Click here to switch to the map view.

The map label for this gene is aspA [H]

Identifier: 57237094

GI number: 57237094

Start: 95082

End: 96488

Strand: Direct

Name: aspA [H]

Synonym: CJE0082

Alternate gene names: 57237094

Gene position: 95082-96488 (Clockwise)

Preceding gene: 57237091

Following gene: 57237095

Centisome position: 5.35

GC content: 33.4

Gene sequence:

>1407_bases
ATGGGAACAAGAAAAGAACATGATTTTATTGGGGAATTAGAAATCTCTGATGAGGTTTATTATGGAGTGCAAACTTTTAG
AGCTGTTGAAAATTTTGATATTTCTCATGATAGATTAAAAGATTTTCCTCGCTTTGTTAGAGCTTTGGCTAGAGTAAAAA
AAGCTGCAGCTATGGCAAATCATGAATTAGGTCTTTTGGATAAAAATATCCAAGATGCGATTATTAAAGCGTGCGATAAA
ATTTTAGAAGGCGGGTATTATGATCAATTTGTAGTAGATATGATCCAAGGAGGTGCTGGAACAAGTACAAATATGAATGC
TAATGAAGTTATAGCGAACATAGGACTTGAACTTATGGGTCATAAAAAAGGTGAATACCAATATCTTCATCCAAATGATC
ATGTTAATTTAAGCCAAAGTACTAATGATGCTTATCCTACGGCTTTACACCTTGCCTTGCATGATTATTTAAGTGATTTG
GCAAAAGCTATGGAACATCTAAAAAAAGCTTATGAGAGAAAAGCTGAAGAATTTAAAGATGTTCTTAAAATGGGTAGAAC
CCAACTGCAAGATGCTGTACCTATGACTTTAGGTCGTGAATTTAAAACTTTTGCCGTAATGATAGGTGAAGATATACAAA
GAGTATTAGAAGCTAGAAAATTGATTTTAGAGATCAATTTGGGTGGAACTGCTATTGGAACGGGAATTAATTCTCATCCT
GATTATCCGAAGGTTGTAGAAAGAAAAATAAGAGAAGTGACAGGTTTTGAATATACTGTGGCTGAGGATTTGATCGAGGC
GACTCAAGATACGGGAGCTTATGTACAAATTTCAGGTGTTTTAAAACGTGTTGCAACAAAACTTTCTAAAGTATGTAATG
ACTTAAGACTTTTAAGTAGTGGTCCAAAATGTGGTCTTAATGAGATTAATCTTCCAAAAATGCAACCAGGTAGTTCTATC
ATGCCAGGTAAGGTAAATCCTGTTATTCCTGAAGTAGTTAATCAAGTTTGTTATTTTGTTATTGGAGCAGACGTAACTGT
AACTTTTGCTTGTGAGGGTGGACAATTACAACTTAATGTTTTTGAACCAGTTGTAGCATATAGCTTATTTAATTCTGTTG
TAATGCTAGAAAAAGCAATGTATACTTTAGCTGATAAATGTATAGATGGTATTACCGCAAATGAAAAAATTTGTTCAGAC
TTTGTCTATAATTCAGTAGGTATAGTAACAGCTTTAAATCCTTATATTGGTTATGAAAATTCAGCTTCTATAGCTAAAGA
GGCAATGAACACTGGAAAAAGAGTGGCTGATATTGCGCTTGAAAGAGGGCTTTTAAGTAAAGAACAAATTGATGAAATTT
TAACTCCATCAAATATGCTAAATCCTCACATGGAAGCTAAAAAATAG

Upstream 100 bases:

>100_bases
TTATTAACCAATTATTAACTAAAAATGCAATATCATTATTTTATGATTTAAATCAAAAATTTTTGATTTTAAATTAAAAT
ACTTTTATGAAAGGTTCAAA

Downstream 100 bases:

>100_bases
CATTTTAGGATTTAATATGGATATAATGATAATTTTGCAAGTAATCGTCCTTCTTGGGGCGATTTTTATCGGTATTCGCC
TAGGCGGCATTGCTATAGGT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMANHELGLLDKNIQDAIIKACDK
ILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMGHKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDL
AKAMEHLKKAYERKAEEFKDVLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSSGPKCGLNEINLPKMQPGSSI
MPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNVFEPVVAYSLFNSVVMLEKAMYTLADKCIDGITANEKICSD
FVYNSVGIVTALNPYIGYENSASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMEAKK

Sequences:

>Translated_468_residues
MGTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMANHELGLLDKNIQDAIIKACDK
ILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMGHKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDL
AKAMEHLKKAYERKAEEFKDVLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSSGPKCGLNEINLPKMQPGSSI
MPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNVFEPVVAYSLFNSVVMLEKAMYTLADKCIDGITANEKICSD
FVYNSVGIVTALNPYIGYENSASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMEAKK
>Mature_467_residues
GTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMANHELGLLDKNIQDAIIKACDKI
LEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMGHKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDLA
KAMEHLKKAYERKAEEFKDVLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHPD
YPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSSGPKCGLNEINLPKMQPGSSIM
PGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNVFEPVVAYSLFNSVVMLEKAMYTLADKCIDGITANEKICSDF
VYNSVGIVTALNPYIGYENSASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMEAKK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=40.4347826086956, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=58.1545064377682, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1787896, Length=458, Percent_Identity=39.0829694323144, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1790398, Length=331, Percent_Identity=22.9607250755287, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=41.7391304347826, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=40.9785932721713, Blast_Score=275, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=41.5217391304348, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=40.997830802603, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=40.997830802603, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=40.1727861771058, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=40.8207343412527, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24583245, Length=466, Percent_Identity=35.6223175965665, Blast_Score=307, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51766; Mature: 51635

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMAN
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HELGLLDKNIQDAIIKACDKILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHC
HKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDLAKAMEHLKKAYERKAEEFKD
CCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
HHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECCCCCCCC
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPKCGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCEEEEEEECCCEEEEEH
FEPVVAYSLFNSVVMLEKAMYTLADKCIDGITANEKICSDFVYNSVGIVTALNPYIGYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHEECCCCCCCCC
SASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMEAKK
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure 
GTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMAN
CCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HELGLLDKNIQDAIIKACDKILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHC
HKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDLAKAMEHLKKAYERKAEEFKD
CCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
HHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECCCCCCCC
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPKCGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCEEEEEEECCCEEEEEH
FEPVVAYSLFNSVVMLEKAMYTLADKCIDGITANEKICSDFVYNSVGIVTALNPYIGYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHEECCCCCCCCC
SASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMEAKK
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]