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The map label for this gene is metX [H]

Identifier: 57236912

GI number: 57236912

Start: 1772817

End: 1774088

Strand: Reverse

Name: metX [H]

Synonym: CJE1895

Alternate gene names: 57236912

Gene position: 1774088-1772817 (Counterclockwise)

Preceding gene: 57236913

Following gene: 57236911

Centisome position: 99.79

GC content: 33.96

Gene sequence:

>1272_bases
ATGAATTTCAATAAAGAAACTTTAGCATTACACGGAGCTTATAATTTTGATACTCAAAGAAGTATTAGTGTGCCTATATA
TCAAAACACTGCGTATAATTTTGAAAATTTGGATCAAGCTGCAGCAAGGTTTAATCTTCAAGAACTTGGCAATATTTACT
CAAGACTTAGCAATCCTACAAGCGATGTTTTAGGACAAAGACTTGCTAATGTCGAAGGAGGGGCTTTTGGAATTCCTGTT
GCTAGCGGTATGGCAGCTTGTTTTTATGCTCTTATCAATTTAGCAAGTTCGGGAGATAATGTCGCGTATTCGAACAAAAT
TTATGGTGGGACTCAAACTTTAATTTCTCACACACTTAAAAATTTTGGCATAGAAGCTAGGGAATTTGATATCGATGATT
TAGATAGCTTGGAAAAAGTTATAGATCAAAACACAAAAGCGATTTTTTTCGAAAGTCTTTCAAATCCTCAAATTGCCATA
GCTGATATAGAAAAAATAAGCCAAATAGCAAAAAAACATAAAATCGTTAGCATTTGTGATAATACCGTTGCTACTCCTTT
CTTACTCCAACCTTTTAAACATGGCGTGGATGTAATTGTGCATAGTTTAAGTAAATATGTAAGCGGTCAAGGCAGTGCTT
TGGGTGGAGTACTTATAGAAAGAAAAGATTTAAACGACTTGCTTAAAAATAACGATAGATATAAAGCTTTTAACACTCCT
GATCCAAGTTATCATGGACTGAATTTAAATACACTTGATTTGCCGATTTTTAGTATTAGAGTCATCATCACTTGGCTTAG
AGATCTAGGAGCTAGCTTAGCACCTCAAAATGCTTGGTTACTTTTACAAGGACTTGAAACCTTGGCAGTGCGTATAGAAA
AACACAGTCAAAATGCTGAAAAAGTTGCGAATTTTTTAAATTCTCATCCTGATATCAAGGGCGTAAATTATCCTACTTTA
GCAAGTAATGCTTATCATAATTTATTTAAAAAATATTTTGATAAAAATTTTGCTAGCGGGCTTTTAAGCTTTGAAGCTAA
AGATTATGAGCATGCTAGAAGAATTTGTGATAAAACTCAACTTTTCTTACTTGCTGCAAATTTGGGTGATAGCAAGTCTT
TGATCATCCATCCTGCTTCTACTACTCATTCGCAACTAAGCGAAGAAGAACTCCAAAAAGCAGGCATTACGAAAGCTACT
ATACGCTTAAGCATAGGACTTGAAAATAGCGATGATTTGATAGCGGATTTAAAACAAGCTATAGAAAGTTAA

Upstream 100 bases:

>100_bases
TATATACTCATATTCTATAAATTTACATTTTCAAATAGCCTAGTTTAAAGCCGATAAAAAGCTTTTTAACATTTTTTCAT
TGACACAATAAGGATAAAAA

Downstream 100 bases:

>100_bases
AAGTGTGTTTGCATTGAGTGGAATGAGATAAAAATTCCATCATGCTTTATCATAAATAAGGTTTATTAAATAAAGGAGAA
AAAATGCCACTGATTATTCC

Product: homoserine O-acetyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNFNKETLALHGAYNFDTQRSISVPIYQNTAYNFENLDQAAARFNLQELGNIYSRLSNPTSDVLGQRLANVEGGAFGIPV
ASGMAACFYALINLASSGDNVAYSNKIYGGTQTLISHTLKNFGIEAREFDIDDLDSLEKVIDQNTKAIFFESLSNPQIAI
ADIEKISQIAKKHKIVSICDNTVATPFLLQPFKHGVDVIVHSLSKYVSGQGSALGGVLIERKDLNDLLKNNDRYKAFNTP
DPSYHGLNLNTLDLPIFSIRVIITWLRDLGASLAPQNAWLLLQGLETLAVRIEKHSQNAEKVANFLNSHPDIKGVNYPTL
ASNAYHNLFKKYFDKNFASGLLSFEAKDYEHARRICDKTQLFLLAANLGDSKSLIIHPASTTHSQLSEEELQKAGITKAT
IRLSIGLENSDDLIADLKQAIES

Sequences:

>Translated_423_residues
MNFNKETLALHGAYNFDTQRSISVPIYQNTAYNFENLDQAAARFNLQELGNIYSRLSNPTSDVLGQRLANVEGGAFGIPV
ASGMAACFYALINLASSGDNVAYSNKIYGGTQTLISHTLKNFGIEAREFDIDDLDSLEKVIDQNTKAIFFESLSNPQIAI
ADIEKISQIAKKHKIVSICDNTVATPFLLQPFKHGVDVIVHSLSKYVSGQGSALGGVLIERKDLNDLLKNNDRYKAFNTP
DPSYHGLNLNTLDLPIFSIRVIITWLRDLGASLAPQNAWLLLQGLETLAVRIEKHSQNAEKVANFLNSHPDIKGVNYPTL
ASNAYHNLFKKYFDKNFASGLLSFEAKDYEHARRICDKTQLFLLAANLGDSKSLIIHPASTTHSQLSEEELQKAGITKAT
IRLSIGLENSDDLIADLKQAIES
>Mature_423_residues
MNFNKETLALHGAYNFDTQRSISVPIYQNTAYNFENLDQAAARFNLQELGNIYSRLSNPTSDVLGQRLANVEGGAFGIPV
ASGMAACFYALINLASSGDNVAYSNKIYGGTQTLISHTLKNFGIEAREFDIDDLDSLEKVIDQNTKAIFFESLSNPQIAI
ADIEKISQIAKKHKIVSICDNTVATPFLLQPFKHGVDVIVHSLSKYVSGQGSALGGVLIERKDLNDLLKNNDRYKAFNTP
DPSYHGLNLNTLDLPIFSIRVIITWLRDLGASLAPQNAWLLLQGLETLAVRIEKHSQNAEKVANFLNSHPDIKGVNYPTL
ASNAYHNLFKKYFDKNFASGLLSFEAKDYEHARRICDKTQLFLLAANLGDSKSLIIHPASTTHSQLSEEELQKAGITKAT
IRLSIGLENSDDLIADLKQAIES

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=410, Percent_Identity=30.2439024390244, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI299473759, Length=410, Percent_Identity=27.8048780487805, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI25453487, Length=409, Percent_Identity=26.8948655256724, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1790375, Length=425, Percent_Identity=29.8823529411765, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1789383, Length=377, Percent_Identity=27.5862068965517, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71980708, Length=407, Percent_Identity=34.3980343980344, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=377, Percent_Identity=34.7480106100796, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=402, Percent_Identity=34.0796019900498, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=420, Percent_Identity=39.5238095238095, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=28.8416075650118, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=432, Percent_Identity=26.1574074074074, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=338, Percent_Identity=25.7396449704142, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=425, Percent_Identity=24.4705882352941, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19922554, Length=379, Percent_Identity=31.6622691292876, Blast_Score=184, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46694; Mature: 46694

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNKETLALHGAYNFDTQRSISVPIYQNTAYNFENLDQAAARFNLQELGNIYSRLSNPT
CCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCH
SDVLGQRLANVEGGAFGIPVASGMAACFYALINLASSGDNVAYSNKIYGGTQTLISHTLK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHH
NFGIEAREFDIDDLDSLEKVIDQNTKAIFFESLSNPQIAIADIEKISQIAKKHKIVSICD
HCCCCCEECCCCCHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCHHHHHC
NTVATPFLLQPFKHGVDVIVHSLSKYVSGQGSALGGVLIERKDLNDLLKNNDRYKAFNTP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCCCEEECCCC
DPSYHGLNLNTLDLPIFSIRVIITWLRDLGASLAPQNAWLLLQGLETLAVRIEKHSQNAE
CCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
KVANFLNSHPDIKGVNYPTLASNAYHNLFKKYFDKNFASGLLSFEAKDYEHARRICDKTQ
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHE
LFLLAANLGDSKSLIIHPASTTHSQLSEEELQKAGITKATIRLSIGLENSDDLIADLKQA
EEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHH
IES
HCC
>Mature Secondary Structure
MNFNKETLALHGAYNFDTQRSISVPIYQNTAYNFENLDQAAARFNLQELGNIYSRLSNPT
CCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCH
SDVLGQRLANVEGGAFGIPVASGMAACFYALINLASSGDNVAYSNKIYGGTQTLISHTLK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHH
NFGIEAREFDIDDLDSLEKVIDQNTKAIFFESLSNPQIAIADIEKISQIAKKHKIVSICD
HCCCCCEECCCCCHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCHHHHHC
NTVATPFLLQPFKHGVDVIVHSLSKYVSGQGSALGGVLIERKDLNDLLKNNDRYKAFNTP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCCCEEECCCC
DPSYHGLNLNTLDLPIFSIRVIITWLRDLGASLAPQNAWLLLQGLETLAVRIEKHSQNAE
CCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
KVANFLNSHPDIKGVNYPTLASNAYHNLFKKYFDKNFASGLLSFEAKDYEHARRICDKTQ
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHE
LFLLAANLGDSKSLIIHPASTTHSQLSEEELQKAGITKATIRLSIGLENSDDLIADLKQA
EEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHH
IES
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]