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The map label for this gene is miaA [H]

Identifier: 57237173

GI number: 57237173

Start: 162924

End: 163793

Strand: Direct

Name: miaA [H]

Synonym: CJE0161

Alternate gene names: 57237173

Gene position: 162924-163793 (Clockwise)

Preceding gene: 57237153

Following gene: 57237176

Centisome position: 9.16

GC content: 26.78

Gene sequence:

>870_bases
ATGTTTTTTGAAATAGCATTAATAGGAACAACTGCGAGTGGAAAAACATACATAGCCAATACTTTAGCAAGAGAATTTAA
TGCTGTTGTTTTAAGTCTTGATAGTCTTTGTGTTTATAAAGAGATTAATATAGCAAGTGCCAAGCCTTCACAAGATGATC
TTGCGAGTATAAAATACTTTGGGGTAAATTTATTGAGCGTGAATGAACATTTTAATGTTGAGTTATTCATAAGAGAATAT
CAAAAAGCAAAAGAATTTGCTTTAGCTAGAAATTTACCTCTAATTATAGTAGGTGGAACTGGATTTTATTTAAAAACTAT
GATAGATGGTTTAAGTGAAAAAACCTTAGAGTCAAAGAGTTCTTTAAATAACGATGAAATTTATGCTCTTTTATTAAATA
TTGATCCAAACTATAAAATAGAAAAAAATGATACTTATAGATTAAAAAAATGGCTGGGTATTTATGAACAAACAAGAGAA
ATTCCTAGTGAATTTTTAAAAAGAACACAAAAAACTGGGGTGCTTAAAGATATAGAAATTTATGAGCTTGCGTGGGATAA
AGAAATTTTAAAAAAACGTATTAAAACTAGAACAAAAGAAATGCTTGATAATGGACTTTTAGATGAAGCAAAAATTCTTT
TTTCTAAATTTGATCATAAACTTAAAGCATTAAATTCTATAGGTTTGAAAGAGTGTAAAGAATATTTAGATGGAGAAATT
TCCTTTAAAGAGCTTGAAAATTTAATCACGATACATACTACACAACTTGCCAAAAGACAAAGAACTTTTAATAAAAAATT
TCAAAGTAAAGCCTTGGAATTTGATAAAGCTTTAGCTACTTTAAGAATGAAATTTTCAATCGAAAAATAA

Upstream 100 bases:

>100_bases
ATCTTTAAATTTTATCCAGAATGTGTTCATTTTTCTACTTTAAAATATTTTTTGCTATGATTTTACTAAAATTTTTATAA
ATTTTAGGATAAATATTAAA

Downstream 100 bases:

>100_bases
ATGTTCTATTAGAATTTTTACTCCTAGTATAATTAAAACCAATCCGCCTAAAAGTTCAGCTTTATTTTTAAGATAAATTC
CAAATTTATTCCCTATTTTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MFFEIALIGTTASGKTYIANTLAREFNAVVLSLDSLCVYKEINIASAKPSQDDLASIKYFGVNLLSVNEHFNVELFIREY
QKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKSSLNNDEIYALLLNIDPNYKIEKNDTYRLKKWLGIYEQTRE
IPSEFLKRTQKTGVLKDIEIYELAWDKEILKKRIKTRTKEMLDNGLLDEAKILFSKFDHKLKALNSIGLKECKEYLDGEI
SFKELENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK

Sequences:

>Translated_289_residues
MFFEIALIGTTASGKTYIANTLAREFNAVVLSLDSLCVYKEINIASAKPSQDDLASIKYFGVNLLSVNEHFNVELFIREY
QKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKSSLNNDEIYALLLNIDPNYKIEKNDTYRLKKWLGIYEQTRE
IPSEFLKRTQKTGVLKDIEIYELAWDKEILKKRIKTRTKEMLDNGLLDEAKILFSKFDHKLKALNSIGLKECKEYLDGEI
SFKELENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK
>Mature_289_residues
MFFEIALIGTTASGKTYIANTLAREFNAVVLSLDSLCVYKEINIASAKPSQDDLASIKYFGVNLLSVNEHFNVELFIREY
QKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKSSLNNDEIYALLLNIDPNYKIEKNDTYRLKKWLGIYEQTRE
IPSEFLKRTQKTGVLKDIEIYELAWDKEILKKRIKTRTKEMLDNGLLDEAKILFSKFDHKLKALNSIGLKECKEYLDGEI
SFKELENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=267, Percent_Identity=23.5955056179775, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1790613, Length=283, Percent_Identity=28.6219081272085, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25144712, Length=328, Percent_Identity=23.780487804878, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24649830, Length=265, Percent_Identity=26.0377358490566, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33337; Mature: 33337

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFEIALIGTTASGKTYIANTLAREFNAVVLSLDSLCVYKEINIASAKPSQDDLASIKYF
CEEEEEEEEECCCCCHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
GVNLLSVNEHFNVELFIREYQKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKS
CEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
SLNNDEIYALLLNIDPNYKIEKNDTYRLKKWLGIYEQTREIPSEFLKRTQKTGVLKDIEI
CCCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
YELAWDKEILKKRIKTRTKEMLDNGLLDEAKILFSKFDHKLKALNSIGLKECKEYLDGEI
HHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
SFKELENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MFFEIALIGTTASGKTYIANTLAREFNAVVLSLDSLCVYKEINIASAKPSQDDLASIKYF
CEEEEEEEEECCCCCHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
GVNLLSVNEHFNVELFIREYQKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKS
CEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
SLNNDEIYALLLNIDPNYKIEKNDTYRLKKWLGIYEQTREIPSEFLKRTQKTGVLKDIEI
CCCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
YELAWDKEILKKRIKTRTKEMLDNGLLDEAKILFSKFDHKLKALNSIGLKECKEYLDGEI
HHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
SFKELENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA