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The map label for this gene is dusB [H]

Identifier: 57237130

GI number: 57237130

Start: 124510

End: 125436

Strand: Reverse

Name: dusB [H]

Synonym: CJE0118

Alternate gene names: 57237130

Gene position: 125436-124510 (Counterclockwise)

Preceding gene: 57237131

Following gene: 57237093

Centisome position: 7.06

GC content: 33.76

Gene sequence:

>927_bases
ATGATAGATTTTAGCAAAAAACCCCTGTTTTTAGCCCCTATGGCAGGTTTTTCAGATCTGCCTTTTCGTAATGTAGTAAA
AAAATTTGGTGCTGATATTACCATAAGTGAAATGATAAGTTCTAATGCCTTAGTTTATGAAAGTTCTAAAACTTTACATA
TGCTAGAAAGAGCTGAATTAGAAAATCCCTATATAGTCCAAATTGCAGGTGGAGATAAAGAAGTGCTAAAAAAAGCAGTG
CAAATGCTAAATGAAATGGATTTTGTAGATGGGATCGATTTTAATTGCGGTTGCCCTGTAAATAAAGTTGTTAAACAATG
TGCTGGCAGTGCTTTGCTAGAAAATTTAGAGCTTTTTAAAAATCTTGTAGGAGTGATTAAAGAAAACAATAAAAAAAGCC
TTACAAGTGTGAAATTTCGACTAGGATTTAATGAAAAATATCCTGAAAAAATGGCTAAAATTTGTGAAAGCTTGGGGGTG
GATTTTATAAGTATCCACGGACGCACAAGAAAACAGCTTTATAGCGGAAAAGCCGATTATGGATCCATTGCTAGTGCTAA
AGCGAGTGTGAAAATTCCTGTGATTGCAAATGGCGATATTAACGCACAAAACGCTAAAGAAGTTTATAAAATCACAAAAT
GTGATGGACTCATGATAGGGCGTGCAAGCGTAGGAAATCCTTGGATATTTTATGAAATCAAAAGCGGTAAAAGTGTAGAT
GAAAAACTCAAAAAAGAAATCATACTCACTCATTTTGATGAAATGATAAAACACTACAAAGATCAAGGCGTAAGTATATT
TCGCAAGCATTTACACGAGTATTCTAAAGGGCATAAAGACGCTTCAGCCTTTAGAGATACCGTTAATCGTATCGACAATG
TCGAAGAAATGCGAAAAAAAATAGAAGAATTTTTTAGTTTAACTTGA

Upstream 100 bases:

>100_bases
GAGAACAAATTCACAATGATGATAAAAAATTTAATGATTTTAAAGCCTTTTTAGCCTTGCGTGAAAAAAATATCAAAATC
GATCTAAATCAACTCATACA

Downstream 100 bases:

>100_bases
TTGATTTTTATAATTTAAAAAATCTTAAGCCTTAATATCTATAATTTTTGCTGAAGTGTTTTGAGAAAGAATTTTATTAA
ATTCCCCTGCATTATCATTG

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIDFSKKPLFLAPMAGFSDLPFRNVVKKFGADITISEMISSNALVYESSKTLHMLERAELENPYIVQIAGGDKEVLKKAV
QMLNEMDFVDGIDFNCGCPVNKVVKQCAGSALLENLELFKNLVGVIKENNKKSLTSVKFRLGFNEKYPEKMAKICESLGV
DFISIHGRTRKQLYSGKADYGSIASAKASVKIPVIANGDINAQNAKEVYKITKCDGLMIGRASVGNPWIFYEIKSGKSVD
EKLKKEIILTHFDEMIKHYKDQGVSIFRKHLHEYSKGHKDASAFRDTVNRIDNVEEMRKKIEEFFSLT

Sequences:

>Translated_308_residues
MIDFSKKPLFLAPMAGFSDLPFRNVVKKFGADITISEMISSNALVYESSKTLHMLERAELENPYIVQIAGGDKEVLKKAV
QMLNEMDFVDGIDFNCGCPVNKVVKQCAGSALLENLELFKNLVGVIKENNKKSLTSVKFRLGFNEKYPEKMAKICESLGV
DFISIHGRTRKQLYSGKADYGSIASAKASVKIPVIANGDINAQNAKEVYKITKCDGLMIGRASVGNPWIFYEIKSGKSVD
EKLKKEIILTHFDEMIKHYKDQGVSIFRKHLHEYSKGHKDASAFRDTVNRIDNVEEMRKKIEEFFSLT
>Mature_308_residues
MIDFSKKPLFLAPMAGFSDLPFRNVVKKFGADITISEMISSNALVYESSKTLHMLERAELENPYIVQIAGGDKEVLKKAV
QMLNEMDFVDGIDFNCGCPVNKVVKQCAGSALLENLELFKNLVGVIKENNKKSLTSVKFRLGFNEKYPEKMAKICESLGV
DFISIHGRTRKQLYSGKADYGSIASAKASVKIPVIANGDINAQNAKEVYKITKCDGLMIGRASVGNPWIFYEIKSGKSVD
EKLKKEIILTHFDEMIKHYKDQGVSIFRKHLHEYSKGHKDASAFRDTVNRIDNVEEMRKKIEEFFSLT

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=273, Percent_Identity=31.1355311355311, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI239788462, Length=273, Percent_Identity=31.1355311355311, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=31.5315315315315, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=28.2608695652174, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8923374, Length=262, Percent_Identity=28.2442748091603, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789660, Length=310, Percent_Identity=38.0645161290323, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=29.4871794871795, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=291, Percent_Identity=30.5841924398625, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=221, Percent_Identity=31.2217194570136, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=225, Percent_Identity=27.5555555555556, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=249, Percent_Identity=27.710843373494, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=31.4655172413793, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323433, Length=309, Percent_Identity=27.1844660194175, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=314, Percent_Identity=25.1592356687898, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24585320, Length=283, Percent_Identity=30.7420494699647, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=28.8888888888889, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24580595, Length=223, Percent_Identity=28.6995515695067, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19920448, Length=223, Percent_Identity=28.6995515695067, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34666; Mature: 34666

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFSKKPLFLAPMAGFSDLPFRNVVKKFGADITISEMISSNALVYESSKTLHMLERAEL
CCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCEEEECCCHHHHHHHHCC
ENPYIVQIAGGDKEVLKKAVQMLNEMDFVDGIDFNCGCPVNKVVKQCAGSALLENLELFK
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLVGVIKENNKKSLTSVKFRLGFNEKYPEKMAKICESLGVDFISIHGRTRKQLYSGKADY
HHHHHHHCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCC
GSIASAKASVKIPVIANGDINAQNAKEVYKITKCDGLMIGRASVGNPWIFYEIKSGKSVD
CCHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCHH
EKLKKEIILTHFDEMIKHYKDQGVSIFRKHLHEYSKGHKDASAFRDTVNRIDNVEEMRKK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
IEEFFSLT
HHHHHCCC
>Mature Secondary Structure
MIDFSKKPLFLAPMAGFSDLPFRNVVKKFGADITISEMISSNALVYESSKTLHMLERAEL
CCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCEEEECCCHHHHHHHHCC
ENPYIVQIAGGDKEVLKKAVQMLNEMDFVDGIDFNCGCPVNKVVKQCAGSALLENLELFK
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLVGVIKENNKKSLTSVKFRLGFNEKYPEKMAKICESLGVDFISIHGRTRKQLYSGKADY
HHHHHHHCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCC
GSIASAKASVKIPVIANGDINAQNAKEVYKITKCDGLMIGRASVGNPWIFYEIKSGKSVD
CCHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCHH
EKLKKEIILTHFDEMIKHYKDQGVSIFRKHLHEYSKGHKDASAFRDTVNRIDNVEEMRKK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
IEEFFSLT
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]