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The map label for this gene is nlaIIIM [H]

Identifier: 57237212

GI number: 57237212

Start: 202637

End: 203728

Strand: Direct

Name: nlaIIIM [H]

Synonym: CJE0201

Alternate gene names: 57237212

Gene position: 202637-203728 (Clockwise)

Preceding gene: 57237211

Following gene: 57237213

Centisome position: 11.4

GC content: 27.01

Gene sequence:

>1092_bases
TTGAAAGAAAATCCTTCTTTTTTAAAAGAGCAAATTATAACTTATCTTGGAAATAAAAGAGCACTTTTAAGTTTTTTAAA
TAAGGGCTTTAAGGTAGCTAAAAAAGAACTTGGTAAAGATAAATTTAGTTTTTGTGATATATTTAGTGGCTCAGGTATTG
TAAGTCGCTTTGCAAAATCGCATTCAAATTACATTCTAGCAAATGATTTAGAAGATTATTCAAGAATAATAAATGAGTGC
TATTTGAGTAATTTAAATGATGATTTAGAAAAAGATATTAAATATTATTATGAAAAATTGTTAAAAAATTTAGAGCTTAA
AGAAGGTTTTTTAGCTAAGCTTTATGCCCCAAAAGATGATTTAAATATAAAAGCAAATGAAAGGGTTTTTTATACATACA
AAAACGCTTGTTATTTAGATAGTGTTAGAGCAAAAATTGAAAATGAAATTCCACAAGAATTAAAACATTTTTTTATAGCA
CCTTTAATTTATGAAGCTAGTGTGCATTCAAATACTAGTGGAGTTTTTAAAGGTTTTTATAAGGGTAAGGATGGTATCGG
AAAATTTGGAGGAGAAGCGCAAAATGCACTTAAACGCATTAAAGGAGAAATAGAGCTTAAAATCCCTATTTTTTCAAATT
TTTCTTGTGAATTTGAAGTGATGCAAAAGGATGCAAACATGCTTGCAAAAGAACTTGATTTTTTTGATGTAGTGTATCTT
GACCCCCCTTATAATCAGCACCCTTATAGTTCTAATTATTTTATGCTAAATTTAATTGCAAATTATAAAAAACCTGAAGA
AATTTCTAAAATCAGTGGCATTCCAAAGGATTGGAACCGTAGTACTTTTAATAAGTCAAAATTTGCCCAAGATGCTTTAT
TTGAACTTATTAATGATTTAAAAGCAAGAGTGATTTTACTTTCTTATAATTGTGAAGGTTTTGTAAAAAAAGAAATTTTT
TTAAAGCGCTTAAGCACTCTTGGAAAATGCCGGATTTTAGAGCAAAAATATAATACTTTTAGAGCTTCTCGCAATCTTAA
AAATCGTAATATCCATCTTCATGAACAACTTTATATTTTAGTTAAAAATTAA

Upstream 100 bases:

>100_bases
ACAAAGAGCCAAATTTTGAAGGTCGTTATGTAAATATGCTTGTAACTCCTAAAAAGGCTTAATCCACTTTTGTGGATAAG
GCTTTTAAAGGTTTTAAATT

Downstream 100 bases:

>100_bases
ATTTTGTGCTAAAATTATATTTATAAAAAGGAGGGATTTATGAAACTAAAATTATCTTTTTGTACATTAATGGCATTTGG
TTTTTCTAACTATCTTTTTG

Product: D12 class N6 adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.NlaIII; Adenine-specific methyltransferase NlaIII [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGIVSRFAKSHSNYILANDLEDYSRIINEC
YLSNLNDDLEKDIKYYYEKLLKNLELKEGFLAKLYAPKDDLNIKANERVFYTYKNACYLDSVRAKIENEIPQELKHFFIA
PLIYEASVHSNTSGVFKGFYKGKDGIGKFGGEAQNALKRIKGEIELKIPIFSNFSCEFEVMQKDANMLAKELDFFDVVYL
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKSKFAQDALFELINDLKARVILLSYNCEGFVKKEIF
LKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLHEQLYILVKN

Sequences:

>Translated_363_residues
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGIVSRFAKSHSNYILANDLEDYSRIINEC
YLSNLNDDLEKDIKYYYEKLLKNLELKEGFLAKLYAPKDDLNIKANERVFYTYKNACYLDSVRAKIENEIPQELKHFFIA
PLIYEASVHSNTSGVFKGFYKGKDGIGKFGGEAQNALKRIKGEIELKIPIFSNFSCEFEVMQKDANMLAKELDFFDVVYL
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKSKFAQDALFELINDLKARVILLSYNCEGFVKKEIF
LKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLHEQLYILVKN
>Mature_363_residues
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGIVSRFAKSHSNYILANDLEDYSRIINEC
YLSNLNDDLEKDIKYYYEKLLKNLELKEGFLAKLYAPKDDLNIKANERVFYTYKNACYLDSVRAKIENEIPQELKHFFIA
PLIYEASVHSNTSGVFKGFYKGKDGIGKFGGEAQNALKRIKGEIELKIPIFSNFSCEFEVMQKDANMLAKELDFFDVVYL
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKSKFAQDALFELINDLKARVILLSYNCEGFVKKEIF
LKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLHEQLYILVKN

Specific function: This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease [H]

COG id: COG3392

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 42244; Mature: 42244

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGIVSRFAKS
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
HSNYILANDLEDYSRIINECYLSNLNDDLEKDIKYYYEKLLKNLELKEGFLAKLYAPKDD
CCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCC
LNIKANERVFYTYKNACYLDSVRAKIENEIPQELKHFFIAPLIYEASVHSNTSGVFKGFY
CCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KGKDGIGKFGGEAQNALKRIKGEIELKIPIFSNFSCEFEVMQKDANMLAKELDFFDVVYL
CCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEE
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKSKFAQDALFELINDL
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KARVILLSYNCEGFVKKEIFLKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLHEQLYIL
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
VKN
EEC
>Mature Secondary Structure
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGIVSRFAKS
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
HSNYILANDLEDYSRIINECYLSNLNDDLEKDIKYYYEKLLKNLELKEGFLAKLYAPKDD
CCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCC
LNIKANERVFYTYKNACYLDSVRAKIENEIPQELKHFFIAPLIYEASVHSNTSGVFKGFY
CCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KGKDGIGKFGGEAQNALKRIKGEIELKIPIFSNFSCEFEVMQKDANMLAKELDFFDVVYL
CCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEE
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKSKFAQDALFELINDL
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KARVILLSYNCEGFVKKEIFLKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLHEQLYIL
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
VKN
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2277628 [H]