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The map label for this gene is sbmA [H]

Identifier: 57237187

GI number: 57237187

Start: 176030

End: 177229

Strand: Direct

Name: sbmA [H]

Synonym: CJE0175

Alternate gene names: 57237187

Gene position: 176030-177229 (Clockwise)

Preceding gene: 57237186

Following gene: 57237188

Centisome position: 9.9

GC content: 28.83

Gene sequence:

>1200_bases
ATGTTCTCTTCGTTTTTTAAAAGTAAAAAATGGGCTTTATGGGCTTATTTGGGACTTTTTTTACTGTTATTTTTCTTATA
TATACAAACAAGCTTAAATGTTGCGATCAATTCATGGTATAGTGATTTTTACAATGTTTTACAAAAGCCAAAAATAGAAC
TTTTGGATTCTAATTCTACGCAAAAAATAGAAGAAAATCTTGAAAATAATGCCACTTTAATCCAAGAGGCTAATCAAAGA
GCTGAGCAAAATTTCCAAAAAGCTAATTTTATCAATAAAGGTGCATTGTATTATTATCAAAATTTATTAGAATATTTTTT
CAACTCTAGGGCTATGATTGAAAAGCCAAATTATTCAGCAAGTGATTTTTATGCTTTGATTTTAGTTTTTTTGGCTATTG
CTATTCCTTATGTTTTAATCGCTACAATTAATATCTATTTTGCTAGCGTCTATGCTTTTAAGTGGCGTGAGGCTATGACT
TTTTCTTATTTAAAATTTTGGAAAAATAAAGATGATAATATAGAAGGAAGTTCGCAAAGAATTCAAGAAGATACTTATAA
TTTTTCTAAAATCGTAGAGAGTTTAGGACTTAGTTTTATAAAAGCTTTGATGACCCTTGTAGCATTTATTCCGATTTTAT
GGAGTTTAAGTGATGTTGTGAGTAAAGCTTTATTTGCAAATTTAAGCGAAAATTCTTCTTTTTATTTTTTAAAAAACATT
GATGGTTTGCTTGTTTATATTGCACTTTTGATTTCTTTAGGAGGCTTGGTTGTGTCATGGTTTGTAGGTATTAAGCTTCC
TGGACTTGAATATAACAATCAAAAAGCTGAAGCAGCTTTTAGAAAAGAGCTTGTTTATGCTGAGGATAATCGCAAAGAAT
ACGCTAAAAATGAGACTATGATAGAGCTTTTTACAGGGCTTAAATTTAACTATAAAAGACTTTTTTTACATTATGGATAT
TTTAATATTTGGCTCATTTTATTTGAGCAAATGATAGTTATAGTGCCATTTTTAATCATAGCACCAGGGCTTTTTGCTGG
AGCTATAGGACTTGGTATTGTAATGCAGATTAATAATGCATTTGATCAAGTGCGAAGCTCTTTTAGTGTTTTTATCACAA
ACTGGACAACTATCACTCAACTTAGAAGTATATATAAGCGTTTAAAAGAATTTGAGAAAAATATTTCTTATAAGTCTTAG

Upstream 100 bases:

>100_bases
TGTTATGCCTATTGATTACAATATTAAAGACTATTTAGGTAAAAAATAAACTTTTCATTAAAAAAAGTTTATTAGAATTT
TTATTTTAAAGGTGATGATT

Downstream 100 bases:

>100_bases
TTTTTTCTTATAAAATACAAGAAAAAATTCAAATATTAAATTTACTTTTAATTGTGTATAATAAGGTTTAATAATATTTT
ATTTCTTAAGGAGCTTCATT

Product: transporter

Products: ADP; phosphate; microcin B17 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MFSSFFKSKKWALWAYLGLFLLLFFLYIQTSLNVAINSWYSDFYNVLQKPKIELLDSNSTQKIEENLENNATLIQEANQR
AEQNFQKANFINKGALYYYQNLLEYFFNSRAMIEKPNYSASDFYALILVFLAIAIPYVLIATINIYFASVYAFKWREAMT
FSYLKFWKNKDDNIEGSSQRIQEDTYNFSKIVESLGLSFIKALMTLVAFIPILWSLSDVVSKALFANLSENSSFYFLKNI
DGLLVYIALLISLGGLVVSWFVGIKLPGLEYNNQKAEAAFRKELVYAEDNRKEYAKNETMIELFTGLKFNYKRLFLHYGY
FNIWLILFEQMIVIVPFLIIAPGLFAGAIGLGIVMQINNAFDQVRSSFSVFITNWTTITQLRSIYKRLKEFEKNISYKS

Sequences:

>Translated_399_residues
MFSSFFKSKKWALWAYLGLFLLLFFLYIQTSLNVAINSWYSDFYNVLQKPKIELLDSNSTQKIEENLENNATLIQEANQR
AEQNFQKANFINKGALYYYQNLLEYFFNSRAMIEKPNYSASDFYALILVFLAIAIPYVLIATINIYFASVYAFKWREAMT
FSYLKFWKNKDDNIEGSSQRIQEDTYNFSKIVESLGLSFIKALMTLVAFIPILWSLSDVVSKALFANLSENSSFYFLKNI
DGLLVYIALLISLGGLVVSWFVGIKLPGLEYNNQKAEAAFRKELVYAEDNRKEYAKNETMIELFTGLKFNYKRLFLHYGY
FNIWLILFEQMIVIVPFLIIAPGLFAGAIGLGIVMQINNAFDQVRSSFSVFITNWTTITQLRSIYKRLKEFEKNISYKS
>Mature_399_residues
MFSSFFKSKKWALWAYLGLFLLLFFLYIQTSLNVAINSWYSDFYNVLQKPKIELLDSNSTQKIEENLENNATLIQEANQR
AEQNFQKANFINKGALYYYQNLLEYFFNSRAMIEKPNYSASDFYALILVFLAIAIPYVLIATINIYFASVYAFKWREAMT
FSYLKFWKNKDDNIEGSSQRIQEDTYNFSKIVESLGLSFIKALMTLVAFIPILWSLSDVVSKALFANLSENSSFYFLKNI
DGLLVYIALLISLGGLVVSWFVGIKLPGLEYNNQKAEAAFRKELVYAEDNRKEYAKNETMIELFTGLKFNYKRLFLHYGY
FNIWLILFEQMIVIVPFLIIAPGLFAGAIGLGIVMQINNAFDQVRSSFSVFITNWTTITQLRSIYKRLKEFEKNISYKS

Specific function: Required for the uptake of the peptide antibiotic microcin B17 (MccB17) [H]

COG id: COG1133

COG function: function code I; ABC-type long-chain fatty acid transport system, fused permease and ATPase components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti BacA [H]

Homologues:

Organism=Escherichia coli, GI1786575, Length=286, Percent_Identity=42.6573426573427, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1787772, Length=308, Percent_Identity=26.6233766233766, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011527
- InterPro:   IPR009248 [H]

Pfam domain/function: PF05992 SbmA_BacA [H]

EC number: NA

Molecular weight: Translated: 46426; Mature: 46426

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50929 ABC_TM1F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSFFKSKKWALWAYLGLFLLLFFLYIQTSLNVAINSWYSDFYNVLQKPKIELLDSNST
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
QKIEENLENNATLIQEANQRAEQNFQKANFINKGALYYYQNLLEYFFNSRAMIEKPNYSA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCH
SDFYALILVFLAIAIPYVLIATINIYFASVYAFKWREAMTFSYLKFWKNKDDNIEGSSQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IQEDTYNFSKIVESLGLSFIKALMTLVAFIPILWSLSDVVSKALFANLSENSSFYFLKNI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
DGLLVYIALLISLGGLVVSWFVGIKLPGLEYNNQKAEAAFRKELVYAEDNRKEYAKNETM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHH
IELFTGLKFNYKRLFLHYGYFNIWLILFEQMIVIVPFLIIAPGLFAGAIGLGIVMQINNA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDQVRSSFSVFITNWTTITQLRSIYKRLKEFEKNISYKS
HHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFSSFFKSKKWALWAYLGLFLLLFFLYIQTSLNVAINSWYSDFYNVLQKPKIELLDSNST
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
QKIEENLENNATLIQEANQRAEQNFQKANFINKGALYYYQNLLEYFFNSRAMIEKPNYSA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCH
SDFYALILVFLAIAIPYVLIATINIYFASVYAFKWREAMTFSYLKFWKNKDDNIEGSSQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IQEDTYNFSKIVESLGLSFIKALMTLVAFIPILWSLSDVVSKALFANLSENSSFYFLKNI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
DGLLVYIALLISLGGLVVSWFVGIKLPGLEYNNQKAEAAFRKELVYAEDNRKEYAKNETM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHH
IELFTGLKFNYKRLFLHYGYFNIWLILFEQMIVIVPFLIIAPGLFAGAIGLGIVMQINNA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDQVRSSFSVFITNWTTITQLRSIYKRLKEFEKNISYKS
HHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; microcin B17 [Periplasm]; H2O [C]

Specific reaction: ATP + microcin B17 [Periplasm] + H2O = ADP + phosphate + microcin B17 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]