Click here to switch to the map view.

The map label for this gene is rpoN [H]

Identifier: 57236979

GI number: 57236979

Start: 702970

End: 704220

Strand: Direct

Name: rpoN [H]

Synonym: CJE0771

Alternate gene names: 57236979

Gene position: 702970-704220 (Clockwise)

Preceding gene: 57236978

Following gene: 57236980

Centisome position: 39.54

GC content: 29.26

Gene sequence:

>1251_bases
ATGTTAAAGCAAAAAATCACCCAAGCGCCCAAGACTAAAATTTCTCAAACTTTACGCTCTTGGTTGCCTATTTTACAAGC
AAATATAGAGGATTTAAAAGAAAATTTAGATAAATTTGCTGAGGATAATCCTTTCTTAAATGTTCAAGATTCGATACAAA
CTCATGATAAGGGTAAGAATTATTTTGATTCATTTTATAAACATAATGTAAATTCGGCTTTTGTGGATAGTAAAGGACTT
GCCAAAAAAAGTGTTTATGAGCTTTTAAATGAACAGATTCTTCCTCCTTTGTTTCCTACAAACAAATCTCAAGAACTTGC
AAAAAAAATTATAGAATGCTTAAATGAGGAAGGATATTTTGAATACGATGAAGAATTTTTAAAAGAGTATTCTTTGGAAG
AAATTGAGCGAGTAAGAGCAAGATTTAAATTTTTAGATCCTGTAGGAGTGGGAGCTAAAGATTATAAAGAAGCATTTTTA
TTTGCTCTTGAAAATATGGAATTAGATGAAGATATTGATGAGTTTTGTAGAATGTTGATTATGGATTTTGAAAATATACA
AAACTATACCAAAGAGCCTTTATATAAAGAAGCATTAGCTGTACTTAAGCGCTTTAGCACTCCTCCTTTTTTGGAATATT
TTGAAGATTCTCGTATAATTGTTCCTGATATTTTTGTTTATAAAGAAAATGGTGAAATTAAAGTAAAAATCAATGATGAT
TATTATCCTGAAATTTCAATCCAAACTGATGGTTTAGAGCATGATTTTTTAAGTCATTACATCAAAGAAGCAAAAAATTT
AGTCGATGCTCTAGCGATGAGAAAAGCTACTCTTTATAAAATAGGGCTTATGATAGTAGAATATCAATATGATTTTTTTA
TGGGAAAAGAGATAAAACCTATGACTTTTAAGGATTTAGCACTTGATTTAGAACGCAATGCTTCGACTATATCAAGAGCT
GTGGCAAATAAATATTTAAGTTGTGAAAGAGGACTTATCCCTTTGCGTGATTTTTTTGCTTTTGCTCTTGATGAAGAGGG
AGAGACTTCAAATGTAGGTGTTAAAGAATTTGTAGCAAATTTGGTAAAGAATGAAGATCGAAATAAACCTTTAAGTGATA
GTAAAATTTTAGAATTAATCAAAGAAGAATTTAAAGTCGATATAGGGCGAAGAACTATTACAAAATATCGCAAACATCTT
AATATAGCAAGCTCTACAGATAGAAAAAAATTATATGAACTTGAAGGATAA

Upstream 100 bases:

>100_bases
GATCAGGTTCTTTACTTGCTAGTGGGAATGCTGATGAAATTGCAACCAATAAAGACGTGAAAAAGTATTATTTAGGAGCG
GAGTTTAAACTCCTTGATTG

Downstream 100 bases:

>100_bases
AATTCTATCAATCTATCAAACCCATTACACTTTTGTTTTATATTTAAATTTAATTTAAATAATATAAAAAATTATTGTTT
TATTTTTAGAAATATTATTC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLKQKITQAPKTKISQTLRSWLPILQANIEDLKENLDKFAEDNPFLNVQDSIQTHDKGKNYFDSFYKHNVNSAFVDSKGL
AKKSVYELLNEQILPPLFPTNKSQELAKKIIECLNEEGYFEYDEEFLKEYSLEEIERVRARFKFLDPVGVGAKDYKEAFL
FALENMELDEDIDEFCRMLIMDFENIQNYTKEPLYKEALAVLKRFSTPPFLEYFEDSRIIVPDIFVYKENGEIKVKINDD
YYPEISIQTDGLEHDFLSHYIKEAKNLVDALAMRKATLYKIGLMIVEYQYDFFMGKEIKPMTFKDLALDLERNASTISRA
VANKYLSCERGLIPLRDFFAFALDEEGETSNVGVKEFVANLVKNEDRNKPLSDSKILELIKEEFKVDIGRRTITKYRKHL
NIASSTDRKKLYELEG

Sequences:

>Translated_416_residues
MLKQKITQAPKTKISQTLRSWLPILQANIEDLKENLDKFAEDNPFLNVQDSIQTHDKGKNYFDSFYKHNVNSAFVDSKGL
AKKSVYELLNEQILPPLFPTNKSQELAKKIIECLNEEGYFEYDEEFLKEYSLEEIERVRARFKFLDPVGVGAKDYKEAFL
FALENMELDEDIDEFCRMLIMDFENIQNYTKEPLYKEALAVLKRFSTPPFLEYFEDSRIIVPDIFVYKENGEIKVKINDD
YYPEISIQTDGLEHDFLSHYIKEAKNLVDALAMRKATLYKIGLMIVEYQYDFFMGKEIKPMTFKDLALDLERNASTISRA
VANKYLSCERGLIPLRDFFAFALDEEGETSNVGVKEFVANLVKNEDRNKPLSDSKILELIKEEFKVDIGRRTITKYRKHL
NIASSTDRKKLYELEG
>Mature_416_residues
MLKQKITQAPKTKISQTLRSWLPILQANIEDLKENLDKFAEDNPFLNVQDSIQTHDKGKNYFDSFYKHNVNSAFVDSKGL
AKKSVYELLNEQILPPLFPTNKSQELAKKIIECLNEEGYFEYDEEFLKEYSLEEIERVRARFKFLDPVGVGAKDYKEAFL
FALENMELDEDIDEFCRMLIMDFENIQNYTKEPLYKEALAVLKRFSTPPFLEYFEDSRIIVPDIFVYKENGEIKVKINDD
YYPEISIQTDGLEHDFLSHYIKEAKNLVDALAMRKATLYKIGLMIVEYQYDFFMGKEIKPMTFKDLALDLERNASTISRA
VANKYLSCERGLIPLRDFFAFALDEEGETSNVGVKEFVANLVKNEDRNKPLSDSKILELIKEEFKVDIGRRTITKYRKHL
NIASSTDRKKLYELEG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=478, Percent_Identity=28.8702928870293, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 48627; Mature: 48627

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQKITQAPKTKISQTLRSWLPILQANIEDLKENLDKFAEDNPFLNVQDSIQTHDKGKN
CCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHCCHH
YFDSFYKHNVNSAFVDSKGLAKKSVYELLNEQILPPLFPTNKSQELAKKIIECLNEEGYF
HHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
EYDEEFLKEYSLEEIERVRARFKFLDPVGVGAKDYKEAFLFALENMELDEDIDEFCRMLI
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
MDFENIQNYTKEPLYKEALAVLKRFSTPPFLEYFEDSRIIVPDIFVYKENGEIKVKINDD
HHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHCCCCEEECEEEEEECCCEEEEEECCC
YYPEISIQTDGLEHDFLSHYIKEAKNLVDALAMRKATLYKIGLMIVEYQYDFFMGKEIKP
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCC
MTFKDLALDLERNASTISRAVANKYLSCERGLIPLRDFFAFALDEEGETSNVGVKEFVAN
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
LVKNEDRNKPLSDSKILELIKEEFKVDIGRRTITKYRKHLNIASSTDRKKLYELEG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MLKQKITQAPKTKISQTLRSWLPILQANIEDLKENLDKFAEDNPFLNVQDSIQTHDKGKN
CCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHCCHH
YFDSFYKHNVNSAFVDSKGLAKKSVYELLNEQILPPLFPTNKSQELAKKIIECLNEEGYF
HHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
EYDEEFLKEYSLEEIERVRARFKFLDPVGVGAKDYKEAFLFALENMELDEDIDEFCRMLI
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
MDFENIQNYTKEPLYKEALAVLKRFSTPPFLEYFEDSRIIVPDIFVYKENGEIKVKINDD
HHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHCCCCEEECEEEEEECCCEEEEEECCC
YYPEISIQTDGLEHDFLSHYIKEAKNLVDALAMRKATLYKIGLMIVEYQYDFFMGKEIKP
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCC
MTFKDLALDLERNASTISRAVANKYLSCERGLIPLRDFFAFALDEEGETSNVGVKEFVAN
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
LVKNEDRNKPLSDSKILELIKEEFKVDIGRRTITKYRKHLNIASSTDRKKLYELEG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]