H]

Synonym: CJE0357

Alternate gene names: 57237363

Gene position: 318468-319013 (Clockwise)

Preceding gene: 57237362

Following gene: 57237364

Centisome position: 17.91

GC content: 31.87

Gene sequence:

>546_bases
ATGATTTTAGTCGTAGGGCTTGGCAATATAGGCGTAGAGTATGAAAATACCCGCCATAATGTCGGTTTTATGCTAATTGA
CTTGCTTTTAAAAGAAAGCAATTTTACAAATCTTACAAACTCTAAATTTAAAGGAGAACTTTTTAAAATAGGTTCTTCTT
TACTTCTTCTTAAACCTAGTACTTATATGAATAATTCAGGACTTAGTGTAAAAGCAGTGAATGATTTTTATAAATGCGAA
AGAATGATAGTTATCCATGATGATATAGATATAAATTTAGGAGCCTTGCGTTTTAAAAAAGGCGGTTCAAGTGGTGGACA
TAATGGGCTTAAAAGCATAGATACTTTATGTGGAAATGATTATGAAAGAGTGCGTATAGGAGTGGGAAAGGGTGAAAATG
TGATTTCCCATGTTTTAGGTAAATTTAAACCCGAAGAAGAAATAACCTTAAGCAAAGTTTTAGAGCATACAAAAAAAGCT
TTGCTTGAGCTTATTGAAAATGATGATTTGTCTGCTATATCATCTAAATACAGCCTAAAGGCTTAA

Upstream 100 bases:

>100_bases
ATGTGGGTGATGCTTTACTTGTTCGTGATATTGTAGTTCCTGCGGGAGTAACTATGATTGATGCTGATAGAGTAGCGGTT
GTAGGTGTAGAAAAAGCTAG

Downstream 100 bases:

>100_bases
GATGTGGATATTTTTTCGTTTTATTTCAGGGATTTATTTAAAAAATTTTTTTATTATATTTTTTTCTTTGCTTGGTTTTT
ATTGCGGTATTGATTTGCTC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MILVVGLGNIGVEYENTRHNVGFMLIDLLLKESNFTNLTNSKFKGELFKIGSSLLLLKPSTYMNNSGLSVKAVNDFYKCE
RMIVIHDDIDINLGALRFKKGGSSGGHNGLKSIDTLCGNDYERVRIGVGKGENVISHVLGKFKPEEEITLSKVLEHTKKA
LLELIENDDLSAISSKYSLKA

Sequences:

>Translated_181_residues
MILVVGLGNIGVEYENTRHNVGFMLIDLLLKESNFTNLTNSKFKGELFKIGSSLLLLKPSTYMNNSGLSVKAVNDFYKCE
RMIVIHDDIDINLGALRFKKGGSSGGHNGLKSIDTLCGNDYERVRIGVGKGENVISHVLGKFKPEEEITLSKVLEHTKKA
LLELIENDDLSAISSKYSLKA
>Mature_181_residues
MILVVGLGNIGVEYENTRHNVGFMLIDLLLKESNFTNLTNSKFKGELFKIGSSLLLLKPSTYMNNSGLSVKAVNDFYKCE
RMIVIHDDIDINLGALRFKKGGSSGGHNGLKSIDTLCGNDYERVRIGVGKGENVISHVLGKFKPEEEITLSKVLEHTKKA
LLELIENDDLSAISSKYSLKA

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=175, Percent_Identity=34.2857142857143, Blast_Score=91, Evalue=5e-19,
Organism=Escherichia coli, GI1787455, Length=190, Percent_Identity=34.7368421052632, Blast_Score=87, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321983, Length=183, Percent_Identity=27.8688524590164, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 19961; Mature: 19961

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVVGLGNIGVEYENTRHNVGFMLIDLLLKESNFTNLTNSKFKGELFKIGSSLLLLKPS
CEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEECCC
TYMNNSGLSVKAVNDFYKCERMIVIHDDIDINLGALRFKKGGSSGGHNGLKSIDTLCGND
CEECCCCCEEEEHHHHHHCCEEEEEECCCCCEECEEEEECCCCCCCCCHHHHHHHHHCCC
YERVRIGVGKGENVISHVLGKFKPEEEITLSKVLEHTKKALLELIENDDLSAISSKYSLK
CEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC
A
C
>Mature Secondary Structure
MILVVGLGNIGVEYENTRHNVGFMLIDLLLKESNFTNLTNSKFKGELFKIGSSLLLLKPS
CEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEECCC
TYMNNSGLSVKAVNDFYKCERMIVIHDDIDINLGALRFKKGGSSGGHNGLKSIDTLCGND
CEECCCCCEEEEHHHHHHCCEEEEEECCCCCEECEEEEECCCCCCCCCHHHHHHHHHCCC
YERVRIGVGKGENVISHVLGKFKPEEEITLSKVLEHTKKALLELIENDDLSAISSKYSLK
CEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA